تاثیر تمرین هوازی تداومی همراه با مصرف بهار نارنج بر بیان ژن‌های HM و MOTS-C میتوکندری بافت کبد موش های بزرگ سالمند

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی وعلوم ورزشی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران

10.22070/daneshmed.2024.19122.1493

چکیده

مقدمه و هدف: روند افزایش سن با اختلال در میتوکندری و کبد همراه است. هدف از این مطالعه تبیین تأثیر تمرین هوازی تداومی همراه با مصرف بهار نارنج بر بیان ژن‌های HM و MOTS-C میتوکندری بافت کبد رت‌های سالمند بود.
مواد و روش ها: برای انجام تحقیق آزمایشی حاضر 32 سر موش صحرایی ماده سالمند بالای 14 ماه سن و میانگین وزن  270 الی 320 گرم در 4 گروه 8 سری شامل 1- کنترل (CN)، 2- مصرف عصاره بهار نارنج (CA)، 3- تمرین (T)، 4- تمرین و مصرف عصاره بهار نارنج (T+CA) قرار گرفتند. در مدت هشت هفته گروه‌های 3 و 4 به میزان سه جلسه در هفته با شدت 65 تا 75 درصد حداکثر سرعت دویدن روی نوارگردان دویدند؛ همچنین گروه‌های 2 و 4 روزانه mg/kg 300 عصاره CA به صورت صفاقی دریافت نمودند. 48 ساعت پس از پروتکل تحقیق سطوح بیان ژنی در بافت کبد به روش real- time PCR اندازه‌گیری شد.
نتایج: بیان ژن‌های HM و MOTS-C در گروه‌های CA (032/0p= و 042/0p=)، T (039/0p= و 048/0p=) و T+CA (0001/0p= و 001/0p=) نسبت به گروه CN افزایش معنی‌داری داشت.
نتیجه‌گیری: هشت هفته تمرین و مکمل به تنهایی منجر به بهبود پپتیدهای مشتق از میتوکندری در بافت کبد موش‌های صحرایی سالمند شد. همچنین اثر همزمان تمرین  و CA بر HM بیشتر از اثر هر کدام به تنهایی بود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of continuous aerobic exercise with Citrus aurantium L. on the expression of HM and MOTS-C genes in the mitochondria of the liver tissue of aged rats

نویسندگان [English]

  • Saba Ahmadi
  • Saqqa Farajtabar Behrestaq
  • Masoumeh Habibian
  • Babisan Askari
  • Amir Taghipour
Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
چکیده [English]

Background and Objective: Aging process is associated with disruption in mitochondria and liver. The aim of this study was to was to evaluate the effect of continuous aerobic exercise with citrus aurantium L. on the expression of HM and MOTS-C genes in the mitochondria of the liver tissue of elderly rats.
Materials and Methods: To conduct the present experimental research, 32 elderly female rats over 14 months of age and average weight of 270 to 320 grams were divided into 4 groups of 8 including 1- Control (CN), 2- Consumption of citrus aurantium L. (CA), 3- Training (T), 4- Training and consumption of citrus aurantium L. (T+CA). During eight weeks, groups 3 and 4 ran three sessions a week with an intensity of 65-75% of the maximum running speed on the treadmill. Also, groups 2 and 4 received 300 mg/kg of CA extract intraperitoneally. 48 hours after the research protocol, gene expression levels in liver tissue were measured by real-time PCR method.
Results: Expression of HM and MOTS-C genes in CA (p=0.032 and p=0.042), T (p=0.039 and p=0.048) and T+CA (p=0.0001 and p=0.001) groups compared to CN group had a significant increase.
Conclusion: Eight weeks of T and CA alone improved the expression of genes involved in Liver cellular senescence markers of elderly rats. Also, the simultaneous effect of exercise and CA on HM was greater than the effect of each one alone.

کلیدواژه‌ها [English]

  • Training
  • Citrus aurantium
  • Mitochondria
  • HM
  • MOTS-C

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دانشور پزشکی
دوره 32، شماره 3 - شماره پیاپی 172
مرداد و شهریور 1403
صفحه 58-67

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