تاثیر هشت هفته تمرین مقاومتی به همراه مکمل اسپیرولینا بر تغییرات مسیر بیان ژن TNF-ɑ/ IKKβ/ TSC1/Rheb در بافت کلیه موش های سفید بزرگ نر

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران

چکیده

مقدمه و هدف: مسیر سیگنالی mTOR و ژن‌های وابسته به آن در اثر تمرینات مقاومتی فعال شده و موجب هایپرتروفی عضلانی می‌شود. مکمل اسپیرولینا نیز سنتز پروتئین را افزایش می‌دهد. این مطالعه به بررسی اثر هشت هفته تمرین مقاومتی و مکمل اسپیرولینا بر تغییرات مسیر بیان ژن TNF-ɑ/IKKβ/TSC1/Rheb در کلیه موش‌های نر پرداخته است..
مواد و روش ها: 32 موش صحرایی نر به‌طور تصادفی به چهار گروه کنترل، مکمل، تمرین و ترکیبی تقسیم شدند. تمرین مقاومتی طی ۸ هفته و سه ست پنج‌تکراری، یک روز در میان انجام شد. مکمل اسپیرولینا به میزان ۲۰۰ میلی‌گرم به ازای کیلوگرم وزن بدن روزانه مصرف شد. بیان ژن‌ها با روش Real-Time PCR ارزیابی و داده‌ها با آزمون تحلیل واریانس دوطرفه تحلیل شدند.
نتایج: در مقایسه با گروه کنترل، بیان ژن TNF-ɑ در گروه تمرین مقاومتی به طور معناداری بیشتر (05/0>p)، در گروه اسپیرولینا به طور معناداری کمتر (05/0>p)، و در گروه ترکیبی بدون تغییر بود (05/0<p). همچنین تمرین و ترکیب تمرین و مکمل منجر به افزایش معنادار بیان ژن IKKβ شد (05/0>p). در مقایسه با گروه کنترل، در هر سه گروه، بیان ژن TSC1 افزایش معنادار (05/0>p) و بیان ژن Rheb افزایش جزئی غیرمعناداری داشت (05/0<p).
نتیجه‌گیری: براساس نتایج تحقیق حاضر مصرف مکمل اسپیرولینا به تنهایی اثری بر مسیر سیگنالی هایپرتروفی کلیه نداشت. با این وجود تمرین مقاومتی به همراه مکمل توانست بر مسیر سیگنالی هایپرتروفی کلیوی اثرگذار باشد. تمرین مقاومتی به تنهایی توانست اثر مثبتی بر مسیر سیگنالی TNF-ɑ/IKKβ/TSC1/Rheb در هایپرتروفی کلیه بر جای بگذارد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of eight weeks of resistance training combined with spirulina supplementation on the TNF-ɑ/IKKβ/TSC1/Rheb gene expression in male rat kidney tissue

نویسندگان [English]

  • Hamid Reza Sadeghipour
  • Bardia Zakeri Dehvasati
  • Abdosaleh Zar
  • Mohammad Mehdi Khaleghi
Sport Science Department, Human Faculty, Persian Gulf University, Bushehr, Iran
چکیده [English]

Background and Objective: The mTOR signaling pathway and its associated genes are activated by resistance training, leading to muscle hypertrophy. Spirulina supplementation also enhances protein synthesis. This study aimed to investigate the effects of eight weeks of resistance training and spirulina supplementation on changes in the TNF-ɑ/IKKβ/TSC1/Rheb gene expression pathway in the kidneys of male rats.
Materials and Methods: Thirty-two male rats were randomly assigned to four groups: control, supplementation, training, and combined (training + supplementation). The resistance training protocol consisted of eight weeks of exercises performed every other day, with three sets of five repetitions per session. Spirulina supplementation was administered daily at a dose of 200 mg per kg of body weight. Gene expression levels were evaluated using the Real-Time PCR method, and data were analyzed using two-way ANOVA.
Results: Compared to the control group, TNF-ɑ gene expression significantly increased in the resistance training group (p < 0.05), decreased in the spirulina group (p < 0.05), and remained unchanged in the combination group (p > 0.05). Resistance training and the combined intervention significantly increased IKKβ gene expression (p < 0.05). Compared to the control group, all three experimental groups showed a significant increase in TSC1 gene expression (p < 0.05), while the increase in Rheb gene expression was slight and not statistically significant (p > 0.05).
Conclusion: The findings of this study indicate that spirulina supplementation alone had no significant effect on the hypertrophic signaling pathway in the kidney. However, resistance training combined with spirulina supplementation influenced the renal hypertrophic signaling pathway. Resistance training alone positively impacted the TNF-ɑ/IKKβ/TSC1/Rheb pathway in kidney hypertrophy.

کلیدواژه‌ها [English]

  • Resistance training
  • Spirulina
  • Kidney
  • Tumor Necrosis Factor-Alpha
  • IKKβ

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دانشور پزشکی
دوره 32، شماره 6 - شماره پیاپی 175
بهمن و اسفند 1403
صفحه 53-67

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