تأثیر تمرین هوازی همراه با مصرف ژل رویال بر بیان BCL-2، Bax و نسبت BCL2‏/Bax بافت پانکراس موش‌های صحرایی چاق

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی وعلوم ورزشی ، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران

چکیده

مقدمه و هدف: چاقی به‌عنوان یک بیماری جهانی در نظر گرفته می‌شود. هدف از انجام این پژوهش، تبیین اثر تمرین هوازی همراه با مصرف ژل رویال بر بیان BCL-2، Bax و نسبت BCL2‏/Bax بافت پانکراس موش‌های صحرایی چاق بود.
مواد و روش ها: 40 سر موش صحرایی نر 5 هفته‌ای ویستار به‌صورت تصادفی به دو گروه رژیم غذایی نرمال (ND) و رژیم غذایی پرچرب (HFD) تقسیم شدند. موش‌های گروه  NDبه مدت 8 هفته با غذای استاندارد (23 درصد پروتئین، 65 درصد کربوهیدرات و 12 درصد چربی) و گروه  HFDاز رژیم غذایی پرچرب (17 درصد پروتئین، 43 درصد کربوهیدرات و 40 درصد چربی) استفاده کردند. سپس موش‌ها به 5 گروه رژیم غذایی نرمال، پرچرب، پرچرب-تمرین (HFDT)، پرچرب- مکمل (HFDRj) و پرچرب-تمرین- مکمل (HFDTRj) تقسیم شدند. گروه تمرین به مدت 8 هفته و 5 روز در هفته تمرین هوازی فزاینده را انجام دادند. همچنین به گروه‌های مکمل، روزانه 100 میلی‌گرم ژل رویال مخلوط‌شده در آب مقطر خورانده شد.
نتایج: نتایج نشان داد القای چاقی از طریق مصرف غذاهای پرچرب موجب افزایش بیان ژن BCL-2 و کاهش معنی‌دار بیان ژن BAX در گروه پرچرب در مقایسه با گروه کنترل شد. همچنین تمرینات استقامتی و مکمل ژل موجب کاهش بیان ژن BCL-2 و افزایش بیان ژن BAX در گروه‌های تجربی در مقایسه با گروه پرچرب شد که این روند تغییرات در گروه ترکیبی محسوس‌تر بود.
نتیجه‌گیری: احتمال دارد تمرینات استقامتی همراه با مصرف ژل رویال بتواند راهبردی مؤثر در بهبود وضعیت چاقی از طریق فرایند آپوپتوز باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of aerobic training with royal jelly on the expression of BCL-2, Bax and Bax / BCL2 ratio in pancreatic tissue of obese rats

نویسندگان [English]

  • Saqqa Farajtabar Behrestaq
  • Mahbobeh Khonakdar
  • Amir Taghipour
  • Babisan Askari
Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.
چکیده [English]

Background and Objective: Obesity is considered as a global disease. The purpose of this study was to evaluate the effect of aerobic exercise with royal jelly on the expression of BCL-2, Bax and the ratio of BCL2/Bax in the pancreatic tissue of obese rats.
Materials and Methods: In this research, 40 5-week-old male Wistar rats were randomly divided into two groups of normal diet (ND) and high-fat diet (HFD). Rats in the ND group were fed a standard diet (23% protein, 65% carbohydrates, and 12% fat) and the HFD group were fed a high-fat diet (17% protein, 43% carbohydrates, and 40% fat) for eight weeks. Then, the rats were divided into 5 groups: normal diet, high-fat diet, high-fat-exercise (HFDT), high-fat-supplemented (HFDRj) and high-fat-exercise-supplemented (HFDTRj) groups. The training group performed incremental aerobic training for 8 weeks and 5 days per week. Also, the supplement groups were daily given 100 mg of royal jelly mixed in distilled water.
Results: The results showed that induction of obesity through the consumption of high-fat foods increases BCL-2 gene expression and significantly decreases BAX gene expression in the high-fat group compared to the control group. Also, endurance exercises and gel supplementation decreased BCL-2 gene expression and increased BAX gene expression in the experimental groups compared to the high-fat group and these changes were more noticeable in the combined group.
Conclusion: It is possible that aerobic training along with the use of royal jelly can be an effective strategy in improving the condition of obesity through the process of apoptosis.

کلیدواژه‌ها [English]

  • Endurance training
  • Royal jelly
  • BAX
  • BCL-2
  • obesity

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دانشور پزشکی
دوره 31، شماره 6 - شماره پیاپی 169
بهمن و اسفند 1402
صفحه 26-36

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