نوع مقاله : مقاله پژوهشی
1 گروه ایمونولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران
2 مرکز تحقیقات تنطیم پاسخهای ایمنی، دانشگاه شاهد، تهران، ایران
3 مرکز تحقیقات بیماری های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران
عنوان مقاله [English]
Background and Objective: Covid-19 disease as a pandemic with high spreading power and a wide range of clinical symptoms and lack of definitive treatment is a challenge for mankind in recent years. The antiviral response of type 1 interferon against the SARS-COV-2 virus is one of the most important pathways of the innate immune system in dealing with this disease. The purpose of this study was to investigate the relationship between the expression of two genes, ly6e and usp18, with the severity of clinical symptoms in patients with covid-19.
Materials and Methods: Peripheral blood samples were prepared from patients and their DNA was extracted, then the expression of ly6e and usp18 genes was measured by real-time PCR method. cDNA samples were first amplified using specific primers. Data analysis was performed using chi-square, Kruskal-Wallis and Mann-Whitney tests. A significance level of 0.05 was considered.
Results: The expression of the ly6e gene was significantly (p=0.008) decreased in the hospitalized group compared to the control group. No significant difference was seen in the expression of the usp18 gene among the studied groups.
Conclusion: Proper expression of ly6e protein can be related to controlling the severity of clinical symptoms of covid-19 disease.