مقایسه اثر ضددرد صمغ کتیرا با مورفین و دیکلو فناک در موش سفیدکوچک آزمایشگاهی با استفاده از آزمون‌های قدکشیدن و صفحه داغ

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه آزاد اسلامی واحد علوم و تحقیقات، گروه زیست‌شناسی، فارس، ایران

2 گروه فیزیولوژی، دانشگاه علوم پزشکی شهید صدوقی یزد، ایران

چکیده

مقدمه و هدف: یکی از داروهای گیاهی برای کنترل درد در طب سنتی، صمغ کتیرا بوده­است. هدف از این پژوهش، بررسی اثر ضددردی صمغ کتیرا است.
 مواد و روش ها: در این مطالعه، اثر  ضددردی صمغ کتیرا به­وسیله آزمون­های صفحه داغ Hotplate) ( و قدکشیدن)  (writhing  بررسی­شد.  موش­های سوری نر در سه گروه دریافت­کننده کتیرا با دوزهای 125، 250 و 500 میکروگرم به­ازای هر کیلوگرم وزن، سه گروه دریافت­کننده مرفین با دوزهای 2، 4 و8 میلی­گرم و سه گروه دریافت­کننده دیکلوفناک با دوزهای 10،20 و30 میلی­گرم به­ازای هر کیلوگرم وزن تقسیم­شدند.
 نتایج: درآزمون قدکشیدن دوزهای 125، 250 و 500 میکروگرم بر کیلوگرم صمغ کتیرا باعث کاهش تعداد انقباض­های شکمی و افزایش درصد مهار درد نسبت­به گروه کنترل و در دوز 500 نسبت­به دیکلوفناک mg/kg10 شد (05/0> (P. با بررسی زمان واکنش حیوانات به درد در آزمون صفحه داغ، طی دوره­های زمانی 15،30، 45 و 60  دقیقه، تنها در 15دقیقه پس از تزریق صمغ کتیرا، دوزهای  μg/kg 125 و  μg/kg 500 درد را کاهش­دادند. در 15 دقیقه پس از تزریق، کتیرای   μg/kg125 نسبت­به گروه­های کنترل، دیکلوفناک mg/kg 30، مرفین  mg/kg 2 و4 تفاوتی معنی­دار داشته­است  (001/0 P<).
 نتیجه‌گیری: در این پژوهش، اثر ضددردی قابل­ملاحظه­ای از صمغ کتیرا در آزمون‌های صفحه داغ و قدکشیدن مشاهده­شد که نشان­می­دهد، ترکیب­های تشکیل­دهنده این صمغ دارای آثار ضددرد حاد و مزمن هستند

کلیدواژه‌ها

عنوان مقاله [English]

A comparison between the analgesic effect of tragacanth gum, diclofenac and morphine in mice using writhing and hot-plate analgesic tests

نویسندگان [English]

  • Leila Keihani 1
  • Mohammad Ebrahim Rezvani 2
  • Mohammad Hossain Dashti- Rahmatabadi 2
1 Science and Research Branch, Islamic Azad University, Fars, Iran
2 Shahid Sadoughi University of Medical Sciences, Yazd, Iran
چکیده [English]

Background and Objective: Some of medicinal plants have been used for pain reliving in Iarnian ancient medicine. Astragalus gummifer (AG) is one of them and its gum (tragacanth gum) were used for several health purposes. The present study was conducted to evaluate the analgesic effects of this gum in mice.
Materials and Methods: In this study, the analgesic effect of tragacanth gum was determined using hot-plate and writhing tests. Mice were injected with tragacanth gum at doses of 125, 250 or 500 µg/kg i.p. as treatment groups. Morphine at doses of 2, 4 or 8 µg/kg i.p., and diclofenac at doses of 10, 20 or 30 µg/kg i.p. were used as control groups.
Results: In writhing test, gum tragacanth at different doses (125, 250, and 500 µg/kg ) significantly reduced the number of writhings in mice as compared to control group (p<0.05). In hot-plate test, maximum possible effect of tragacanth gum significantly increased only after 15 minutes but not in other time periods. In two models of pain assesment, both morphine sulfate and diclofenac sodium had also pain relieving potential.
Coclusion: The present study indicated that tragacanth gum elicits prominent analgesic effects in an experimental model of acute and chronic pain. Additionally, data obtained in this study indicated the presence of some constituents with pain releiving properties that confirms the traditional use of the gum for pain relieving purposes.

کلیدواژه‌ها [English]

  • Tragacanth gum
  • Diclofenac
  • Morphine
  • Analgesia
  • Writhing test
  • Hot plate test

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