تاثیر تمرین تناوبی با شدت بالا (HIIT) بر محتوای پروتئین‌های eIF2α، ATF4 و CHOP در بطن چپ قلب موش‌های سفید بزرگ آزمایشگاهی چاق مبتلا به دیابت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه آموزش تربیت بدنی، دانشگاه فرهنگیان، تهران، ایران

2 گروه علوم ورزشی، مؤسسه‌ی آموزش عالی آپادانا، شیراز، ایران

چکیده

مقدمه و هدف: شیوع چاقی و دیابت یک اپیدمی است و خطراتی را برای بهداشت عمومی ایجاد می‌کند. از این رو هدف از مطالعه حاضر تاثیر تمرین تناوبی با شدت بالا (HIIT) بر پروتئین‌های eIF2α، ATF4 و CHOP در بطن چپ قلب موش‌های سفید بزرگ آزمایشگاهی دیابتی چاق بود.
مواد و روش ها: تحقیق حاضر تجربی-بنیادی بود. 16 موش‌ سفید بزرگ آزمایشگاهی 2 ماهه نر اسپراگوداولی با وزن 20±180 گرم خریداری شدند. برای القای دیابت نوع 2، محلول نیکوتین‌آمید و استرپتوزوتوسین با دوز 110 و 60 mg/kg تزریق شد. قند خون بین 126 تا 260 mg/dl شاخص دیابت نوع 2 در نظر گرفته شد. موش‌های سفید بزرگ آزمایشگاهی به گروه کنترل و  HIIT تقسیم شدند. گروه HIIT به مدت 8 هفته و 3 روز در هفته و در هر جلسه 5 نوبت 4 دقیقه‌ای با شدت معادل 85-95% حداکثر سرعت همراه با دوره‌های استراحت فعال 3 دقیقه‌ای با شدت معادل 50-%60 درصد حداکثر سرعت تمرین کردند. بعد از 48  ساعت از آخرین جلسه تمرین، موش‌های سفید بزرگ آزمایشگاهی بی‌هوش شدند و بافت بطن چپ برداشته شد و با روش وسترن بلات پروتئین‌های مورد نظر سنجش شدند. آزمون شاپیرو-ویلک و t-مستقل برای بررسی داده‌ها استفاده شد و سطح معناداری 05/0≥P در نظر گرفته شد.
نتایج: پس از 8 هفته HIIT، محتوای پروتئین eIF2α (001/0P=)، ATF4 (001/0P=) و پروتئین CHOP (001/0P=) افزایش معنی‌داری را نسبت به گروه کنترل در بطن چپ قلب نشان داد.
نتیجه‌گیری: اثرات HIIT بر روی پروتئین‌های eIF2B، ATF4 و CHOP احتمالاً سازگاری‌های سلولی را القا می‌کند که می‌تواند از طریق تعامل این پروتئین‌ها یک روش موثر برای موش‌های سفید بزرگ آزمایشگاهی دیابتی چاق باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of high-intensity interval training (HIIT) on the content of eIF2α, ATF4 and CHOP proteins in the left ventricle of obese diabetic rats

نویسندگان [English]

  • Hamed Alizadeh Pahlavani 1
  • Mohammad Sherafati Moghadam 2
  • Fatemeh Solhdoust 2
1 Department of Physical Education, Farhangian University, Tehran, Iran
2 Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran
چکیده [English]

Background and Objective: The prevalence of obesity and diabetes is an epidemic and poses risks to public health. Therefore, the aim of the present study was to determine the effect of HIIT on the content of eIF2α, ATF4 and CHOP proteins in the left ventricle of obese diabetic rats.
Materials and Methods: The present study was experimental-basic. Twelve 2-month-old male Sprague-Dawley rats weighing 180±20 g were purchased. To induce type 2 diabetes, nicotinamide and streptozotocin solutions were injected at doses of 110 and 60 mg/kg, respectively. Blood sugar between 126 and 260 mg/dl was considered an index of type 2 diabetes. Rats were divided into control and HIIT groups. The HIIT group trained for 8 weeks, 3 days a week, with 5 4-minute sessions at an intensity of 85-95% of maximum speed, followed by 3-minute active rest periods at an intensity of 50-60% of maximum speed. After 48 hours from the last training session, the rats were anesthetized and left ventricular tissue was removed and the desired proteins were measured by Western blotting. The Shapiro-Wilk test and independent t-test were used to examine the data, and a significance level of P < 0.05 was considered.
Results: After 8 weeks of HIIT, the content of eIF2α (P=0.001), ATF4 (P=0.001), and CHOP (P=0.001) proteins in the left ventricle of the heart showed a significant increase compared to the control group.
Conclusion: The effects of HIIT on eIF2B, ATF4, and CHOP proteins likely induce cellular adaptations that could be an effective treatment for obese diabetic rats through the interaction of these proteins

کلیدواژه‌ها [English]

  • Diabetes
  • HIIT program
  • Obesity
  • Left ventricle
  • eIF2α
  • ATF4
  • CHOP

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دانشور پزشکی
دوره 32، شماره 6 - شماره پیاپی 175
بهمن و اسفند 1403
صفحه 41-52

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