دانشور پزشکی

دانشور پزشکی

اثر نانو ذرات طلای سنتز شده توسط میکرو جلبک اسپیرولینا پلاتنسیس بر شاخص‌های استرس اکسیداتیو در موش های سفید بزرگ آزمایشگاهی تغذیه‌شده با غذای پرچرب

نوع مقاله : مقاله پژوهشی

نویسندگان
حامد عبداله اصلیان 1
محمدرضا طاهری زاده 1
محمد رفیعی نیا 2
پرهام رئیسی 3
الهام بیدرام 2
1 گروه زیست دریا، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران
2 گروه بیومتریال، نانوتکنولوژی و مهندسی بافت، دانشکده فناوری‌های نوین علوم پزشکی، دانشگاه علوم پزشکی اصفهان، ایران
3 گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، ایران
10.22070/daneshmed.2024.17991.1380
چکیده
مقدمه و هدف: با توجه به اهمیت نانو ذرات طلا و میکروجلبک اسپیرولینا در داروسازی، بررسی تأثیر آن‌ها در حیطه‌های مختلف زیست پزشکی بسیار ضروری می‌نماید. این تحقیق با هدف بررسی اثر  نانو ذرات طلای سنتز شده توسط میکرو جلبک اسپیرولینا پلاتنسیس بر شاخص‌های استرس اکسیداتیو در موش های سفید بزرگ آزمایشگاهی تغذیه‌شده با غذای پرچرب، صورت گرفته است.
مواد و روش ها: به وسیله عصاره آبی اسپیرولینا پلاتنسیس، بیوسنتز نانو ذرات طلا صورت پذیرفت. در ادامه آزمایش، 30 موش های سفید بزرگ آزمایشگاهی  به پنج گروه تقسیم گردید که شامل گروه کنترل که با غذای پلت استاندارد تغذیه شدند، گروه مدل که با رژیم غذایی پرچرب و بدون هیچ‌گونه درمانی تغذیه شدند و سه گروه آزمایشی دیگر که همراه با رژیم غذایی پرچرب از نانو ذرات بیوسنتز شده در دو دوز بالا و پایین و گروهی که فقط با میکرو جلبک اسپیرولینا تغذیه ‌شدند.
نتایج: طبق نتایج، در طول‌موج 540 نانومتر و در دمای 40 درجه سانتی‌گراد، سنتز بهینه به ثبت رسید و شکل نانو ذرات کروی بوده است. این مطالعه افزایش قابل‌توجهی را در میزان SOD، GPx، و CAT در گروه‌های HFD+The Au-Sp (دوز بالا) و HFD+Spirulina در مقایسه با گروه مدل نشان داد. میزان مالون دی آلدهید در هر دو گروه HFD+The Au-Sp (دوز بالا) و HFD+Spirulina در مقایسه با گروه مدل به‌طور قابل‌توجهی کاهش یافت.
نتیجه‌گیری: نتایج بیانگر این است که عصاره اسپیرولینا پلاتنسیس، منبع زیستی مناسبی برای سنتز نانو ذرات طلا است و این محصول را برای مصارف پزشکی در موقعیت ایده آل قرار می‌دهد.
کلیدواژه‌ها
نانو ذرات طلا
میکرو جلبک دریایی
بیوسنتز
آنزیم های اکسیداتیو
کاتالاز
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دانشور پزشکی
دوره 32، شماره 1 - شماره پیاپی 170
فروردین و اردیبهشت 1403
صفحه 1-16