اثر آزمایشگاهی عصاره آبی و اتانولی بابونه شیرازی (Matricaria chamomile) بر فعالیت حیاتی لکوسیت‌های خون محیطی بیماران مبتلا به کووید- 19

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه ایمونولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

2 مرکز تحقیقات تنظیم پاسخ‌های ایمنی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: مطالعات متعدد اثرات ضدالتهاب، ضدمیکروب، ضدحساسیت، ضدسرطان، ضداسپاسم، مسکن و ترمیم کنندگی زخم بابونه (Matricaria chamomile) را نشان داده‌اند. در این مطالعه تاثیر غلظت‌های مناسب عصاره آبی و اتانولی بابونه شیرازی بر روی فعالیت حیاتی لکوسیت‌های بیماران مبتلا به کووید-19 مورد بررسی قرار گرفت.
مواد و روش ها: پس از تهیه عصاره آبی و اتانولی بابونه، سلول‌های تک هسته‌ای خون محیطی(PBMC) 3 بیمار مبتلا به کووید-19 با دوزهای 200، 300، 400 و 500 میکروگرم بر میلی‌لیتر عصاره اتانولی بابونه و  دوزهای 400، 500، 600 و 700 میکروگرم بر میلی‌لیتر عصاره آبی بابونه تیمار شدند. پس از گذشت 24 ساعت، فعالیت حیاتی PBMCs به روش‌ MTT سنجیده شد. تجزیه و تحلیل آماری داده‌ها به وسیله نرم افزار SPSS24  بوسیله آزمون‌ آماری ANOVA one-way  انجام شد. مقادیر P<0.05 معنی‌دار در نظر گرفته شد.
نتایج: در تحقیق حاضر، عصاره اتانولی بابونه در دوز 500 میکروگرم بر میلی‌لیتر و عصاره آبی در دوزهای 600 و 700 میکروگرم بر میلی‌لیتر منجر به کاهش معنی‌دار فعالیت حیاتی سلول‌های تک هسته‌ای خون محیطی نسبت به گروه کنترل شدند.
نتیجه‌گیری: کاهش فعالیت حیاتی سلول‌های تک هسته‌ای خون محیطی در غلظت‌های مختلف عصاره‌های آبی و اتانولی بابونه وابسته به دوز بوده و با افزایش دوز کاهش فعالیت حیاتی مشاهده می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of aqueous and ethanolic extracts of chamomile on the vital activity of peripheral blood leukocytes of patients with covid-19

نویسندگان [English]

  • Rasoul Rashidi 1
  • Tooba Ghazanfari 2
  • Tayabeh Radjabian 2
  • Ensie Sadat Mirsharif 2
1 Department of immunology, Faculty of Medicine, Shahed University, Tehran, Iran
2 Immunoregulation Research Center, Shahed University, Tehran, Iran
چکیده [English]

Background and Objective: Numerous studies have shown the anti-inflammatory, antimicrobial, anti-allergic, anti-cancer, anti-spasm, analgesic and wound healing effects of chamomile (Matricaria chamomile). In this study, the effect of aqueous and ethanolic extracts of chamomile on the vital activity of cultured leukocytes of patients with covid-19 was investigated.
Materials and Methods: After preparation of aqueous and ethanolic extracts of chamomile, peripheral blood mononuclear cells (PBMC) of patients with covid-19 with doses of 200, 300, 400 and 500 micrograms/ml of ethanolic extract of chamomile and doses of 400, 500, 600 and 700 µg/ml chamomile aqueous extract were treated. After 24 hours, the vital activity of PBMCs was measured by MTT method. To analyze the findings, one-way analysis of variance was used in SPSS software version 24. P<0.05 was considered significant.
Results: In the current research, chamomile ethanol extract in doses of 500 micrograms/ml and aqueous extract in doses of 600 and 700 micrograms/ml led to a significant decrease in the vital activity of peripheral blood mononuclear cells compared to the control group.
Conclusion: Aqueous and ethanol extracts of chamomile significantly decrease the vital activity of peripheral blood mononuclear cells; This decrease in vital activity is dose-dependent, and with increasing dose, a decrease in vital activity is observed.

کلیدواژه‌ها [English]

  • Chamomile
  • Covid-19
  • Peripheral blood mononuclear cells
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