تاثیر یک دوره تمرین تناوبی پر شدت بر بیان ژن فاکتور رشد تغییردهنده بتا (TGF-β1) و اندازه سلول ها در بافت چربی زیر جلدی رت های تغذیه شده با غذای پرچرب

نوع مقاله : مقاله پژوهشی


1 گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

2 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی و تندرستی، دانشگاه تهران، تهران، ایران


مقدمه و هدف: تأثیر ورزش در کاهش بافت و اندازه سلول چربی احتمال دارد با سیگنال‌دهی TGF-β1 مرتبط باشد. این مطالعه با هدف بررسی تأثیر تمرین تناوبی پرشدت (HIIT) هم‌زمان با تغذیه پرچرب بر بیان ژن TGF-β1 و اندازه سلول‌ها در بافت چربی زیرجلدی رت‌ها انجام شد.
مواد و روش ها: 24 سر موش صحرایی نر بالغ به‌طور تصادفی به 4 گروه ND (تغذیه معمولی)، HFD (تغذیه پرچرب)، HIIT+ND و HIIT+HFT تقسیم شدند. HIIT هشت دوره فعالیت شدید در90 درصد حداکثر ظرفیت دویدن (MRC) به مدت 5/2 دقیقه، با دوره‌های استراحت فعال در50 درصد حداکثر ظرفیت دویدن به مدت 5/2 دقیقه به مدت 10 هفته بود. 48 ساعت پس از آخرین جلسه تمرین، خون‌گیری انجام و چربی زیرجلدی برداشته شد. برای سنجش بیان ژن TGF-β1 از روش qRT-PCR و برای تعیین اندازه سلول‌های چربی از روش استریولوژی استفاده شد. شاخص‌های آنتروپومتریک و متابولیک رت‌ها نیز اندازه‌گیری شد. برای تحلیل داده‌ها از آزمون آنوا دوطرفه با سطح معنا‌داری 05/0≥ P، استفاده شد.
نتایج: تعاملHIIT و رژیم غذایی پرچرب باعث کاهش معنا‌دار بیان ژن TGF-β1(001/0>P، 99/24=F) شد ولی بر اندازه سلول چربی تأثیر معنا‌دار نداشت (780/0=P، 07/0=F). بین بیان ژن TGF-β1و اندازه سلول‌های چربی همبستگی مثبت و معنادار مشاهده شد (001/0>P، 896/0=r).
نتیجه‌گیری: HIIT هم‌زمان با مصرف رژیم غذایی پرچرب از افزایش TGF-β1 در بافت چربی زیرجلدی جلوگیری می‌کند و می‌تواند اثر پیشگیرانه بر افزایش اندازه سلول چربی داشته باشد.



عنوان مقاله [English]

The effect of a period of high-intensity interval training on gene expression of transforming growth factor-beta (TGF-β1) and the size of cells in subcutaneous adipose tissue of rats fed with high-fat diet

نویسندگان [English]

  • Shadi Fayyazi 1
  • Reza Gharakhanlou 1
  • Mahdieh Molanouri 1
  • Mohammadreza Kordi 2
1 Department of Sports Physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, IranModares University, Tehran, Iran
2 Department of Sport Physiology, Faculty of Sports and Health Sciences, University of Tehran, Tehran, Iran
چکیده [English]

Background and Objective: The effect of exercise in reducing fat tissue and fat cell size may be related to TGF-β1 signaling. This study aimed to investigate the effect of high-intensity interval training (HIIT) simultaneously with high-fat diet on TGF-β1 gene expression and cell size in the subcutaneous adipose tissue of rats.
Materials and Methods: In this study, 24 adult male rats were randomly divided into 4 groups: ND (normal diet), HFD (high fat diet), HIIT+ND and HIIT+HFT. HIIT consisted of eight bouts of intense activity at 90% of maximum running capacity (MRC) for 2.5 minutes, with active rest periods at 50% of maximum running capacity for 2.5 minutes for 10 weeks. 48 hours after the last training session, blood was taken and subcutaneous fat was removed. qRT-PCR method was used to measure TGF-β1 gene expression and the stereological method was used to determine the size of fat cells. Two-way ANOVA test with a significant level of P < 0.05 was used to analyze the data.
Results: The interaction between HIIT and high-fat diet caused a significant decrease in TGF-β1 gene expression (F=24.99, P<0.001), but it had no significant effect on fat cell size (P=0.780, F=0.07). A positive and significant correlation was observed between TGF-β1 gene expression and fat cell size (r=0.896, P<0.001).
Conclusion: HIIT at the same time with consuming a high-fat diet prevents the increase of TGF-β1 in the subcutaneous fat tissue and may have a preventive effect on the increase in fat cell size.

کلیدواژه‌ها [English]

  • High-intensity interval training
  • TGF-β1
  • Fat cell size
  • High-fat diet
  • Subcutaneous adipose tissue
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