تاثیر یک دوره تمرین تناوبی با شدت بالا همراه با مصرف غذای پرچرب بر تنظیم کمپلکس اینفلامازومی در بافت چربی موش های سفید بزرگ آزمایشگاهی نر

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

10.22070/daneshmed.2024.17631.1500

چکیده

مقدمه و هدف: کمپلکس اینفلامازومی باعث بلوغ و گسترش فعالیت کاسپاز-1 و سیتوکین­های پیش التهابی از جمله IL-1β می­شود. همچنین برای القای مرگ سلولی پیروپتوتیک در پاسخ به پاتوژن­ها و سیگنال­های خطر درون­زا حیاتی است. از این­رو مطالعه حاضر با هدف بررسی تاثیر یک دوره تمرین تناوبی با شدت بالا همراه با مصرف غذای پرچرب بر تنظیم کمپلکس اینفلامازومی در بافت چربی موش­های صحرایی نر انجام شد.
مواد و روش ها: در مطالعه تجربی حاضر؛ 24 سر موش سفید بزرگ آزمایشگاهی نر ویستار (سن: شش هفته)؛ به چهار گروه کنترل (C)، تمرین (HIIT) ، رژیم پرچرب (HFD)، رژیم پرچرب+تمرین (HFD +HIIT)، تقسیم شدند. مداخلات رژیم غذایی و ورزش همزمان شروع شد و به ­مدت 10 هفته ادامه داشت. وزن، BMI، پروفایل لیپیدی سرم خون و هم­چنین بیان NLRP3 و IL-1β بافت چربی احشایی مورد اندازه­گیری قرار گرفت. برای تعیین تغییرات میان گروه­ها از آنووای یکراهه و آزمون تعقیبی توکی و برای تعیین شدت اندازه اثر از آزمون تی مستقل با استفاده از نرم افزار SPSS27 استفاده شد (05/0P<).
نتایج: کاهش معنی­دار وزن (009/0P=)، بیان NLRP3 (001/0P=)، IL-1β (001/0P=) و میزان LDL و کلسترول (001/0P=) در گروه HFD+HIIT نسبت به HFD دیده شد. همچنین افزایش معنی­دار HDL و HDL/LDL در گروه HFD+HIIT نسبت به گروه HFD (001/0P=) مشاهده شد.
نتیجه‌گیری: پروتکل تمرینی به کار رفته استراتژی درمانی موثری برای بهبود اختلالات متابولیک و وضعیت التهابی است که منجر به کاهش التهاب می­شود. با این­حال برای مشخص نمودن مکانیسم­های سلولی دیگر التهاب به انجام تحقیقات بیشتری نیاز است.  

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of high-intensity interval training with high-fat diet on the regulation of the inflammasome complex in the adipose tissue of male rats

نویسندگان [English]

  • Mojgan Rezvani Rad
  • Mahdieh Molanouri Shamsi
  • Reza Gharakhanlou
  • Shahnaz Shahrbanian
Department of Physical Education & Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Background and Objective: Inflammasome complex causes maturation and expansion of caspase-1activity and pro-inflammatory  cytokines including IL-1β. It is also critical for inducing pyroptotic cell death in response to pathogens and endogenous danger signals. Therefore, the present study was conducted to investigate the effect of high-intensity interval training with high-fat food on the regulation of the inflammasome complex in the adipose tissue of male Wistar rats.
Materials and Methods: In the present experimental study; 24 male Wistar rats (age: six weeks); were divided into four groups: control (C), exercise (HIIT), high-fat diet (HFD), high-fat diet+exercise (HFD+HIIT). Diet and exercise interventions started at the same time and continued for 10 weeks. Weight, BMI, serum lipid profile, and also the expression of NLRP3 and IL-1β in visceral adipose tissue were measured. To determine changes between groups, one-way ANOVA and Tukey's post hoc test were used, and an independent t-test was used to determine the intensity of the effect size using SPSS27 software (P<0.05).
Results: Significant weight reduction (P=0.009), NLRP3 expression (P=0.001), IL-1β (P=0.001), and LDL and cholesterol levels (P=0.001) in the HFD+HIIT group compared to HFD were seen. Also, a significant increase in HDL and HDL/LDL was observed in the HFD+HIIT group compared to the HFD group (P=0.001).
Conclusion: The exercise training protocol is used in the current study as an effective therapeutic strategy for improving metabolic disorders and inflammatory conditions, which leads to the reduction of inflammation. However, more research is needed to determine other cellular mechanisms of inflammation.

کلیدواژه‌ها [English]

  • High-intensity interval training
  • Adipose Tissue
  • Inflammation
  • High-fat diet

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دانشور پزشکی
دوره 32، شماره 3 - شماره پیاپی 172
مرداد و شهریور 1403
صفحه 46-57

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