مقایسه تمرین تداومی با و بدون محدودیت کالریک بر بیان ژن لپتین و آدیپونکتین در بافت چربی و مقاومت به انسولین در موش های بزرگ سالمند

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

مقدمه و هدف: بافت چربی با آزادسازی آدیپوکاین‌ها نقش مهمی در متابولیسم و التهاب بدن دارد و محدودیت کالری و تمرین تداومی با تأثیر بر بافت چربی می‌تواند بر التهاب و روند پیری تأثیر بگذارد، لذا هدف این تحقیق بررسی تأثیر محدودیت کالریک و تمرین تداومی بر بیان ژن لپتین، آدیپونکتین، نسبت آدیپونکتین به لپتین و مقاومت به انسولین در موش‌های سفید بزرگ آزمایشگاهی‌های سالمند بود.
مواد و روش ها: 36 موش‌های سفید بزرگ آزمایشگاهی سالمند (26 ماه) ویستار نر به طور تصادفی به چهار گروه تقسیم شدند: کنترل، محدودیت کالریک، تمرین تداومی و محدودیت کالریک+ تمرین تداومی. پروتکل تمرین تداومی و محدودیت کالری به مدت شش هفته و هر هفته 5 جلسه انجام شد. سپس از بافت چربی اپیدیدیم موش‌های سفید بزرگ آزمایشگاهی‌ نمونه‌برداری انجام شد و از روش Real Time PCR برای اندازه‌گیری بیان ژن‌های آدیپونکتین و لپتین استفاده شد. برای مقاومت به انسولین، شاخص HOMA-IR گزارش شد. تجزیه و تحلیل آماری با استفاده از آزمون آنالیز کروسکال-والیس و یومن ویتنی و در سطح معنادار 05/0 صورت پذیرفت.
نتایج: نتایج نشان داد که بیان ژن آدیپونکتین در گروه‌های محدودیت کالری و تمرین تداومی افزایش معنی‌داری یافته است اما در گروه محدودیت کالری به همراه تمرین تداومی بیان ژن آدیپونکتین کاهش یافته است. از سوی دیگر، نتایج نشان داد که تفاوت معنی‌داری بین گروه‎‌ها در بیان ژن لپتین، نسبت بیان ژن آدیپونکتین به لپتین و مقاومت به انسولین در بافت چربی وجود ندارد.
نتیجه‌گیری: نشان دادند که در حالی که محدودیت کالری و تمرین تداومی اثرات مفیدی بر افزایش بیان ژن آدیپونکتین دارند اما ترکیب آنها ممکن است همیشه به هم افزایی منجر نشود.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of continuous training with and without caloric restriction on leptin and adiponectin gene expression in adipose tissue and insulin resistance in elderly rats

نویسندگان [English]

  • Saeed Rezaee
  • Mahtab Moazzami
  • Nahid Bijeh
  • Alireza Hosseini Kakhk
  • Mohammad Mosaferi Ziaaldini
Department of Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Iran
چکیده [English]

Background and Objective: Adipose tissue plays a crucial role in body metabolism and inflammation by releasing adipokines. Both caloric restriction and continuous exercise can impact adipose tissue and affect inflammation and the aging process. Therefore, the aim of this research was to investigate the effect of caloric restriction and continuous exercise on the expression of the leptin gene, adiponectin, the ratio of adiponectin to leptin, and insulin resistance in aged rats.
Materials and Methods: The study included a total of 36 aged male Wistar rats (26 months old), randomly divided into four groups: control, restriction, caloric, and continuous exercise, as well as caloric restriction+continuous exercise. The rats underwent a six-week continuous training protocol, with five sessions per week. Samples were then taken from the epididymal fat tissue of the rats, and Real-Time PCR was used to measure the expression of the adiponectin and leptin genes. Statistical analysis was conducted using non-parametric statistics.
Results: The statistical tests showed that the expression of the adiponectin gene significantly increased in the caloric restriction and continuous exercise groups. However, in the caloric restriction+continuous exercise group, the expression of the adiponectin gene decreased. Furthermore, there was no significant difference in leptin gene expression, the ratio of adiponectin to leptin gene expression, or insulin resistance in fat tissue among the groups.
Conclusion: These findings suggest that while caloric restriction and continuous exercise have beneficial effects on increasing adiponectin gene expression, their combination may not always result in synergy.

کلیدواژه‌ها [English]

  • Continuous training
  • Calorie restriction
  • Aging
  • Adiponectin
  • Leptin
  • Insulin resistance

مراجع

  1. Fougère B, Boulanger E, Nourhashemi F, Guyonnet S, Cesari M. Chronic Inflammation: Accelerator of Biological Aging. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences. 2017;72:1218–25.
  2. Chait A, den Hartigh LJ. Adipose Tissue Distribution, Inflammation and Its Metabolic Consequences, Including Diabetes and Cardiovascular Disease. Frontiers in Cardiovascular Medicine. 2020;7.
  3. Mancuso P, Bouchard B. The Impact of Aging on Adipose Function and Adipokine Synthesis. Frontiers in Endocrinology. 2019;10.
  4. Gulcelik NE, Halil M, Ariogul S, Usman A. Adipocytokines and aging: adiponectin and leptin. Minerva Endocrinology. 2013;38(2):203-10.
  5. Vatier C, Antuna-Puente B, Fellahi S, Vigouroux C, Capeau J, Bastard JP. The adiponectin to leptin ratio, a still unrecognized biomarker of insulin resistance and cardiometabolic risk. Annales de Biologie Clinique. 2020;78(3):265-8.
  6. Paz-Filho G, Mastronardi C, Franco CB, Wang KB, Wong ML, Licinio J. Leptin: molecular mechanisms, systemic pro-inflammatory effects, and clinical implications. Arquivos Brasileiros de Endocrinologia e Metabologia. 2012;56(9):597-607.
  7. Francisco V, Pino J, Campos-Cabaleiro V, Ruiz-Fernández C, Mera A, Gonzalez-Gay MA, et al. Obesity, Fat Mass and Immune System: Role for Leptin. Frontiers in Physiology. 2018;9:640.
  8. Fang H, Judd RL. Adiponectin Regulation and Function. Comprehensive Physiology. 2018;8(3):1031-63.
  9. Yoon MJ, Lee GY, Chung JJ, Ahn YH, Hong SH, Kim JB. Adiponectin increases fatty acid oxidation in skeletal muscle cells by sequential activation of AMP-activated protein kinase, p38 mitogen-activated protein kinase, and peroxisome proliferator-activated receptor alpha. Diabetes. 2006;55(9):2562-70.
  10. Wang ZV, Scherer PE. Adiponectin, the past two decades. Journal of Molecular Cell Biology. 2016;8 2:93-100.
  11. Frühbeck G, Catalán V, Rodríguez A, Ramírez B, Becerril S, Salvador J, et al. Adiponectin-leptin Ratio is a Functional Biomarker of Adipose Tissue Inflammation. Nutrients. 2019;11(2).
  12. Liu Y, Hong F, Lebaka VR, Mohammed A, Ji L, Zhang Y, Korivi M. Calorie Restriction With Exercise Intervention Improves Inflammatory Response in Overweight and Obese Adults: A Systematic Review and Meta-Analysis. Frontiers in Physiology. 2021;12:754731.
  13. Kökten T, Hansmannel F, Ndiaye NC, Heba AC, Quilliot D, Dreumont N, et al. Calorie Restriction as a New Treatment of Inflammatory Diseases. Advances in Nutrition. 2021;12(4):1558-70.
  14. Figueiredo L, Barcos Nunes R, Marmett B, Sá L, Arbex A. Anti-Inflammatory Effects of Physical Exercise on Obesity. Open Journal of Endocrine and Metabolic Diseases. 2017;07:44-51.
  15. Su M, Bai Y, Song W, Wang M, Shen R-R, Du K-J, et al. Effect of exercise on adiponectin in aged obese rats. Zhongguo ying yong sheng li xue za zhi. Chinese Journal of Applied Physiology. 2018;34 4:345-9.
  16. La Russa D, Marrone A, Mandalà M, Macirella R, Pellegrino D. Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese Rat Model: The Role of Adiponectin. Biomedicines. 2020;8(12).
  17. Senkus KE, Crowe-White KM, Bolland AC, Locher JL, Ard JD. Changes in adiponectin:leptin ratio among older adults with obesity following a 12-month exercise and diet intervention. Nutrition & Diabetes. 2022;12.
  18. Oki K, Arias EB, Kanzaki M, Cartee GD. Effects of Acute Exercise Combined with Calorie Restriction Initiated Late-in-Life on Insulin Signaling, Lipids and Glucose Uptake in Skeletal Muscle from Old Rats. The Journals of Gerontology Series A, Biological Sciences and Medical Sciences. 2018.
  19. Wang H, Sharma N, Arias EB, Cartee GD. Insulin Signaling and Glucose Uptake in the Soleus Muscle of 30-Month-Old Rats After Calorie Restriction With or Without Acute Exercise. The Journals of Gerontology Series A, Biological Sciences and Medical Sciences. 2016;71 3:323-32.
  20. Ding Q, Ash C, Mráček T, Merry BJ, Bing C. Caloric restriction increases adiponectin expression by adipose tissue and prevents the inhibitory effect of insulin on circulating adiponectin in rats. The Journal of Nutritional Biochemistry. 2012;23 8:867-74.
  21. Zhu M, Miura J, Lu LX, Bernier M, Decabo R, Lane MA, et al. Circulating adiponectin levels increase in rats on caloric restriction: the potential for insulin sensitization. Experimental Gerontology. 2004;39:1049-59.
  22. Ding Q, Ash C, Mracek T, Merry B, Bing C. Caloric restriction increases adiponectin expression by adipose tissue and prevents the inhibitory effect of insulin on circulating adiponectin in rats. The Journal of Nutritional Biochemistry. 2012;23(8):867-74.
  23. Boudou P, Sobngwi E, Mauvais-Jarvis F, Vexiau P, Gautier J. Absence of exercise-induced variations in adiponectin levels despite decreased abdominal adiposity and improved insulin sensitivity in type 2 diabetic men. European Journal of Endocrinology. 2003;149(5):421-4.
  24. Hulver MW, Zheng D, Tanner CJ, Houmard JA, Kraus WE, Slentz CA, et al. Adiponectin is not altered with exercise training despite enhanced insulin action. American Journal of Physiology-Endocrinology And Metabolism. 2002;283(4):E861-E5.
  25. Lee S, Kwak HB. Effects of interventions on adiponectin and adiponectin receptors. Journal of Exercise Rehabilitation. 2014;10(2):60-8.
  26. Kraemer RR, Aboudehen KS, Carruth AK, Durand RT, Acevedo EO, Hebert EP, et al. Adiponectin responses to continuous and progressively intense intermittent exercise. Medecine Science Sports Exercise. 2003;35(8):1320-5.
  27. Lee S, Kwak H-B. Effects of interventions on adiponectin and adiponectin receptors. Journal of Exercise Rehabilitation. 2014;10:60 - 8.
  28. Qiao L, Lee B, Kinney B, Yoo Hs, Shao J. Energy intake and adiponectin gene expression. American Journal of Physiology Endocrinology and Metabolism. 2011;300 5:E809-16.
  29. Kubota N, Yano W, Kubota T, Yamauchi T, Itoh S, Kumagai H, et al. Adiponectin stimulates AMP-activated protein kinase in the hypothalamus and increases food intake. Cell Metabolism. 2007;6 1:55-68.
  30. Fasshauer M, Kralisch S, Klier M, Lossner U, Bluher M, Klein JC, Paschke R. Adiponectin gene expression and secretion is inhibited by interleukin-6 in 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications. 2003;301 4:1045-50.
  31. Bauche IB, Ait El Mkadem S, Rezsohazy R, Funahashi T, Maeda N, Miranda LM, Brichard SM. Adiponectin downregulates its own production and the expression of its AdipoR2 receptor in transgenic mice. Biochemical and Biophysical Research Communications. 2006;345 4:1414-24.
  32. Ghadge AA, Khaire AA, Kuvalekar AA. Adiponectin: A potential therapeutic target for metabolic syndrome. Cytokine & Growth Factor Reviews. 2018;39:151-8.
  33. Christiansen T, Paulsen SK, Bruun JM, Ploug T, Pedersen SB, Richelsen B. Diet-induced weight loss and exercise alone and in combination enhance the expression of adiponectin receptors in adipose tissue and skeletal muscle, but only diet-induced weight loss enhanced circulating adiponectin. The Journal of Clinical Endocrinology and Metabolism. 2010;95 2:911-9.
  34. Bouassida A, Chamari K, Zaouali M, Feki Y, Zbidi A, Tabka Z. Review on leptin and adiponectin responses and adaptations to acute and chronic exercise. British Journal of Sports Medicine. 2010;44(9):620-30.
  35. Carter S, Caron A, Richard D, Picard F. Role of leptin resistance in the development of obesity in older patients. Clinical Interventions in Aging. 2013:829-44.
  36. Shahidi F, pirhadi s. The Effect of Physical exercise and training on serum leptin level. Razi Journal of Medical Sciences. 2014;21:1-14.
  37. Bouassida A, Zalleg D, Bouassida S, Zaouali M, Feki Y, Zbidi A, Tabka Z. Leptin, its implication in physical exercise and training: a short review. Journal of Sports Science & Medicine. 2006;5 2:172-81.
  38. Hulver MW, Houmard JA. Plasma Leptin and Exercise. Sports Medicine. 2003;33:473-82.
  39. Flores MBS, Fernandes MF, Ropelle ER, Faria MC, Ueno M, Velloso LA, et al. Exercise Improves Insulin and Leptin Sensitivity in Hypothalamus of Wistar Rats. Diabetes. 2006;55:2554 - 61.
  40. WANG Z-W, PAN W-T, Lee Y, Kakuma T, ZHOU Y-T, Unger RH. The role of leptin resistance in the lipid abnormalities of aging. The FASEB Journal. 2001;15(1):108-14.
  41. Mizuno T, Bergen H, Kleopoulos S, Bauman W, Mobbs C. Effects of nutritional status and aging on leptin gene expression in mice: importance of glucose. Hormone and Metabolic Research. 1996;28(12):679-84.
  42. López-Jaramillo P, Gómez-Arbeláez D, López-López J, López-López C, Martínez-Ortega J, Gómez-Rodríguez A, Triana-Cubillos S. The role of leptin/adiponectin ratio in metabolic syndrome and diabetes. Hormone Molecular Biology and Clinical Investigation. 2014;18(1):37-45 .
  43. Kelly KR, Navaneethan SD, Solomon TP, Haus JM, Cook M, Barkoukis H, Kirwan JP. Lifestyle-induced decrease in fat mass improves adiponectin secretion in obese adults. Medecine Science Sports Exercise. 2014;46(5):920-6.
  44. Abbenhardt C, McTiernan A, Alfano CM, Wener MH, Campbell KL, Duggan C, et al. Effects of individual and combined dietary weight loss and exercise interventions in postmenopausal women on adiponectin and leptin levels. Journal International Medecine. 2013;274(2):163-75.
  45. McAuley KA, Williams SM, Mann JI, Goulding A, Chisholm AW, Wilson NC, et al. Intensive lifestyle changes are necessary to improve insulin sensitivity: a randomized controlled trial. Diabetes Care. 2002;25 3:445-52.
  46. Escrivá F, Gavete ML, Fermín Y, Pérez C, Gallardo N, Alvarez C, et al. Effect of age and moderate food restriction on insulin sensitivity in Wistar rats: role of adiposity. Journal Endocrinology. 2007;194(1):131-41.
  47. Craig BW, Garthwaite SM, Holloszy JO. Adipocyte insulin resistance: effects of aging, obesity, exercise, and food restriction. Journal of Applied Physiology. 1987;62 1:95-100.
  48. Kodama S, Shu M, Saito K, Murakami H, Tanaka K, Kuno S, et al. Even low-intensity and low-volume exercise training may improve insulin resistance in the elderly. Internal Medicine. 2007;46 14:1071-7.
  49. Ryan AS. Insulin Resistance with Aging. Sports Medicine. 2000;30:327-46.
  50. Scheen A. Diabetes mellitus in the elderly: insulin resistance and/or impaired insulin secretion? Diabetes & Metabolism. 2005;31 Spec No 2:5S27-5S34.

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