کاهش سطح TNF-α تولید‌شده از سلول‌های تک‌هسته‌ای خون محیطی بیماران مبتلا به کووید-19 در اثر تیمار با عصاره اتانولی بابونه

نوع مقاله : مقاله پژوهشی

نویسندگان

مرکز تحقیقات تنظیم پاسخ‌های ایمنی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: سازمان بهداشت جهانی در مارس 2020 بیماری کروناویروس 2019 را به‌عنوان یک بیماری همه‌گیر معرفی کرد. MERS-CoV و آنفولانزا می‌توانند منبع ارزشمندی در توسعه عوامل درمانی COVID-19 باشند. Matricaria chamomilla L معمولاً به دلیل خواص ضدالتهابی و ضدباکتریایی مفید است و در فارماکوپه 26 کشور به‌عنوان یک داروی گیاهی برای درمان اختلالات مختلف ذکر شده است. این مطالعه به‌منظور ارزیابی پتانسیل بابونه برای کاهش سطح سایتوکاین پیش‌التهابی TNF-α انجام شد.
مواد و روش ها: سلول‌های تک‌هسته‌ای خون محیطی (PBMCs) از داوطلبان کووید-19 (3 نفر) جداسازی و با غلظت‌های 200، 300، 400، 500 میکروگرم بر میلی‌لیتر عصاره اتانولی که از اندام‌های هوایی بابونه استخراج شده بود تیمار شدند. سطوح تولید فاکتور نکروز تومور آلفا (TNF-α) با استفاده از روش الایزا اندازه‌گیری شد.
نتایج: عصاره اتانولی بابونه (تمام غلظت‌ها) تولید سایتوکاین پیش‌التهابی TNF-α را در کشت سلول‌های خون محیطی بیماران کووید-19 به‌طور قابل توجهی نسبت ‌به گروه تیمارنشده کاهش داد (0001/0>p).
نتیجه‌گیری: نتایج مطالعه حاضر برای نخستین‌بار نشان می‌دهد که بابونه می‌تواند از طریق کاهش سطح سایتوکاین پیش‌التهابی TNF-α اثرات ضدالتهابی خود را اعمال کند که این اثرات را می‌توان به ترکیبات این گیاه با خواص ضدالتهابی نسبت داد. این گیاه به‌عنوان جایگزینی بالقوه برای درمان بیماری‌های التهابی نیاز به بررسی و مطالعات بیشتر دارد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Reduction of TNF-a level produced by peripheral blood leukocytes of patients with COVID-19 due to treatment with chamomile ethanolic extract

نویسندگان [English]

  • Rasoul Rashidi
  • Tayabeh Radjabian
  • Tooba Ghazanfari
Immunoregulation Research Center, Shahed University, Tehran, Iran
چکیده [English]

Background and Objective: In March 2020, World Health Organization (WHO) declared 2019 coronavirus disease as a pandemic. MERS-CoV and influenza can be a valuable resource in the development of therapeutic agents for COVID-19. Matricaria chamomilla L. (chamomile) is usually useful due to its anti-inflammatory and antibacterial properties and is listed in the pharmacopoeia of 26 countries as a herbal medicine for the treatment of various disorders. This study was conducted to evaluate the potential of chamomile to reduce the level of pro-inflammatory cytokine TNF-α.
Materials and Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from Covid-19 volunteers (n = 3) and treated with concentrations of 200, 300, 400, and 500 µg/ml ethanolic extract extracted from chamomile aerial parts. Tumor necrosis factor alpha (TNF-α) production levels were measured using ELISA method.
Results: Chamomile ethanolic extract (all concentrations) significantly reduced the production of pro-inflammatory cytokine TNF-α in peripheral blood cell cultures of covid-19 patients compared to the untreated group (p<0.0001).
Conclusion: The results of the present study showed for the first time that chamomile can exert its anti-inflammatory effects by reducing the level of the pro-inflammatory cytokine TNF-α, which can be attributed to the compounds of this plant with anti-inflammatory properties. As a potential alternative for the treatment of inflammatory diseases, this plant needs further investigation.

کلیدواژه‌ها [English]

  • Chamomile
  • ethanolic extract
  • COVID-19
  • PBMCs
  • tumor necrosis factor alpha (TNF-α)

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دانشور پزشکی
دوره 31، شماره 4 - شماره پیاپی 167
مهر و آبان 1402
صفحه 1-10

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