تجزیه و تحلیل متابولومیکی عضله اسکلتی پس از تمرین تناوبی شدید در موش‌های تغذیه‌شده با رژیم غذایی پرچرب

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

3 مرکز تحقیقات سلولی و مولکولی غدد، پژوهشکده علوم غدد درون ریز، دانشگاه علوم پزشکی شهید بهشتی، تهران

چکیده

مقدمه و هدف: رژیم غذایی پرچرب (HFD)، باعث اختلال در مسیرهای تولید انرژی شده و احتمال بروز مقاومت به انسولین (IR) را افزایش می‌دهد. هدف از تحقیق حاضر بررسی اثر تمرین تناوبی شدید (HIIT) بر نیم‌رخ متابولیکی مرتبط با بروز IR در موش‌های نر تغذیه‌شده با HFD و رژیم غذایی استاندارد (SD) بود.
مواد و روش ها: 32 سر موش صحرایی نر (سن 8-7 هفته) به چهار گروه مساوی شامل HIIT+HFD، HIIT+SD، کنترل دریافت‌کننده HFD (HFD control) و کنترل دریافت‌کننده SD (SD control) تقسیم شدند. سپس مداخله‌هایHIIT  (12 هفته، پنج جلسه در هفته با شدت 90-85 درصد حداکثر اکسیژن مصرفی) و رژیم غذایی اعمال شد و درنهایت، عضله دوقلو به‌منظور بررسی بافتی به‌روش کروماتوگرافی گازی-طیف‌سنجی جرمی برداشته شد. آنالیز داده‌ها با استفاده از آزمون‌های آماری آنالیز واریانس یک‌طرفه، آنالیز کوواریانس یک‌طرفه و کروسکال-والیس در سطح معناداری 05/0> P  انجام شد..
نتایج: میزان اسیدسیتریک عضله دوقلو در گروه  HFD control نسبت به گروه control SD به‌طور معناداری پایین‌تر بود. علاوه‌براین، میزان اسیدسیتریک در گروه‌های HIIT+SD و HIIT+HFD نسبت به گروه HFD control به‌طور معناداری بالاتر بود. بین میزان متابولیت‌های مالات، گلوکز 6-فسفات، ریبوز، گزیلوز و ریبولوز 5-فسفات در بین گروه‌ها تفاوت معناداری مشاهده نشد. همچنین، وزن بدن در گروه‌های HIIT+SD و HIIT+HFD در مقایسه با گروه HFD control به‌طور معناداری پایین‌تر بود.
نتیجه‌گیری: HFD از طریق کاهش میزان اسیدسیتریک عضلانی به‌عنوان واسطه متابولیتی مهم چرخه TCA، در تأمین انرژی عضلات اسکلتی از مسیر هوازی محدودیت ایجاد می‌کند. انجام HIIT می‌تواند به‌واسطه افزایش میزان اسیدسیتریک و کاهش شیب افزایش وزن بدن، در کاهش اختلالات متابولیکی ناشی از HFD مؤثر واقع شود.

کلیدواژه‌ها

موضوعات

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