دانشور پزشکی

دانشور پزشکی

تاثیر هشت هفته تمرین مقاومتی به همراه مکمل اسپیرولینا بر تغییرات مسیر بیان ژن TNF-ɑ/ IKKβ/ TSC1/Rheb در بافت کلیه موش های سفید بزرگ نر

نوع مقاله : مقاله پژوهشی

نویسندگان
حمی رضا صادقی پور
بردیا ذاکری دهوسطی
عبدالصالح زر
محمد مهدی خالقی
گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران
10.22070/daneshmed.2025.19914.1574
چکیده
مقدمه و هدف: مسیر سیگنالی mTOR و ژن‌های وابسته به آن در اثر تمرینات مقاومتی فعال شده و موجب هایپرتروفی عضلانی می‌شود. مکمل اسپیرولینا نیز سنتز پروتئین را افزایش می‌دهد. این مطالعه به بررسی اثر هشت هفته تمرین مقاومتی و مکمل اسپیرولینا بر تغییرات مسیر بیان ژن TNF-ɑ/IKKβ/TSC1/Rheb در کلیه موش‌های نر پرداخته است..
مواد و روش ها: 32 موش صحرایی نر به‌طور تصادفی به چهار گروه کنترل، مکمل، تمرین و ترکیبی تقسیم شدند. تمرین مقاومتی طی ۸ هفته و سه ست پنج‌تکراری، یک روز در میان انجام شد. مکمل اسپیرولینا به میزان ۲۰۰ میلی‌گرم به ازای کیلوگرم وزن بدن روزانه مصرف شد. بیان ژن‌ها با روش Real-Time PCR ارزیابی و داده‌ها با آزمون تحلیل واریانس دوطرفه تحلیل شدند.
نتایج: در مقایسه با گروه کنترل، بیان ژن TNF-ɑ در گروه تمرین مقاومتی به طور معناداری بیشتر (05/0>p)، در گروه اسپیرولینا به طور معناداری کمتر (05/0>p)، و در گروه ترکیبی بدون تغییر بود (05/0<p). همچنین تمرین و ترکیب تمرین و مکمل منجر به افزایش معنادار بیان ژن IKKβ شد (05/0>p). در مقایسه با گروه کنترل، در هر سه گروه، بیان ژن TSC1 افزایش معنادار (05/0>p) و بیان ژن Rheb افزایش جزئی غیرمعناداری داشت (05/0<p).
نتیجه‌گیری: براساس نتایج تحقیق حاضر مصرف مکمل اسپیرولینا به تنهایی اثری بر مسیر سیگنالی هایپرتروفی کلیه نداشت. با این وجود تمرین مقاومتی به همراه مکمل توانست بر مسیر سیگنالی هایپرتروفی کلیوی اثرگذار باشد. تمرین مقاومتی به تنهایی توانست اثر مثبتی بر مسیر سیگنالی TNF-ɑ/IKKβ/TSC1/Rheb در هایپرتروفی کلیه بر جای بگذارد.
کلیدواژه‌ها
تمرین مقاومتی
اسپیرولینا
کلیه
فاکتور نکروز تومور‌آلفا
IKKβ
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دانشور پزشکی
دوره 32، شماره 6 - شماره پیاپی 175
بهمن و اسفند 1403
صفحه 53-67