نویسندگان
1 گروه ایمونولوژی، دانشکده پزشکی، دانشگاه شاهد
2 گروه بیوشیمی، مرکز تحقیقات بیوشیمی بیوفیزیک دانشگاه تهران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Background and Objective: Proliferation and apoptosis of
lymphocytes are essential parts of the immune system. Most anti-cancer
chemotherapy drugs such as vincristine target cell cycle and induce apoptosis
in cancer cells. In other words,
dividing cells undergo more apoptosis, which may also include the normal
proliferating lymphocytes responsive to malignancies as well. Materials and Methods: In this study, the effect of different concentration of
vincristine at three different time periods on resting and proliferating spleen
lymphocytes were evaluated
and compared with the effect of the drug on mouse lymphoma cell line BCL1. The cytotoxicity of vincristine
was determined by MTT assay and IC50 was calculated for all periods. The cells
were also stained with double staining acridine orange and ethidium bromide and
were observed with fluorescence microscope and the percentage of apoptotic
cells were determined. Results: MTT
results showed that vincristine at concentrations of 10 and 20 μg/ml caused
cell death in both resting and proliferating lymphocytes, but
concentrations
کلیدواژهها [English]
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