نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، گروه فیزیولوژی ورزشی، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران
2 گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران
3 آموزگار تربیتبدنی، آموزشوپرورش ناحیه دو اهواز، استان خوزستان، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Background and Objective: Chronic inflammation caused by obesity is one of the key factors in the development of metabolic disorders. The HMGB1-RAGE signaling pathway is recognized as a crucial molecular axis in the initiation and persistence of metabolic inflammation in visceral adipose tissue. This study aimed to investigate the effects of aerobic exercise and probiotic supplementation on the gene expression levels of HMGB1 and RAGE in the visceral adipose tissue of obese male Wistar rats.
Materials and Methods: In this experimental study, 40 male Wistar rats were divided into two groups: standard diet and high-fat diet containing fructose. After 24 weeks of obesity induction, they were assigned to five subgroups: control, obese, obese + exercise, obese + probiotic, and obese + exercise + probiotic. Aerobic exercise was performed on a treadmill for 8 weeks, and probiotics were administered orally. At the end of the study, gene expression levels of HMGB1 and RAGE in visceral adipose tissue were measured using Real-Time PCR.
Results: The results showed that the high-fat and fructose diet significantly increased the expression of HMGB1 and RAGE genes compared to the healthy group (P<0.001). In contrast, aerobic exercise, probiotic supplementation, and especially their combination significantly reduced the expression of these genes (P<0.05). Additionally, anthropometric indices and lipid profiles improved in the intervention groups.
Conclusion: Aerobic exercise and probiotics, particularly when combined, can reduce obesity-induced chronic inflammation by inhibiting the HMGB1-RAGE inflammatory pathway in visceral adipose tissue. These findings emphasize the effectiveness of non-pharmacological lifestyle interventions in preventing and managing obesity-related metabolic complications.
کلیدواژهها [English]
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