تاثیر تمرین هوازی بر بیان پروتئین های Spred-1،Raf1 و VEGF بافت قلب در موشهای صحرایی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده ی تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

2 گروه تربیت بدنی دانشگاه علوم انتظامی امین

چکیده

مقدمه و هدف: دیابت یکی از بیماری­های متابولیکی است که با دیسفانکشن و آسیب عروقی همراه است. تمرینات ورزشی در بیماران دیابتی باعث بهبود رگ زایی می­شود که به روند درمان این بیماران کمک می­کند. هدف پژوهش حاضر، بررسی تمرین هوازی بر بیان پروتئین Spred-1، Raf1 و VEGF بافت قلبی موش‌های صحرایی دیابتی بود.
 
مواد و روش­ها: در این مطالعه، 50 موش صحرایی به صورت رندمایز به چهار گروه دو گروه تمرین هوازی دیابتی و سالم (به مدت 8 هفته و 6 جلسه در هفته) و دو گروه کنترل دیابتی و سالم تقسیم شدند. 48 ساعت پس از اتمام دوره تمرینی عضله قلبی آن­ها تحت شرایط استریل جدا شد. بیان پروتئین VEGF، Spred-1 و Raf-1 با روش الایزا سنجیده شد. برای تجزیه و تحلیل اطلاعات از آزمون تحلیل واریانس یک‌سویه و آزمون تعقیبی توکی در سطح معنی‌داری p<0.05 و نرم‌افزار Spss نسخه 16 استفاده شد.
 
نتایج: نتایج مطالعه نشان داد دیابت موجب کاهش معنی‌دار میزان بیان پروتئین VEGF (p<0.05) و Raf-1 (p<0.05) و افزایش معنی‌دار پروتئین Spred-1 (p<0.05) می‌شود. هم‌چنین، نشان داده شد هشت هفته تمرین هوازی باعث افزایش معنی‌دار بیان پروتئین VEGF (p<0.05) و Raf-1 (p<0.05) و کاهش معنی‌دار پروتئین Spred-1 (p<0.05) می‌شود.
 
نتیجه‌گیری: تمرین هوازی توانست سبب افزایش بیان پروتئین VEGF و Raf1 و کاهش Spred-1 در سلول‌های قلبی شده و به‌این‌ترتیب اثرات دیابت بر بیان این پروتئین‌ها در قلب را بهبود بخشید.
 

کلیدواژه‌ها


عنوان مقاله [English]

The effect of aerobic exercise on protein expression of VEGF, Spred 1, and Raf1 in cardiac tissue of rats

نویسندگان [English]

  • Sajjad Mohammadyari 1
  • Siroos Choobineh 1
  • Rahman Soori 1
  • Abbasali Gaeini 1
  • Hamdolah Hadi 2
چکیده [English]

Background and Objective: Diabetes is a metabolic disorder that is associated with dysfunction and impairment of vascular system. Exercise training in diabetic patients improves angiogenesis, which helps treatment of diabetes. The purpose of this study was investigation of aerobic exercise effect on cardiac expression of the proteins Spred-1, Raf1, and VEGF in diabetic rats.
 
Materials and Methods: In this study, 50 rats were randomly divided into 4 groups of diabetic, intact aerobic exercise (8 weeks for 6 sessions per week), diabetic, and intact control. Cardiac muscle was removed and placed immediately in liquid nitrogen. Protein expression of VEGF, Raf-1 and Spred-1 was investigated by ELISA method. Data were analysed using SPSS (version 16.0) at a significance level of p < 0.05. One way ANOVA was used for data analysis with Tukey’s test to find differences between groups.
 
Result: Results showed that diabetes leads to decreased cardiac expression of Raf-1 (p<0.05) and VEGF (p<0.05) proteins and increased cardiac expression of Spred-1 protein (p<0.05). As well, eight weeks of aerobic training led to increase of cardiac expression of Raf-1 (p<0.05) and VEGF (p<0.05) protein and decreased cardiac expression of Spred-1(p<0.05) protein in diabetic rats.
 
Conclusion: Aerobic exercise training increases expression of VEGF and Raf1 protein and decreases expression of Spred-1 in cardiomyocytes, therefore, the effect of diabetes on these proteins in the presence of aerobic exercise is attenuated.
 

کلیدواژه‌ها [English]

  • Aerobic training
  • Vascular endothelial growth factor
  • Diabetes
  • spred-1
  • Raf1
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