تاثیر هشت هفته تمرین مقاومتی بر محتوای پروتئین‌های RAS و RAF-1 در عضله EDL موش‌های بزرگ آزمایشگاهی پیر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، واحد علی‌آباد کتول، دانشگاه آزاد اسلامی، علی‌آباد کتول، ایران

2 گروه تربیت بدنی و علوم ورزشی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران

10.22070/daneshmed.2024.19018.1487

چکیده

مقدمه و هدف: مسیر پروتئین کیناز فعال‌شده با میتوژن (MAPK)، از مسیرهای مهم در تنظیم حجم عضلانی می‌باشد و پیری از عوامل مهم در اختلال این است؛ بنابراین هدف از انجام تحقیق حاضر، تاثیر هشت هفته تمرین مقاومتی بر محتوای پروتئین‌های RAS وRAF-1 در عضله بازکننده طویل انگشتان پا                         (Extensor Digitorum Longus) موش‌های بزرگ آزمایشگاهی پیر می‌باشد.
مواد و روش ها: در پژوهش تجربی‌بنیادی حاضر، تعداد 12 سر موش بزرگ آزمایشگاهی نر 20 ماهه از نژاد اسپراگ‌داولی با میانگین وزنی 30±400 گرم به‌صورت تصادفی به 2 گروه 1-کنترل (6 سر) و 2-تمرین مقاومتی (6 سر) تقسیم شدند. برنامه تمرین مقاومتی به مدت 8 هفته، هفته‌ای 3 جلسه بود. رت‌ها از یک نردبان عمودی با شیب 85 درجه به طول یک متر با 26 پله و دو سانتی‌متر فضای بین هر پله بالا می‌رفتند. از طریق روش آزمایشگاهی وسترن‌بلات محتوای پروتئین‌ها اندازه‌گیری شد. تجزیه و تحلیل داده‌ها از طریق آزمون t-مستقل در نرم‌افزارهای SPSS نسخه 27 و گراف‌پد پریسم نسخه 2/2/10 انجام شد. سطح معنا‌داری P≤0.05 بود.
نتایج: هشت هفته تمرین مقاومتی منجر به افزایش معنی‌دار میزان پروتئین‌های RAS و RAF-1 در عضله EDL موش‌های بزرگ آزمایشگاهی پیر شد (0001/0P=). آزمون کوهن اندازه اثر قدرتمندی را برای میزان پروتئین‌های RAS (44/4) و RAF-1 (88/4) نشان داد.
نتیجه‌گیری: به نظر می‌رسد افزایش پروتئین‌های RAS و RAF-1 به دنبال انجام تمرین مقاومتی می‌تواند منجر به شروع مسیر MAPK به عنوان یک مسیر در تنظیم حجم و توده عضلانی شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of 8-week resistance training on the content of RAS and RAF-1 proteins in the EDL muscle of old rats

نویسندگان [English]

  • Hamid Khodaverdi 1
  • Neda Aghaei Bahmanbeglou 1
  • Reza Rezaee Shirazi 1
  • Saeedeh Shadmehri 2
1 Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 Department of Physical Education and Sport Science, Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Background and Objective: The mitogen-activated protein kinase (MAPK) pathway is one of the important pathways in the regulation of muscle volume, and aging is one of the important factors in this disorder. Therefore, the aim of this research is the effect of eight weeks of resistance training on the content of RAS and RAF-1 proteins in the extensor digitorum longus (EDL) muscle of old rats.
Materials and Methods: In this fundamental experimental research, 12 male Sprague-Dawley rats aged 20 months with an average weight of 400±30 gr were randomly divided into 2 groups:1-control (6 rats) and 2- resistance training (6 rats). The resistance training program was 3 sessions per week for 8 weeks. Rats climbed a one meter vertical ladder with an 85-degree incline, 26 rungs and two cm of space between each rung. The content of proteins was measured through Western Blot laboratory method. Data analysis was done through independent t-test in SPSS version 27 and GraphPad Prism version 10.2.2. The significance level was set at P≤ 0.05.
Results: Eight weeks of resistance training led to a significant increase in RAS and RAF-1 protein levels in the EDL muscle of aged rats (P=0.0001). Cohen's test showed a strong effect size for the amount of RAS (4.44) and RAF-1 (4.88) proteins.
Conclusion: It seems that the increase of RAS and RAF-1 proteins following resistance training can lead to the initiation of the MAPK pathway as a pathway in the regulation of muscle volume and mass.

کلیدواژه‌ها [English]

  • Resistance training
  • RAS protein
  • RAF-1 protein
  • EDL muscle
  • Aging

مراجع

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دانشور پزشکی
دوره 32، شماره 2 - شماره پیاپی 171
خرداد و تیر 1403
صفحه 63-72

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