دانشور پزشکی

دانشور پزشکی

تاثیر تمرین هوازی و رزوراترول بر بیان ژنی SIRT1 و TNF-α در تخمدان موش های مبتلا به پلی کیستیک تخمدان

نوع مقاله : مقاله پژوهشی

نویسندگان
معصومه حبیبیان 1
سوفیا عالی 2
1 دانشگاه آزاد قائم شهر
2 گروه تربیت بدنی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران
10.22070/daneshmed.2025.20455.1606
چکیده
مقدمه و هدف: چاقی و کم‌تحرکی منجر به التهاب مزمن با درجه پایین می‌شود که با اختلال عملکرد تخمدان همراه است. التهاب مزمن نقش مهمی در پاتوژنز سندرم تخمدان پلی‎کیستیک‌(PCOS) دارد. هدف از مطالعه حاضر بررسی تاثیر تمرین هوازی و رزوراترول بر بیان ژنی سیرتوئین1(SIRT1)و فاکتور نکروز دهنده تومور-آلفا‎(TNF-α) در تخمدان موش‌های مبتلا به PCOS می‌باشد.
مواد و روش‌ها: در این مطالعه 30 سر موش ماده گونه C57bl/6، 8 هفته‌ای بصورت تصادفی به گروه‌های کنترل-سالم، کنترل-بیمار، بیمار-تمرین، بیمار-رزوراترول و بیمار-تمرین-رزوراترول تقسیم‌بندی شدند. PCOS، با گاواژ 60 میلی‌گرم/‌کیلوگرم دی‌هیدرو اپی‌آندرستندیون به مدت 20 روز القا شد. رزوراتول روزانه با دوز 75/3 میلی‌گرم/کیلوگرم به مدت 8 هفته تزریق درون‌صفاقی شد. برنامه تمرینی شامل دویدن روی نوارگردان با سرعت9 متر بر دقیقه به مدت 25 دقیقه در هفته اول با شیب 1 درجه بود که با افزایش تدریجی به سرعت 22 متر/دقیقه به مدت 55 دقیقه با شیب 8 درجه در هفته هشتم رسید. آنالیز داده‌ها از طریق آزمون‌های آماری تحلیل واریانس یک‌طرفه و تعقیبی‌توکی انجام شد(05/0>P).
نتایج: با القای PCOS، بیان ژنی TNF-α افزایش معنادار و بیان ژنی SIRT1 کاهش معناداری در بافت تخمدان یافت(000/0>P). هر دو مداخله تمرین‌هوازی و رزوراتول منجر به تنظیم‌منفی بیان ژنی TNF-α و افزایش بیان ژنی SIRT1 شد اما تاثیر مداخله ترکیبی بر این تغییرات در مقایسه با دو مداخله دیگر بیشتر بود(000/0>P).
نتیجه گیری: به نظر می‌رسد که هر دو مداخله تمرین‌هوازی و مکمل رزوراتول می‌تواند از بافت تخمدان در برابر آسیب ناشی از PCOS ، بواسطه تنظیم‌منفی بیان ژنی TNF-α و افزایش SIRT1 حفاظت کند.
کلیدواژه‌ها
SIRT1
فاکتور نکروز دهنده تومور-α
التهاب
سندرم تخمدان پلی کیستیک
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دانشور پزشکی
دوره 33، شماره 2 - شماره پیاپی 177
خرداد و تیر 1404
صفحه 23-32