نویسندگان
1 مرکز تحقیقات علوم اعصاب ، دانشگاه علوم پزشکی بقیه ا...(عج)،تهران
2 پژوهشکده فناوری های زیستی،دانشگاه صنعتی مالک اشتر
3 مرکز تحقیقات علوم اعصاب و گروه آناتومی ، دانشگاه علوم پزشکی بقیه ا...(عج)
4 مرکز تحقیقات نانوبیوتکنولوژی ، دانشگاه علوم پزشکی بقیه ا...(عج)
5 مرکز تحقیقات علوم اعصاب ، دانشگاه علوم پزشکی بقیه ا...(عج)
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Background and Objective: Several studies have been performed to achieve a scaffold for growing stem cells.
The purpose of the study was to provide a biodegradable scaffold of chitosan -
poly ethylene oxide (PEO) with the ability for growing, proliferation, un-differentiation
and apoptosis of bone marrow stromal cells (BMSCs).
Materials
and Methods: First,
formation of chitosan-PEO nanofibers
composed of 90 to 10 and 80 to 20 per electro technique were studied by scanning
electron microscope (SEM). These scaffolds were located on 1% gelatin in
24-well plates and were then steriled. Femoral BMSCs of rats were
cultured on scaffolds after two passages from the house empty plate as
controls. BMSCs proliferation, differentiation and apoptosis were
studied in days II, IV and VI. Results: The results showed that the morphology of cells was maintained on scaffolds similar
to controls. The rate of cell proliferation on the scaffold on consecutive days increased in cultured cells of control group < /span> but the differences were not significant. The results also showed that at the end of the six days, BMSCs differentiation and the percentage of cell death on the scaffold were similar with cultured cells in control group.
Conclusion: P < /span>roliferation, un-differentiation and no
apoptosis of BMSCs on biodegradable chitosan-PEO nanofiber are obtained as a model that can be used in tissue engineering and cell therapy
کلیدواژه‌ها [English]
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