اثر تمرین شنا و سلول های بنیادی بر بیان ژن های DAZL و VASA در موش های سفید بزرگ آزمایشگاهی مدل آزواسپرمی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تربیت بدنی و‌علوم ورزشی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه فیزیولوژی ورزشی،واحد ساری،دانشگاه آزاد اسلامی، ساری، ایران

چکیده

مقدمه و هدف: اسپرماتوژنز فرآیندی است که با تکثیر و تمایز سلولهای بنیادی اسپرماتوگونی صورت می پذیرد. این سلولها روی غشای پایه لوله های منی ساز واقع شده اند و سلولهای سرتولی آنها را احاطه کرده اند. این مجموعه محیطی را فراهم می آورد که باعث عملکرد و بقای اسپرماتوژنز میشود. هدف از تحقیق حاضر بررسی اثر تمرین شنا و سلول های بنیادی بر بیان ژن های DAZL و VASA در موش های سفید بزرگ آزمایشگاهی مدل آزواسپرمی بود.
مواد و روش ها: روش تحقیق از نظر کنترل متغیرهای تحقیق تجربی و از نظر هدف کاربردی است.  25 سر رت 8 هفته ای و پس از القاء مدل آزواسپرمی که با بررسی‌های بافت‌شناسی انحطاط مشخص سلول‌های اسپرم‌ساز را نشان داد که منجر به ایجاد اپیتلیوم منی‌ساز با دیواره نازک در اکثر لوله‌های منی‌ساز می‌شود که نشان‌دهنده اختلال شدید در تولید اسپرم است و پس از تایید مدل آزواسپرمی  به صورت تصادفی به 5 گروه؛ سالم، آزواسپرمی، آزواسپرمی+ورزش، گروه آزواسپرمی+سلول بنیادی و گروه آزواسپرمی+سلول بنیادی+ورزش تقسیم شدند. یک ماه بعد از ایجاد مدل, یک میلیون سلول بنیادی, یک بار به صورت پیوند در ناحیه مجران دفران هر موش پیوند زده شد. تمرین شنا به صورت روزانه به مدت 30 دقیقه در روز و 5 روز در هفته به مدت 8 هفته انجام گرفت. ژن ها با روش Real time-PCR اندازه گیری شد. جهت تجزیه و تحلیل داده ها از آزمون کروسکال والیس استفاده گردید. کلیه محاسبات با استفاده از نرم افزار آماری SPSS/23 و در سطح معنی دار 05/0P≤ انجام شد.
نتایج: نتایج نشان داد بین اثر تمرینات شنا و سلول های بنیادی بر بیان ژن DAZL  (879/22 F=و 0001/0P=) و VASA (359/23 F=و 0001/0P=) در موش های سفید بزرگ آزمایشگاهی مدل آزواسپرمی تفاوت معناداری وجود دارد. همچنین نتایج نشان داد در هر دو متغیر گروه کنترل سالم و گروه آزواسپرمی با تمامی گروه ها اختلاف معنی دار دارندو بین گروه های آزواسپرمی+سلول های بنیادی با آزواسپرمی+تمرین و آزواسپرمی+سلول های بنیادی+تمرین و همچنین گروه آزواسپرمی+تمرین با آزواسپرمی+سلول های بنیادی+تمرین تفاوت معناداری مشاهده نشد.
نتیجه‌گیری: به طور کلی نتایج تحقیق حاضر بیانگر آن است که فعالیت ورزشی منظم هوازی مانند شنا به همراه تزریق سلول های بنیادی در مهار آثار ناشی از بیماری های ناباروری از طریق افزایش بیان ژن DAZL و VASAدر بهبود فرایند اسپرماتوژنز کمک شایانی می کند.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of swimming training and stem cells on the expression of DAZL and VASA genes in azoospermia model rats

نویسندگان [English]

  • Maziyar Shojaee 1
  • Lida Moradi 1
  • Parvin Farzanegi 2
  • Bahram Abedi 2
1 Department of Physical Education and Sports Science, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran
چکیده [English]

Background and Objective: Spermatogenesis is a process that occurs with the proliferation and differentiation of spermatogonial stem cells. These cells are located on the basement membrane of seminiferous tubules and Sertoli cells surround them. This complex provides an environment that causes spermatogenesis to function and survive. The purpose of this research was to investigate the effect of swimming training and stem cells on the expression of DAZL and VASA genes in azoospermia model rats.
Materials and Methods: The research method is experimental in terms of controlling variables and practical in terms of purpose. Twenty-five 8-week-old rats were selected and after the induction of the azoospermia model, which histological studies showed the clear degeneration of spermatogenic cells, which leads to the formation of thin-walled seminiferous epithelium in most of the seminiferous tubules, which indicates a severe disorder in sperm production, and after confirmation Azoospermia model randomly divided into 5 groups; Healthy, azoospermic, azoospermic+exercise, azoospermic+stem cell group and azoospermic+stem cell+exercise group were divided. One month after the creation of the model, one million stem cells were transplanted once in the vas deferens of each mouse. Swimming training was done daily for 30 minutes a day and 5 days a week for 8 weeks. Genes were measured by Real time-PCR method. Kruskal-Wallis test was used to analyze the data. All calculations were done using SPSS/23 statistical software and at a significant level of P≤0.05.
Results: The results showed that there is a difference between the effect of swimming exercises and stem cells on the expression of DAZL (F=22.879 and P=0.0001) and VASA (F=23.359 and P=0.0001) genes in rats. There is a significant difference in the azoospermia model. The results showed that there are significant differences in both variables of the healthy control group and the azoospermic group with all groups, and between the groups of azoospermia+stem cells with azoospermia+exercise and azoospermia+stem cells+exercise as well as the group of azoospermia+exercise with azoospermia+ Stem cells + exercise no significant difference was observed.
Conclusion: In general, the results of the present research indicate that regular aerobic exercise such as swimming along with stem cell injection helps in controlling the effects of infertility diseases by increasing the expression of DAZL and VASA genes in improving the process of spermatogenesis.

کلیدواژه‌ها [English]

  • Azoospermia
  • stem cells
  • swimming training
  • DAZL
  • VASA

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