نقش هم افزایی تمرین هوازی و مصرف رزوراترول بر مسیر بیوژنز میتوکندری هیپوکامپ موش‌های مبتلا به آلزایمر

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

چکیده

مقدمه و هدف: پاتوژنز بیماری آلزایمر نشان می‌دهد که عدم تعادل بین تولید و پاکسازی آمیلوئید)  β- (Aβعامل توسعه زوال عقل است. فعالیت ورزشی رسوب Aβ را از طریق مسیر سیگنال‌دهی AMPK کاهش می‌دهد. علاوه بر این، رزوراترول (RSV) دارای اثرات محافظت‌کننده عصبی مرتبط با زوال شناختی است. هدف از پژوهش حاضر بررسی اثر تمرین هوازی و مصرف رزوراترول بر مسیر AMPK/PGC-1α/SIRT1 هیپوکامپ موش‌های مبتلا با آلزایمر بود.
مواد و روش ها: در این مطالعه تجربی، 35 سر موش صحرایی نر نژاد ویستار در پنج گروه کنترل (NO)، آلزایمر (AD)، آلزایمر-تمرین (ADT)، آلزایمر-رزوراترول (ADRSV) و آلزایمر-تمرین-رزوراترول (ADTRSV) قرار گرفتند. گروه های مکمل، طی دوره مداخله روزانه 20 میلی‌گرم RSV (به ازای هر کیلوگرم وزن بدن) را به صورت خوراکی دریافت کردند. برنامه تمرین هوازی شامل دویدن روی تردمیل با سرعت 18-6 متر در دقیقه، پنج روز هفته به مدت هشت هفته اجرا شد.
نتایج: القای AD باعث کاهش معنی‌داری در بیان ژن AMPK/PGC-1α/SIRT1 شد (0001/0=p). تمرین و RSV باعث افزایش معنی‌داری بیان ژن AMPK/PGC-1α/SIRT1 در موش‌های صحرایی AD شد (p<0.05). همچنین افزایش معنی‌داری در میزان تغییرات بیان ژن AMPK، PGC-1α و SIRT1 در گروه ADTRSV نسبت به گروه‌ ADT ( به ترتیب 034/0p=، 020/0p= و 038/0p=) و ADRSV (026/0p=، 021/0p= و 021/0p=) مشاهده شد.
نتیجه‌گیری: القای AD با کاهش بیان ژن AMPK/PGC-1α/SIRT1 همراه بود و فعالیت ورزشی هوازی و مصرف RSV قادر است این روند را معکوس کند. به نظر تغییر سطوح این شاخص‌ها به دنبال فعالیت‌بدنی و استفاده از RSV می‌تواند تا حدی عوارض بیماری AD را کاهش دهد.

کلیدواژه‌ها

عنوان مقاله [English]

The synergistic role of aerobic training and resveratrol consumption on the pathway of hippocampal mitochondrial biogenesis in rats with Alzheimer's disease

نویسندگان [English]

  • Ammar Rashet
  • Ahmad Abdi
  • Alireza Barari
Department of Physical Education and Sport Science, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
چکیده [English]

Background and Objective: The pathogenesis of AD shows that the imbalance between the production and clearance of amyloid-β (Aβ) is the cause of the development of dementia. Exercise reduces Aβ deposition through the AMPK signaling pathway. In addition, resveratrol (RSV) has neuroprotective effects associated with cognitive decline. The aim of the present study was to investigate the effect of aerobic training and RSV consumption on the AMPK/PGC-1α/SIRT1 pathway in the hippocampus of rats with Alzheimer's disease.
Materials and Methods: In this experimental study, 35 male Wistar rats were divided into 5 groups: Control (NO), Alzheimer's (AD), Alzheimer's-Training (ADT), Alzheimer's-Resveratrol (ADRSV) and Alzheimer's-Training-Resveratrol (ADTRSV). The supplement groups received 20 mg of RSV (per kg of body weight) orally during the intervention period. Aerobic exercise program including running on treadmill with a speed of 6-18 meters per minute, was performed 5 days a week for eight weeks.
Results: AD induction caused a significant decrease in the gene expression of AMPK/PGC-1α/SIRT1 (p=0.0001). Exercise and RSV significantly increased the gene expression of AMPK/PGC-1α/SIRT1 in AD rats (p<0.05). Also, a significant increase was observed in the gene expression changes of AMPK, PGC-1α and SIRT1 in the ADTRSV group compared to the ADT group (p=0.034, p=0.020 and p=0.038, respectively) and ADRSV (p=0.026, p=0.021 and p=0.021).
Conclusion: AD induction was associated with a decrease in AMPK/PGC-1α/SIRT1 gene expression, and aerobic exercise and RSV consumption can reverse this process. It seems that changing the levels of these indicators following physical activity and the use of RSV can partially reduce the complications of AD.

کلیدواژه‌ها [English]

  • Exercise
  • Resveratrol
  • Mitochondrial biogenesis
  • Alzheimer's disease

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دانشور پزشکی
دوره 32، شماره 2 - شماره پیاپی 171
خرداد و تیر 1403
صفحه 52-62

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