اثر تمرین تناوبی شدید (HIIT) همراه با مهار پپتید وابسته به کلسی تونین (CGRP) بر بیان ژن فاکتور رشد اپیدرمی (EGF) و گیرنده فاکتور رشد اپیدرمی (EGFR) در بافت هیپوکمپ موش‌های نر نژاد ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده روان شناسی و علوم تربیتی،گروه علوم ورزشی،دانشگاه سیستان و بلوچستان،زاهدان ،ایران

2 دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران

چکیده

مقدمه و هدف: فاکتورهای رشد، بیوملکول های پپتیدی کوچکی اند که نقش مهمی در افزایش بیماری های خود ایمنی دارند ودر پاتوژنز بسیاری از انواع سرطان ها دخیل هستند. هدف اصلی این مطالعه بررسی تأثیر 6 هفته تمرین تناوبی با شدت بالا (HIIT) همراه با مهار پپتید مرتبط با ژن کلسی‌تونین (CGRP) بر بیان ژن فاکتور رشد اپیدرمی (EGF) و گیرنده فاکتور رشد اپیدرمی (EGFR) در بافت هیپوکمپ موش های نر نژاد ویستار بود.
مواد و روش ها: در این مطالعه تجربی 32 سر موش بزرگ آزمایشگاهی نر نژاد ویستار با میانگین وزن 15±205 گرم و سن 8 هفته طور تصادفی در 4 گروه 8 سری شامل: 1) کنترل (C)، 2) مهار CGRP(CI)، 3) HIIT(TH) و 4) مهار CGRP+HIIT(CI+TH) تقسیم شدند. جهت مهار CGRP در گروه 2 و 4 از آنتی بادی CGRP با دوز 25/0 میلی گرم بر کیلوگرم به صورت درون صفاقی استفاده شد. پروتکل تمرین HIIT به مدت 6 هفته و 5 روز در هر هفته انجام شد، که به صورت 10 وهله دویدن شدید 2 دقیقه­ای روی تردمیل و 1 دقیقه دویدن آهسته ( استراحت فعال) به صورت فزاینده بود. در نهایت بافت مورد نظر استخراج و میزان بیان ژن EGF و EGFR به وسیله روش Real time–PCR ΔΔ و با استفاده از فرمولΔΔCt 2، مورد ارزیابی قرار گرفت جهت تجزیه و تحلیل یافته­ها از آزمون آنالیز واریانس یک­راهه به همراه آزمون تعقیبی توکی در نرم‌افزار SPSS نسخه 26 استفاده شد (05/0≥p).
نتایج: پژوهش حاضر نشان داد سطوح EGF بافت هیپوکمپ در گروه (TH) به طور معناداری بالاتر از گروه های (CI) (001/0=P) و گروه (C) (006/0=P) بود. همچنین در گروه (CI+TH)  به طور معناداری بالاتر از گروه (CI) (007/0=P) بود. سطوح بیان ژنی EGFR در گروه (CI+TH) به طور معناداری بالاتر از (CI) (04/0=P) و (TH) (01/0=P) بود.
نتیجه‌گیری: تغییرات فیزیولوژیکی ناشی از HIIT در هیپوکمپ می تواند وضعیت عملکردی مغز را از طریق تنظیم مثبت بیان ژن EGF و EGFR به عنوان یک روش غیردارویی موثر بهبود بخشد. علاوه بر این، مهار HIIT + CGRP ممکن است یک رویکرد جدید را از طریق مسیرهای EGF و EGFR در هیپوکمپ نشان دهد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of HIIT and CGRP inhibition on EGF and EGFR gene expression in the hippocampal tissue of male Wistar rats

نویسندگان [English]

  • Asma Jafari Khooshan Abadi 1
  • Sajjad Ramezani 2
  • Shila Nayebifar 1
1 Faculty of Psychology and Educational Sciences, Department of Sports Sciences, University of Sistan and Baluchistan, Zahedan, Iran
2 Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

Background and Objective: Growth factors are small peptide biomolecules that play an important role in increasing autoimmune diseases and are involved in the pathogenesis of many types of cancer. The main purpose of this study is to investigate the effect of 6 weeks of HIIT with inhibition of CGRP on EGF and EGFR gene expression in the hippocampal tissue of male Wistar rats.
Materials and Methods: In this experimental study, 32 Wistar male laboratory rats with an average weight of 205±15 grams and an age of 8 weeks were randomly divided into 4 groups of 8 series including         1-control (C), 2-inhibition of CGRP (CI), 3-HIIT (TH). ) and               4-inhibition of CGRP+HIIT (CI+TH) were divided. In order to inhibit CGRP in groups 2 and 4, CGRP antibody was used intraperitoneally at a dose of 0.25 mg/kg. The HIIT training protocol was performed for 6 weeks and 5 days per week, which consisted of 10 bouts of intense       2-minute running. on the treadmill and 1 minute of slow running (active rest) was incremental. Finally, the desired tissue was extracted and the expression level of EGF and EGFR genes was evaluated by Real time-PCRΔΔ method and using 2 ΔΔCt formula. To analyze the findings, one-way analysis of variance was used along with Tukey's post hoc test in SPSS software version 26 (p≤0.05).
Results: The results of the present study showed that the levels of EGF in hippocampal tissue in group (TH) were significantly higher than groups (CI) (P=0.001) and (C) (P=0.006). It was also significantly higher in the (CI+TH) group than the (CI) group (P=0.007). EGFR gene expression levels in   (CI+TH) group were significantly higher than (CI) (P=0.04) and (TH) (P=0.01).
Conclusion: Physiological changes caused by HIIT in the hippocampus can improve the functional state of the brain through positive regulation of EGF and EGFR gene expression as an effective non-pharmacological method. Furthermore, HIIT+CGRP inhibition may represent a novel approach through the EGF and EGFR pathways in the hippocampus.

کلیدواژه‌ها [English]

  • High-Intensity Interval Trainings (HIIT)
  • Epidermal Growth Factor (EGF)
  • Calcitonin Gene-Related Peptide (CGRP)
  • Hippocampus

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دانشور پزشکی
دوره 30، شماره 6 - شماره پیاپی 163
بهمن و اسفند 1401
صفحه 10-19

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