دانشور پزشکی

دانشور پزشکی

تاثیر تمرین تناوبی با شدت بالا (HIIT) بر محتوای پروتئین‌های eIF2α، ATF4 و CHOP در بطن چپ قلب موش‌های سفید بزرگ آزمایشگاهی چاق مبتلا به دیابت

نوع مقاله : مقاله پژوهشی

نویسندگان
حامد علیزاده پهلوانی 1
محمد شرافتی مقدم 2
فاطمه صلح دوست 2
1 گروه آموزش تربیت بدنی، دانشگاه فرهنگیان، تهران، ایران
2 گروه علوم ورزشی، مؤسسه‌ی آموزش عالی آپادانا، شیراز، ایران
10.22070/daneshmed.2025.19909.1573
چکیده
مقدمه و هدف: شیوع چاقی و دیابت یک اپیدمی است و خطراتی را برای بهداشت عمومی ایجاد می‌کند. از این رو هدف از مطالعه حاضر تاثیر تمرین تناوبی با شدت بالا (HIIT) بر پروتئین‌های eIF2α، ATF4 و CHOP در بطن چپ قلب موش‌های سفید بزرگ آزمایشگاهی دیابتی چاق بود.
مواد و روش ها: تحقیق حاضر تجربی-بنیادی بود. 16 موش‌ سفید بزرگ آزمایشگاهی 2 ماهه نر اسپراگوداولی با وزن 20±180 گرم خریداری شدند. برای القای دیابت نوع 2، محلول نیکوتین‌آمید و استرپتوزوتوسین با دوز 110 و 60 mg/kg تزریق شد. قند خون بین 126 تا 260 mg/dl شاخص دیابت نوع 2 در نظر گرفته شد. موش‌های سفید بزرگ آزمایشگاهی به گروه کنترل و  HIIT تقسیم شدند. گروه HIIT به مدت 8 هفته و 3 روز در هفته و در هر جلسه 5 نوبت 4 دقیقه‌ای با شدت معادل 85-95% حداکثر سرعت همراه با دوره‌های استراحت فعال 3 دقیقه‌ای با شدت معادل 50-%60 درصد حداکثر سرعت تمرین کردند. بعد از 48  ساعت از آخرین جلسه تمرین، موش‌های سفید بزرگ آزمایشگاهی بی‌هوش شدند و بافت بطن چپ برداشته شد و با روش وسترن بلات پروتئین‌های مورد نظر سنجش شدند. آزمون شاپیرو-ویلک و t-مستقل برای بررسی داده‌ها استفاده شد و سطح معناداری 05/0≥P در نظر گرفته شد.
نتایج: پس از 8 هفته HIIT، محتوای پروتئین eIF2α (001/0P=)، ATF4 (001/0P=) و پروتئین CHOP (001/0P=) افزایش معنی‌داری را نسبت به گروه کنترل در بطن چپ قلب نشان داد.
نتیجه‌گیری: اثرات HIIT بر روی پروتئین‌های eIF2B، ATF4 و CHOP احتمالاً سازگاری‌های سلولی را القا می‌کند که می‌تواند از طریق تعامل این پروتئین‌ها یک روش موثر برای موش‌های سفید بزرگ آزمایشگاهی دیابتی چاق باشد.
کلیدواژه‌ها
دیابت
برنامه HIIT
چاقی
بطن چپ
eIF2α
ATF4
موضوعات
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دانشور پزشکی
دوره 32، شماره 6 - شماره پیاپی 175
بهمن و اسفند 1403
صفحه 41-52