ارتباط میزان فیلوم باکتریایی شیر با میزان درشت مغذی ها و ریزمغذی های دریافتی در رژیم غذایی مادران شیرده با شاخص توده بدنی بالا در مقایسه با طبیعی: یک مطالعه مورد-شاهدی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه میکروبیولوژی، دانشگاه آزاد اسلامی واحد زنجان، زنجان، ایران

2 گروه تغذیه، دانشکده بهداشت، دانشگاه علوم پزشکی زنجان، زنجان، ایران

3 گروه میکروب شناسی و ویروس شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران

چکیده

مقدمه و هدف: در مطالعه حاضر میزان چهارفیلوم اصلی در شیرمادران با شاخص توده بدنی (BMI) بالا و مادران با BMI طبیعی ارزیابی شده و ارتباط آنها با میزان درشت- و ریز مغذی های دریافتی در رژیم غذایی مورد بررسی قرار گرفته است.
مواد و روش ها: 30 مادر با BMI بالا (بزرگتر-مساوی kg/m230) و 30 مادر با BMI طبیعی (بینkg/m2 5/18-9/24) که در ماه سوم شیردهی بودند از مراکز بهداشتی شهرزنجان انتخاب شدند. نمونه شیر در شرایط کاملا استریل جمع آوری شد. DNA ی باکتریایی استخراج شده و تکثیر ژن 16S rRNA به روش qPCR و با استفاده از پرایمرهای Universal باکتریایی انجام شد. اطلاعات غذایی با استفاده از پرسشنامه بسامدخوراک (FFQ) جمع آوری شده و با ثبت خوراک 3 روزه معتبر شد.
نتایج: با تعدیل اثر همه فاکتورهای مورد بررسی، مادران با BMI طبیعی 41/1 برابر ژنوم اکتینوباکتریای بیشتری در شیر خود داشتند (04/0p=). میزان آهن و ویتامین C دریافتی در رژیم غذایی مادر ارتباط منفی معنی داری با جمعیت اکتینوباکتریا و فرمی کوتس (به ترتیب) در شیر نشان داد (04/0p=، 3/2-OR= و 02/0p=، 96/0- =OR). همچنین میزان کلسترول دریافتی در رژیم غذایی مادر ارتباط معنی دار با جمعیت باکتریوئیدتس نشان داد (04/0p=، 81/0=OR).
نتیجه گیری: حضور ژن اکتینوباکتریا به عنوان فیلوم مفید، در شیرمادران با BMI طبیعی بالاتر از مادران با BMI بالا بود. آهن و ویتامین C دریافتی در رژیم غذایی مادر ارتباط معکوس با میزان اکتینوباکتریا و فرمی کوتس داشت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Relationship between the milk bacterial phyla and the amount of dietary macronutrients and micronutrients intake in lactating mothers with high body mass index compared to the normal: a case-control study

نویسندگان [English]

  • Shahla Karami 1
  • Seyedeh Neda Mousavi 2
  • Reza Shapoury 1
  • Siamak Heidarzadeh 3
  • Davoud Afshar 3
1 Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2 Department of Nutrition, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran
3 Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
چکیده [English]

Objective: In the present study, the amount of the four main phyla in obese lactating mothers and mothers with a normal body mass index was evaluated and their relationship with the amount of macro- and micronutrient’s intake was investigated.
Materials and Methods: 30 mothers with high BMI (greater-equal to 23 kg/m2) and 30 mothers with normal BMI (between 18.5-24.9 kg/m2) who were in the fourth month of breastfeeding were selected from the health centers of Zanjan city. The milk sample was collected under completely sterile conditions. Bacterial DNA was extracted and amplification of 16S rRNA gene was done by qPCR method using universal bacterial primers. Dietary information was collected using a food frequency questionnaire (FFQ) and validated by a three-day food diary.
Results: Adjusting for all parameters, mothers with a normal body mass index had 1.41 times more Actinobacteria gene in milk (p=0.04). The amount of iron and vitamin C intake showed a significant negative relationship with the Actinobacteria and Firmicutes population in milk, respectively (OR=-2.3, p=0.04, OR=-0.96, p=0.02). Also, the amount of dietary cholesterol showed a significant relationship with the Bacteroidetes population (OR=0.81, p=0.04).
Conclusion: Actinobacteria gene presence, as a beneficial phylum, was higher in lactating mothers with normal than the high body mass index. Dietary Iron and vitamin C were inversely related to the Actinobacteria and Firmicutes population.

کلیدواژه‌ها [English]

  • Bacterial phylum
  • Breast milk
  • Body mass index

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دانشور پزشکی
دوره 32، شماره 3 - شماره پیاپی 172
مرداد و شهریور 1403
صفحه 12-23

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