تأثیر یک دوره تمرین تناوبی با شدت بالا همراه با رژیم غذایی پرچرب بر بیان ماکروفاژهای M1و M2 در عضله گاستروکنمیوس موش های بزرگ نر نژاد ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی و تندرستی، دانشگاه تهران، تهران، ایران

2 گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

10.22070/daneshmed.2024.19291.1510

چکیده

مقدمه و هدف: فعالیت ورزشی در جنبه‌های مختلفی مانند سیستم عضلانی بررسی می‌شود. این پژوهش به بررسی تأثیر یک دوره تمرین تناوبی شدت بالا همراه با رژیم غذایی پرچرب بر بیان ماکروفاژهای M1 و M2 در عضله گاستروکنمیوس رت‌های نر نژاد ویستار می‌پردازد.
مواد و روش ها: تعداد ٢٤ سررت نر بالغ سالم نژاد ویستار با میانگین وزن 15±195 گرم و سن 6 هفته به‌طور تصادفی به ٤ گروه کنترل، تمرین تناوبی با شدت بالا، تغذیه پرچرب و تمرین تناوب با شدت بالا با تغذیه پرچرب تقسیم شدند. مداخلات، شامل 10 هفته تمرینات تناوبی با شدت بالا و تغذیه پرچرب بود. سپس، نمونه‌برداری از عضله گاستروکنمیوس انجام شد. مقادیر ماکروفاژهای M1 و M2 با استفاده از روش Real-Time-PCR اندازه‌گیری شد. تجزیه تحلیل داده­ با استفاده از تحلیل واریانس یک­طرفه در سطح معنی­داری) 05/0P<) انجام شد.
اختلاف معنی‌داری بین گروه‌ها در بیان ماکروفاژهای M1  (0004/0P=) و M2 (0023/0P=) مشاهده شد. بیان ماکروفاژ M1 در گروه تغذیه پرچرب نسبت به گروه تمرین تناوبی با شدت بالا به‌طور معنی‌داری بالاتر (0001/0=P) بود. بیان ماکروفاژ M1 در گروه تمرین تناوبی با شدت بالا با تغذیه پرچرب نسبت به گروه تغذیه پرچرب به طورمعنی­داری کمتربود (0001/0P=)، اما بیان ماکروفاژ M2 درگروه تمرین تناوبی با شدت بالا با تغذیه پرچرب نسبت به گروه تغذیه پرچرب به طورمعنی­داری بالاتر (0003/0=P) بود.
نتیجه‌گیری: با توجه به نتایج به نظر می‌رسد تمرینات تناوبی با شدت بالا می‌تواند بیان ماکروفاژهای M1 را کاهش و ماکروفاژهای M2 را افزایش دهد و از اثرات منفی تغذیه پرچرب جلوگیری کند.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of high-intensity interval training with a high-fat diet on the expression of m1 and m2 macrophages in the gastrocnemius muscle of male wistar rats

نویسندگان [English]

  • Alireza Fatahian 1
  • Mohammadreza Kordi 1
  • Ali Asghar Ravasi 1
  • reza Gharakhanlou 2
1 Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran
2 Department of Sports Physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Background and Objective: Physical activity is examined in various aspects, such as the structure of the muscular system. This study investigated the impact of a high-intensity interval training (HIIT) program combined with a high-fat diet on the expression of M1 and M2 macrophages in the gastrocnemius muscle of male Wistar rats.
Materials and Methods: 24 healthy adult male Wistar rats with an average weight of 195±15 grams and age of 6 weeks were randomly divided into 4 groups: control, HIIT, high-fat diet, and HIIT with a high-fat diet. Interventions included 10 weeks of HIIT and a high-fat diet. Subsequently, samples were collected from the gastrocnemius muscle. The levels of M1 and M2 macrophages were measured using Real-Time-PCR. Data analysis was performed using one-way analysis of variance at a significance level of P<0.05.
Results: Significant differences were observed between groups in the expression of M1 (P=0.0004) and M2 (P=0.0023) macrophages. M1 macrophage expression was significantly higher in the high-fat diet group compared to the HIIT group (P=0.0001). M1 macrophage expression was significantly lower in the HIIT with a high-fat diet group compared to the high-fat diet group (P=0.0001), but M2 macrophage expression was significantly higher in the HIIT with a high-fat diet group compared to the high-fat diet group (P=0.0003).
Conclusion: These findings suggest that HIIT can reduce M1 macrophage expression and increase M2 macrophage expression, mitigating the negative effects of a high-fat diet.

کلیدواژه‌ها [English]

  • High-intensity interval training
  • High-fat diet
  • Macrophage m1
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