تاثیر تمرین مقاومتی بر محتوای پروتئین‌های مرتبط با استرس شبکه آندوپلاسمی (PERK و NRF2) در بطن چپ موش‌های سفید بزرگ آزمایشگاهی مبتلا به دیابت نوع 2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم ورزشی، مؤسسه‌ی آموزش عالی آپادانا، شیراز، ایران

2 گروه آموزش تربیت بدنی، دانشگاه فرهنگیان، تهران، ایران

چکیده

مقدمه و هدف: در سال‌های اخیر تمرین مقاومتی به عنوان یک مداخله قوی برای بهبود سلامت عملکرد قلبی عروقی در افراد مبتلا به دیابت نوع 2 پیشنهاد شده است. از این رو، هدف از مطالعه حاضر تاثیر تمرین مقاومتی بر محتوای پروتئین‌های مرتبط با استرس شبکه آندوپلاسمی (PERK و NRF2) در بطن چپ موش‌های سفید بزرگ آزمایشگاهی مبتلا به دیابت نوع 2 است. 
 مواد و روش ها: در این مطالعه‌ی تجربی، 12 سر موش‌ سفید بزرگ ازمایشگاهی نر 2 ماهه از نژاد Sprague Dawley با میانگین وزن 20±280 گرم انتخاب شدند. دیابتی نوع 2 از طریق تزریق محلول‌های نیکوتین‌آمید و استرپتوزوتوسین القاء شد. موش‌های سفید بزرگ آزمایشگاهی به روش تصادفی به 2 گروه، تمرین مقاومتی دیابتی و کنترل دیابتی تقسیم شدند؛ تمرین مقاومتی شامل 8 هفته و هفته‌ای 3 جلسه بالارفتن از یک نردبان عمودی با شیب 85 درجه به طول یک متر با 26 پله و 2 سانتی‌متر فضای بین هر پله بود. برای سنجش محتوای پروتئین PERK و NRF2 از روش وسترن‌بلات استفاده شد. برای تجزیه‌ و تحلیل داده‌ها از آزمون‌ t-مستقل استفاده‏ شد و سطح معناداری 05/0P≤ بود.
نتایج: پس از 8 هفته تمرین مقاومتی محتوای پروتئین‌های PERK و NRF2 افزایش معنی‌داری را نسبت به گروه کنترل در بطن چپ قلب موش‌های سفید بزرگ آزمایشگاهی دیابتی نشان داد (به تریتب 01/0P= و 002/0P=).
نتیجه‌گیری: به نظر می‌رسد تمرین مقاومتی برای سلامت قلب آزمودنی‌های مبتلا به دیابت نوع 2 می‌تواند مفید باشد و در دستورالعمل توانبخشی متخصصان سلامتی قرار گیرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of resistance training on the content of proteins associated with endoplasmic reticulum stress (PERK and NRF2) in the left ventricle of rats with type 2 diabetes

نویسندگان [English]

  • Fatemeh Solhdoust 1
  • Hamed Alizadeh Pahlavani 2
  • Mohammad Sherafati Moghadam 1
1 Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran
2 Department of Physical Education, Farhangian University, Tehran, Iran
چکیده [English]

Background and Objective: In recent years, resistance training has been proposed as a powerful intervention to improve the health of cardiovascular function in people with type 2 diabetes. Therefore, the aim of this study is the effect of resistance training on the content of endoplasmic reticulum stress-related proteins (PERK and NRF2) in the left ventricle of rats with type 2 diabetes.
Materials and Methods: In this experimental study, 12 2-month-old male Sprague Dawley rats with an average weight of 280±20 grams were selected. Type 2 diabetes was induced by injecting nicotinamide and streptozotocin solutions. Rats were randomly divided into 2 groups, diabetic resistance training and diabetic control. Resistance training consisted of 8 weeks and 3 sessions per week of climbing a vertical ladder with an 85 degree slope, one meter long with 26 steps and 2 cm space between each step. Western blot method was used to measure PERK and NRF2 protein content. Independent t-test was used for data analysis and the significance level was P≤0.05.
Results: After 8 weeks of resistance training, the content of PERK and NRF2 proteins showed a significant increase compared to the control group in the left ventricle of the heart of diabetic rats (P=0.01 and P=0.002, respectively).
Conclusion: It seems that resistance training can be useful for the heart health of subjects with type 2 diabetes and can be included in the rehabilitation guidelines of health professionals.

کلیدواژه‌ها [English]

  • Resistance exercise
  • Endoplasmic reticulum
  • Left ventricle
  • PERK protein
  • NRF2 protein
  • Type 2 diabetes

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دانشور پزشکی
دوره 32، شماره 4 - شماره پیاپی 173
مهر و آبان 1403
صفحه 36-46

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