تاثیر چهار هفته تمرین هوازی با شدت متوسط و مکمل نیگلاپسین (ترکیب روغن سیاه‌دانه و عسل) بر سطح سرمی IgG و IgM در موش صحرایی درمان شده با لیپوپلی ساکارید

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

مقدمه و هدف: با توجه به شیوع بیماری­های ویروسی و التهابات سیستم ایمنی در سراسر دنیا، در جهت بهبود و تقویت عملکرد سیستم ایمنی باید سبک زندگی اصلاح گردد. یکی از بهترین روش­ها استفاده از مکمل­های گیاهی به همراه فعالیت ورزشی می­باشد، که در این مطالعه بررسی شده است.
مواد و روش ها: برای انجام تحقیق آزمایشی حاضر 25 سر موش صحرایی نژاد ویستار از از مرکز سرم­سازی رازی خریداری شده و بطور تصادفی به 5 گروه 5 تایی کنترل- مدل  LPS (lipopolysaccharide)،  تمرین- مدل LPS، مکمل- مدل LPS،  تمرین، مکمل، مدل LPS تقسیم شدند. ایجاد مدل بر روی موش­ها به روش تزریق با ماده LPS با دوز 100 میکروگرم بر کیلوگرم به صورت درون صفاقی به گروه­های مدل بصورت 2 بار در طی 2 هفته انجام شد همچنین گروه­های دیگر به همان میزان دارونما دریافت کردند. سپس گروه­های آزمایشی، به مدت 4 هفته و 3 روز در هفته تمرینات (Moderate Intensity Interval Training) MIT را انجام دادند. همچنین 5 روز در هفته مکمل به صورت گاواژ دریافت کردند. نهایتا داده­ها با استفاده از آزمون تحلیل واریانس یک طرفه و آزمون تعقیبی توکی تجزیه و تحلیل شدند.
نتایج: داده­ها نشان داد تمرین MIT به تنهایی و در تعامل با مکمل موجب کاهش سطح سرمی آنتی­بادی­های IgG و IgM گردیده وتاثیر معنادار دارند (p<0.05).
نتیجه‌گیری: با توجه به نتایج توصیه می شود از تمرینات MIT و مکمل نیگلاپسین (ترکیب سیاهدانه و عسل) جهت تاثیر بر فاکتورهای مهم در سیستم ایمنی استفاده شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of 4-week aerobic training with moderate intensity and niglapsin supplement (combination of black seed oil and honey) on the serum level of IgG and IgM in rats treated with lipopolysaccharide

نویسندگان [English]

  • AmirAbbas BaradaranEsfahani
  • Amir HajiGhasem
  • Forouzan Fattahi Masrour
Department of Sports Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Background and Objective: Due to the prevalence of viral diseases and inflammations of the immune system all over the world and in order to improve and strengthen the function of the immune system, lifestyle should be modified. One of the best methods is the use of herbal supplements along with sports activities which is investigated in this study.
Materials and Methods: To conduct the present experimental research, 25 Wistar male rats were purchased from Razi Serum Center and randomly divided into 5 groups : control- LPS model- exercise, LPS model- supplement, LPS model- exercise, supplement and LPS model. Creating a model on rats was done by injecting LPS at a dose of 100 micrograms/kg intraperitoneally to the model groups received the same amount of placebo. Then the experimental groups performed MIT exercises for 4 weeks and 3 days a week and also received supplements by gavage 5 days a week. Finally, the data were analyzed using one-way analysis of variance and Tukey's post hoc test.
Results: The data showed that MIT training alone and in combination with supplements have a significant effect on IgG and IgM antibodies (p<0.05).
Conclusion: According to the results, it is recommended to use MIT exercises and niglapsin supplement (combination of black seed and honey) to affect important factors in the immune system.

کلیدواژه‌ها [English]

  • Aerobic Exercise
  • Black seed
  • Honey
  • IgG
  • IgM

مراجع

  1. Shimizu K, Sato H, Suga Y, Yamahira S, Toba M, Hamuro K, et al. The effects of Lactobacillus pentosus strain b240 and appropriate physical training on salivary secretory IgA levels in elderly adults with low physical fitness: a randomized, double-blind, placebo-controlled trial. Journal of Clinical Biochemistry and Nutrition 2014;54(1):61-66.
  2. Moreira A, Mortatti AL, Arruda AF, Freitas CG, de Arruda M, Aoki MS. Salivary IgA response and upper respiratory tract infection symptoms during a 21-week competitive season in young soccer players. Journal of Strength and Conditioning Research 2014;28(2):467-73.
  3. Matsuzaki K, Sugimoto N, Islam R, MDE Hossain , Sumiyoshi E, Katakura M, et al. Salivary Immunoglobulin A Secretion and Polymeric Ig Receptor Expression in the Submandibular Glands Are Enhanced in Heat-Acclimated Rats.  International Journal of Molecular Sciences 2020;21(3):815
  4. Laing SJ, Gwynne D, Blackwell J, Williams M, Walters R, Walsh NP. Salivary IgA response to prolonged exercise in a hot environment in trained cyclists. European Journal of Applied Physiology 2005; 93(5-6):665-71.
  5. Mohebbi H, Azizi M, Moradiani H. Effect of 8 Weeks Low and High Intensity Resistance Training on Leukocyte Count, Igg, Cortisol and Lactate Concentration in Untrained Men. World Applied Sciences Journal 2012; 16(7):949-954.
  6. Etemadi H, Nikseresht A. The influence of duration of longdistance road cycling on the amounts of immunoglobulin A & G in blood of professional cyclists. Advances in Environmental Biology 2013; 7(9):2105-10.
  7. Mubarak A, Alturaiki W, Hemida MG. Middle east respiratory syndrome coronavirus (MERS-CoV): infection, immunological response, and vaccine development. Journal of Immunology Research 2019; 6491738.
  8. Lippi G, Salvagno GL, Danese E, Skafidas S, Tarperi C, Cesare Guidi G, et al. Mean platelet volume (MPV) predicts middle distance running performance. PloS One 2014; 9(11): 8-13.
  9. Akbarpour Beni M, Akbari Z, Assarzadeh M, Azizbeigi K. The Effect of Selected Aerobic Training on Serum Immunoglobulin Levels and Testosterone and Cortisol Hormones in Young Men. International Journal of Sport Studies 2013; 3(9):956-62.
  10. Mohebbi H, Azizi M, Moradiani H (2012). Effect of 8 Weeks Low and High Intensity Resistance Training on Leukocyte Count, Igg, Cortisol and Lactate Concentration in Untrained Men. World Applied Sciences Journal 2012; 16 (7): 949-954.
  11. Wang M-Y, An L-G . effect of 12 weeks TAI CHI CHUAN practice on the immune function of female college students who lack physical exercise.biol.sport.2011; 28:45-49.
  12. EL-KADER SMA. Moderate versus high intensity exercise training on leptin and selected iImmune system response in obese subjects. European Journal of General Medicine 2011;8(4):268-272.
  13. Hasani-ranjbar s, jouyandeh z, abdollahim,a systematic review of anti- obesity medicinal plants an update. Journal of diabetes and metabolic disorders.2013;12(1):28.
  14. Estevinho L, Paula Pereira A, Moreira L, G Dias L, Pereira E. Antioxidant and antimicrobial effect of phenolic compounds extracts of northeast Portugal honey. Food and Chemical Toxicology 2008;46(12):3774-3779.
  15. Raju BGS, Battu GR, Latha.Y.B M, Srinivas K. Anti heapto toxic activity of smilax china roots on ccl4 induced hepatic damage in rats . International Journal of Pharmacy and Pharmaceutical Sciences 2012;4:494-6.
  16. Zhong Y, Zhang X, Hu X, Li Y. Effects of Repeated Lipopolysaccharide Treatment on Growth Performance, Immune Organ Index, and Blood Parameters of Sprague-Dawley Rats.  Journal of Veterinary Research 2018;62(3):341-346.
  17. Trochimiak T, Hübner-Woźniak E. Effect of exercise on the level of immunoglobulin a in saliva. Biology of Sport 2012;29(4):255-261.
  18. Mohamed G, Taha M. Comparison between the effects of aerobic and resistive training on immunoglobulins in obese women Bulletin of Faculty of Physical Therapy 2016; 21(1): 11.
  19. Crdova A, Sureda A, Tur J, Pons A. Immune response to exercise in elite sportsmen during the competitive season. Journal of Physiological Biochemistry 2010; 66(1):1-6.
  20. Li TL, Rush B. The Effects of Prolonged Strenuous Exercise on Salivary Secretion of IgA Subclasses in Men. International Journal of Sport and Exercise Science 2009; 1(3):69-74.
  21. Babaei P, Damirchi A, Assarzadeh M. The Effect of a Single Maximal Aerobic Training on Serum IgG and IgA. Journal of Guilan University of Medical Sciences 2003; 12 (46) :1-6
  22. Shirvani H, Ghahreman Tabrizi K, Sobhani V. Effects of high intensity intermittent exercise on serum Immunoglobulin’s and Complement system response in youth soccer players . Journal of Birjand University of Medical Sciences 2013; 20 (3) :233-243.
  23. McKune A, Smith L, Semple S, Wadee A. Influence of ultra-endurance exercise on immunoglobulin isotypes and subclasses. Journal of Sports Medicine 2005; 39(9): 665-70.
  24. Beutler B, Rietschel ET. Innate immune sensing and its roots: the story of endotoxin. Nature reviews. Immunology 2003;3(2):169-76.
  25. Park BS, Song DH, Kim HM, Choi BS, Lee H, Lee JO. The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex. Nature. 2009;458(7242):1191-5.
  26. Kadafi KT, Wibowo S. Differences in systemic humoral immune response among Balb/c mice administered with probiotic, LPS Escherichia coli, and probiotic-LPS E. coli. Iranian Journal of Microbiology 2019;11(4):294-299.
  27. Kasapis C, Thompson PD. The effects of physical activity on serum C-reactive protein and inflammatory markers: a systematic review.  Journal of the American College of Cardiology 2005;45(10):1563-1569.
  28. Meddah B, Ducroc R, El Abbes Faouzi M, Eto B, Mahraoui L, Benhaddou-Andaloussi A, et al. Nigella sativa inhibits intestinal glucose absorption and improves glucose tolerance in rats. Journal of Ethnopharmacology 2009; 121(3): 419-424.
  29. Amr A, Reqz. Effects study of nigella sativa its oil and their combination with vitamin E on oxidative stress in American Journal of Applied Sciences 2014 ;11(7):1079-1086.
  30. Rennie KL, Hughes J, Lang R, Jebb SA. Nutritional management of rheumatoid arthritis: a review of the evidence. J Hum Nutr Diet 2003; 16 97-109.
  31. Karmi, Mohammad; Salarian, Asia; Haji Ghorbanzadeh, Zahra. Evaluating the effectiveness of honey in chronic rheumatoid arthritis. Journal of traditional medicine of Islam and Iran 2011; 3(3):274-269.
  32. Mollazadeh H, Hosseinzadeh H. The protective effect of Nigella sativa against liver injury: a review . Iranian Journal of Basic Medical Sciences 2014;17(12):958-66.
دانشور پزشکی
دوره 32، شماره 2 - شماره پیاپی 171
خرداد و تیر 1403
صفحه 1-9

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