دانشور پزشکی

دانشور پزشکی

مقایسه اثر دو شیوه تمرین اختیاری در محیط غنی سازی شده و تمرین اجباری بر بیان Wnt-5a و تجمع آمیلوئید بتا در بافت هیپوکامپ موشهای مبتلا به دیابت نوع 3

نوع مقاله : مقاله پژوهشی

نویسندگان
ساناز زنجانیان 1
محمد رضا کردی 2
علی اصغر رواسی 2
1 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، پردیس البرز دانشگاه تهران، تهران، ایران
2 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران
10.22070/daneshmed.2022.16470.1233
چکیده
مقدمه و هدف: دیابت نوع 3 یا دیابت مغزی اصطلاحی است برای بیماری آلزایمر که مقاومت انسولینی مغز را به طور انتخابی درگیر وسبب کاهش شناخت وحافظه می شود. مسیر سیگنالینگ Wnt  در سیستم عصبی، کاهش علائم بیماری آلزایمر، سنتز انسولین و دیابت نقش دارد. اگرچه تأثیر مثبت فعالیتهای بدنی در بیماری‌ها گزارش شده است، اما مقایسه نوع تمرینات کمتر مورد بررسی قرار گرفته است. هدف از پژوهش حاضر مقایسه اثر تمرین اختیاری در محیط غنی سازی شده و تمرین اجباری بر بیان پروتئین Wnt-5a و آمیلوئیدبتا (Aβ) در بافت هیپوکامپ موشهای دیابت نوع 3 می‌باشد.
مواد و روش ها: 25 سر موش به 5 گروه کنترل سالم، شم، دیابت 3، دیابت 3 + تمرین اختیاری در محیط غنی و دیابت 3 + تمرین اجباری تقسیم شدند. القای دیابت 3 با تزریق استروپتوزوسین داخل بطنی و تائید مدل با آزمون رفتاری صورت گرفت. پروتکل تمرینی طی هشت هفته اجرا شد. بیان پروتئین  Wnt-5aبه روش وسترن بلات و تجمع Aβ با رنگ آمیزی تیوفلاوین S انجام شد. تحلیل داده با آزمون آنالیز واریانس یک­ سویه وآزمون تعقیبی توکی صورت گرفت (05/0P≤).
نتایج: تمرین اجباری و اختیاری سبب افزایش معنادار در بیان پروتئین Wnt-5a شد (05/0P≤). در مقایسه گروههای تمرینی، Wnt-5a در موشهای محیط غنی افزایش بیشتری داشت. تجمع Aβ در هر دو گروه تمرینی در مقایسه با گروه دیابت 3 کاهش معناداری یافت (05/0P≤) که این کاهش در محیط غنی بیشتر بود.
نتیجه‌گیری: تمرینات اجباری و اختیاری با فعال کردن مسیر سیگنالینگ Wnt/β-catenin در مغز باعث کاهش تجمع پلاکهای Aβ گردید. به نظر می‌رسد محیط غنی تأثیر بهتری در کاهش علائم مبتلایان دیابت نوع 3 در مقایسه با تمرین اجباری دارد که احتمالاً ناشی از کاهش عوامل استرس‌زا بر مغز باشد.
کلیدواژه‌ها
دیابت نوع 3
تمرین تناوبی شدید
غنی سازی محیط
Wnt-5a
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