اثر استیل ال کارنیتین بر فراساختمان سیناپس نورون حرکتی ضایعه دیده موش صحرائی بالغ

نویسندگان

1 دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

2 گروه علوم تشریح و پاتولوژی، دانشکده پزشکی، دانشگاه شاهد ، تهران، ایران

چکیده

مقدمه و هدف: ضایعه فشاری طناب نخاعی عارضه نسبتاً شایع جوامع در حال رشد است.
مواد و روش ها: 16 سر موش صحرایی بالغ ضایعه نخاعی دیده با وزن 250 تا 300 گرم از نژاد اسپراگوداولی در4 گروه بصورت تصادفی قرار گرفتند، گروه ها شامل: الف) لامینکتومی با تزریق روزانه داخل صفاقی استیل ال کارنیتین ب) لامینکتومی با تزریق روزانه داخل صفاقی سرم فیزیولوژی ج) گروه الف + اعمال فشار مکانیکی بر نخاع و د) گروه ب + اعمال فشار مکانیکی بر نخاع. پس از 4 هفته موشها کشته و نمونه بافت نخاعی شان جهت مورفومتری با میکروسکوپ نوری و فراساختاری بوسیله میکروسکوپ الکترونی مطالعه شدند. داده ها ی بدست آمده به وسیله آزمون آماری آنوا و در ادامه آزمون توکی با نرم افزار SPSS Ver 19 مورد بررسی فرار گرفت. سطح معنی داری 05/0 ≥ p < /span> در نظر گرفته شد.
نتایج: بررسی مورفولوژی کاهش تعداد سلول های عصبی حرکتی را بدنبال فشار مکانیکی نشان داد. استیل ال‌کارنیتین مرگ سلول‌های عصبی را کمتر کرد و به حفظ فراساختمان سلولهای عصبی حرکتی در محل سیناپسها با حفظ تعداد و شکل میتوکندری ها کمک کرد.
بحث و نتیجه گیری: در این تحقیق استیل ال کارنتین بر بهبود فراساختمان سیناپس نورون حرکتی پس از ضایعه فشاری موثر واقع شد. همچنین این ماده برحفظ و افزایش سلول‌های عصبی حرکتی و کاهش مرگ آنها اثر کرد. البته میزان تأثیر این تغییر در بهبود ضایعه نخاعی ناشی از فشار مکانیکی نیازمند بررسی های بیشتر است.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of acetyl l-carnitine on ultrastructure of injured motoneuron synapses in adult rat

نویسندگان [English]

  • Mehran Jamali 1
  • Marjan Heshmati 2
  • Mohammadreza Jalali Nadoushan 2
چکیده [English]

Background and Objective: Spinal cord compression is a relatively common neurological complication in developing country. This study was designed to assess neuroprotective effect of acetyl L-carnitine.
Materials and Methods: 16 adult Sprague Dawley rats weighing 250 to 300 g were divided into 4 randomized groups, namely, A-laminectomy with daily intraperitoneal injection of acetyl L-carnitine. B- laminectomy with daily intraperitoneal injection of saline. C- group A+ mechanical compression and D- group B+ mechanical compression. After 4 weeks, they were sacrificed for morphological study by light microscope and ultrastructure of synaptic zone of motoneurons was evaluated by electron microscope. The results were analyzed by Tukey’s and ANOVA by SPSS Ver 19. Furthermore, the significance level was P<0.05.
Results: The morphological results indicated that compression causes reduction of spinal cord motoneurons. Acetyl L-carnitine decreased this reduction of motoneurons after spinal cord compression and preserved motoneurons ultrastructure in synaptic zone and mitochondria.
Conclusion: In this study, acetyl L-carnitine was effective on preservation of ultrastructure of motoneuron synapses after mechanical compression in spinal cord. Of course, the measure of these changes in means of decreasing the effect of mechanical compression after spinal cord compression needs further researches.

کلیدواژه‌ها [English]

  • Acetyl L-carnitine
  • Spinal cord injury
  • synapse ultrastructure
  • Adult Rat
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