تاثیر تمرین تناوبی با شدت بالا (HIIT) بر محتوای پروتئین‌های eIF2α، ATF4 و CHOP در بطن چپ قلب موش‌های سفید بزرگ آزمایشگاهی چاق مبتلا به دیابت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه آموزش تربیت بدنی، دانشگاه فرهنگیان، تهران، ایران

2 گروه علوم ورزشی، مؤسسه‌ی آموزش عالی آپادانا، شیراز، ایران

چکیده

مقدمه و هدف: شیوع چاقی و دیابت یک اپیدمی است و خطراتی را برای بهداشت عمومی ایجاد می‌کند. از این رو هدف از مطالعه حاضر تاثیر تمرین تناوبی با شدت بالا (HIIT) بر پروتئین‌های eIF2α، ATF4 و CHOP در بطن چپ قلب موش‌های سفید بزرگ آزمایشگاهی دیابتی چاق بود.
مواد و روش ها: تحقیق حاضر تجربی-بنیادی بود. 16 موش‌ سفید بزرگ آزمایشگاهی 2 ماهه نر اسپراگوداولی با وزن 20±180 گرم خریداری شدند. برای القای دیابت نوع 2، محلول نیکوتین‌آمید و استرپتوزوتوسین با دوز 110 و 60 mg/kg تزریق شد. قند خون بین 126 تا 260 mg/dl شاخص دیابت نوع 2 در نظر گرفته شد. موش‌های سفید بزرگ آزمایشگاهی به گروه کنترل و  HIIT تقسیم شدند. گروه HIIT به مدت 8 هفته و 3 روز در هفته و در هر جلسه 5 نوبت 4 دقیقه‌ای با شدت معادل 85-95% حداکثر سرعت همراه با دوره‌های استراحت فعال 3 دقیقه‌ای با شدت معادل 50-%60 درصد حداکثر سرعت تمرین کردند. بعد از 48  ساعت از آخرین جلسه تمرین، موش‌های سفید بزرگ آزمایشگاهی بی‌هوش شدند و بافت بطن چپ برداشته شد و با روش وسترن بلات پروتئین‌های مورد نظر سنجش شدند. آزمون شاپیرو-ویلک و t-مستقل برای بررسی داده‌ها استفاده شد و سطح معناداری 05/0≥P در نظر گرفته شد.
نتایج: پس از 8 هفته HIIT، محتوای پروتئین eIF2α (001/0P=)، ATF4 (001/0P=) و پروتئین CHOP (001/0P=) افزایش معنی‌داری را نسبت به گروه کنترل در بطن چپ قلب نشان داد.
نتیجه‌گیری: اثرات HIIT بر روی پروتئین‌های eIF2B، ATF4 و CHOP احتمالاً سازگاری‌های سلولی را القا می‌کند که می‌تواند از طریق تعامل این پروتئین‌ها یک روش موثر برای موش‌های سفید بزرگ آزمایشگاهی دیابتی چاق باشد.

کلیدواژه‌ها

موضوعات

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دانشور پزشکی
دوره 32، شماره 6 - شماره پیاپی 175
بهمن و اسفند 1403
صفحه 41-52

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