اثر شش هفته تمرین هوازی فزاینده بر سطوح پروتئین های PRDM16 و PPAR-y در بافت چربی موش‌های دیابتی شده با استرپتوزوسین The effect of six weeks of incremental aerobic training on PRDM16 and PPAR-y protein levels in adipose tissue of streptozotocin-induced diabetic rats

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزشی، گروه تربیت بدنی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران

چکیده

مقدمه و هدف: تمرین منظم ورزشی می‌تواند یک راهکار حفاظتی و درمانی در برابر بیماری دیابت باشد. هدف از اجرای این پژوهش، بررسی اثر شش هفته تمرین هوازی فزاینده بر سطوح پروتئین های دامنه PR حاوی 16 (PRDM16) و گیرنده فعال شده تکثیر کننده پراکسیزم گاما (PPAR-y) در بافت چربی موش های دیابتی شده با استرپتوزوسین بود.
مواد و روش ها: تعداد 32 سر رت با وزن تقریبی 240-220 گرم به چهار گروه کنترل، شم، دیابت، دیابت + تمرین تقسیم شدند. گروه تمرین شش هفته و هفته‌ای پنج روز با شدت 70-60 درصد VO2max به تمرین روی نوارگردان پرداختند. برای ایجاد مدل دیابت، STZ با دوز 60 میلی­گرم در کیلوگرم در ترکیب با بافر سیترات و pH 5/4 به‌صورت درون‌صفاقی تزریق شد. سطح پروتئین های PPAR-y و PRDM16  بافت چربی سفید موش‌ها به روش الایزا اندازه­گیری شد.
نتایج: سطوح  PPAR-y و PRDM16  در گروه کنترل دیابت نسبت به کنترل کاهش معنا­دار یافت (P = 0.00). مقادیر PPAR-y و PRDM16 در گروه دیابت + تمرین در مقایسه با گروه­ کنترل دیابت افزایش معنا­دار داشت (P = 0.0001). به‌طورکلی، نتایج نشان داد که مقادیر PPAR-y و PRDM16 در گروه تمرین در مقایسه با گروه کنترل دیابت افزایش معنادار یافت.
نتیجه گیری: به‌نظر می­رسد که انجام تمرینات هوازی فزاینده به مدت هشت هفته می تواند یک روش درمانی غیر تهاجمی برای مبتلایان به اختلالات بافت چربی سفید ناشی از بیماری دیابت محسوب شود.
Background and Objective: Regular exercise can be a protective and therapeutic strategy against diabetes. The purpose of this study was to investigate the effect of six weeks of incremental aerobic training on the levels of PRDM16 and PPAR-y in adipose tissue of streptozotocin-induced diabetic rats.
Materials and Methods: 32 rats weighing 240-220 g were divided into four groups: control, sham, diabetes, diabetes + exercise. The training group practiced on the treadmill six weeks and five days a week with 60-70% VO2max intensity. To develop the model of diabetes, STZ was injected intraperitoneally at a dose of 60 mg / kg in combination with citrate buffer and pH 4.5. Levels of PPAR-y and PRDM16 white adipose tissue of rats were measured by ELISA kits.
Results: The levels of PPAR-y and PRDM16 were significantly decreased in the diabetes group (P = 0.00). PPAR-y and PRDM16 values ​​were significantly increased in the diabetic + training group (P = 0.0001). Overall, the results showed that PPAR-y and PRDM16 values ​​were significantly increased in the exercise group compared to the diabetes group.
Conclusion:Therefore, it seems that incremental aerobic exercise for eight weeks can be a non-invasive treatment for white adipose tissue disorders caused by diabetes.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of six weeks of incremental aerobic training on PRDM16 and PPAR-y protein levels in adipose tissue of streptozotocin-induced diabetic rats

نویسندگان [English]

  • Aida ghasemi Rahimbegloo
  • ABDOLLAH HASHEMVARZI
  • Abdolreza jafari chashmi
Sari Branch, Islamic Azad University, Sari, Iran
چکیده [English]

Background and Objective: Regular exercise can be a protective and therapeutic strategy against diabetes. The purpose of this study was to investigate the effect of six weeks of incremental aerobic training on the levels of PRDM16 and PPAR-y in adipose tissue of streptozotocin-induced diabetic rats.
Materials and Methods: 32 rats weighing 240-220 g were divided into four groups: control, sham, diabetes, diabetes + exercise. The training group practiced on the treadmill six weeks and five days a week with 60-70% VO2max intensity. To develop the model of diabetes, STZ was injected intraperitoneally at a dose of 60 mg / kg in combination with citrate buffer and pH 4.5. Levels of PPAR-y and PRDM16 white adipose tissue of rats were measured by ELISA kits.
Results: The levels of PPAR-y and PRDM16 were significantly decreased in the diabetes group (P = 0.00). PPAR-y and PRDM16 values were significantly increased in the diabetic + training group (P = 0.0001). Overall, the results showed that PPAR-y and PRDM16 values were significantly increased in the exercise group compared to the diabetes group.
Conclusion:Therefore, it seems that incremental aerobic exercise for six weeks can be a non-invasive treatment for white adipose tissue disorders caused by diabetes.

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دانشور پزشکی
دوره 33، شماره 1 - شماره پیاپی 176
فروردین و اردیبهشت 1404
صفحه 17-22

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