اثر تمرین مقاومتی همراه با ویتامینD با پوشش کیتوزان بر بیان ژن هایSemaphorine-a3 وNLRP-1 پیوستگاه عصبی عضله چهار سر ران رت های یائسه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه فیزیولوژی ورزشی، واحد مهاباد، دانشگاه آزاد اسلامی، مهاباد، ایران

3 گروه تربیت‌بدنی، واحد پردیس، دانشگاه آزاد اسلامی، پردیس، ایران

چکیده

زمینه و هدف: فرآیند یائسگی باعث انحطاط نورون‌های عصب حرکتی تارهای تند انقباض می‌شود. اما اثر تمرین مقاومتی همراه با مکمل ویتامینD ترکیبی بر مهار ژن‌های موثر درآتروفی به خوبی مشخص نیست. بر این اساس هدف از مطالعه حاضر بررسی اثر 8 هفته تمرین مقاومتی همراه با ویتامینD با پوشش کیتوزان بر بیان ژن‌های Semaphorine-a3 وNLRP-1 پیوستگاه عصبی عضله چهار سر ران رتهای یائسه بود.
روش بررسی: در یک کارآزمایی پیش بالینی، 42 سر موش صحرائی ماده نژاد ویستار 8 تا 12 هفته‌ای، با میانگین وزن 230 تا 260 گرم، به طور تصادفی به 6 گروه 7 تایی: کنترل سالم (NC)، کنترل یائسه (OC)، استروژن (E)، تمرین مقاومتی (RT)، ویتامینD با کیتوزان(Vit D+Ca+2)، تمرین مقاومتی+ ویتامینD با کیتوزان(Ca+2+RT+Vit D) تقسیم شدند. پس از اوارکتومی، گروه تمرین مقاومتی 5 روز در هفته به مدت 8 هفته تمرین خود را انجام دادند. ویتامین D با کیتوزان در دوز 100 میلی‌گرم بر کیلوگرم قبل از تمرین گاواژ شد. جهت تعیین بیان ژن‌های Semaphorin-a3 و NLRP-1 خارج شد.
یافته‌ها: بیان ژنSemaphorine-a3 در گروه تمرین مقاومتی+ ویتامینD با کیتوزان نسبت به گروه کنترل یائسه(001/0P=) و گروه تمرین (002/0P=)کاهش معناداری داشت. بیان ژن NLRP-1 در گروه تمرین مقاومتی+ ویتامینD با کیتوزان نسبت به گروه تمرین کاهش معناداری داشت (043/0P=).
نتیجه‌گیری: تمرین مقاومتی باعث کاهش بیان ژن‌هایSemaphorine-a3 وNLRP-1 شد و ویتامینD با پوشش کیتوزان اثر تمرین مقاومتی را بر کاهش بیان ژن NLRP-1 افزایش داده و احتمالا می‌تواند عملکرد عصبی- عضلانی را در افراد یائسه بهبود دهد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of resistance training and vitamin D with chitosan coating on Semaphorine-a3 and NLRP-1 genes of neural junction in quadriceps muscle of ovariectomized rats

نویسندگان [English]

  • Tahereh Shadpour Alizadeh 1
  • Mohammad Ali Azarbayjani 1
  • Sirvan Atashak 2
  • Maghsoud Peeri 1
  • Saleh Rahmati Ahmad Abad 3
1 Department of Exercise Physiology , Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Exercise Physiology , Mahabad Branch, Islamic Azad University, Mahabad, Iran
3 Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran
چکیده [English]

Background and Objective: The menopausal process causes degeneration of motor nerve neurons in fast-twitch fibers. However, the effect of resistance training with vitamin D supplementation on the inhibition of genes involved in atrophy is not well understood. The purpose of this study was to determine the effect of resistance training and vitamin D with chitosan coating on Semaphorine-a3 and NLRP-1genes of neural junction in quadriceps muscle of postmenopausal rats.
Materials and Methods: In this experimental study, 42 femal of wistare rats with ages 8 to 12 weeks with an average weight of 230 to 260 g were randomly divided into 6 groups of 7 numbers; normal control (NC), menopause control (OC), estrogen (E), resistance training (RT),vitamin D white chitosan (Vit D+Ca+2), resistance training+Vitamin D with chitosan (RT+Vit D+Ca+2). Training group performed resistance training five days a week for eight weeks. Vitamin D with chitosan was gavage at a dose of 100 mg/kg before exercise. PCR Real time was used to determine the expression of Semaphorine-a3 and NLRP-1genes.
Results: The gene of Semaphorine-a3 in the resistance training group+vitamin D with chitosan compared to the postmenopausal control group and the training group had a significant decrease (P=0.001) and (P=0.002), respectively.  NLRP-1 gene in the resistance training group+vitamin D with chitosan was significantly reduced compared to the training group (P=0.043).
Conclusion: The results showed that resistance training decreased the  genes expression of Semaphorine-a3 and NLRP-1. Decreased expression of NLRP-1 gene under the influence of resistance training combined with vitamin D with chitosan coating could possibly improve neuromuscular function in postmenopausal people.

کلیدواژه‌ها [English]

  • Resistance training
  • Vitamin D with chitosan
  • Menopause
  • Semaphorine-a3
  • NLRP-1

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دانشور پزشکی
دوره 30، شماره 6 - شماره پیاپی 163
بهمن و اسفند 1401
صفحه 32-43

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