اثر تمرین هوازی و ژل رویال بر برخی شاخص‌های آپوپتوز بافت قلب موش‌های چاق

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

چکیده

مقدمه و هدف: چاقی با بی‌نظمی چربی همراه بوده و منجر به افزایش سطوح تری‌گلیسرید، آپوپتوز و اختلال در عملکرد قلب می‌شود. هدف از پژوهش حاضر بررسی اثر تمرین هوازی (AT) ژل رویال (RJ) بر شاخص‌های آپوپتوز سلول‌های قلبی موش‌های صحرایی چاق ناشی از رژیم غذایی پرچرب بود.
مواد و روش ها: در این مطالعه تجربی، 45 موش صحرایی نر به طور تصادفی به پنج گروه (9=n): رژیم غذایی نرمال (ND)، رژیم غذایی پرچرب (HFD)، رژیم غذایی پرچرب+تمرین (HFDT)، رژیم غذایی پرچرب+ژل رویال (HFDRJ) و رژیم غذایی پرچرب+تمرین+ژل رویال (HFDTRJ) تقسیم شدند. گروه‌های مکمل، طی دوره مداخله روزانه 100 میلی‌گرم RJ (به ازای هر کیلوگرم وزن بدن) رقیق شده در آب مقطر را به صورت خوراکی دریافت کردند. برنامه تمرین هوازی شامل دویدن روی تردمیل با شدت 60-50 درصد اکسیژن مصرفی (VO2max)، پنج روز در هفته به مدت هشت هفته بود. داده‌ها با استفاده از ANOVA در سطح معنی‌‌داری 05/0>p مورد بررسی قرار گرفت.
نتایج:  HFDباعث کاهش Bcl2 (006/0=p)، افزایش BAX (006/0=p) و BAX/Bcl2 (006/0=p) شد. AT، RJ و همچنین ترکیب AT با RJ باعث افزایش Bcl2 و کاهش BAX و BAX/Bcl2 شد (05/0p≤). افزایش Bcl2 و کاهش BAX و BAX/Bcl2 در گروه HFDTRJ نسبت به گروه HFDT و HFDRJ معنی‌دار بود (05/0p≤).
نتیجه‌گیری: ترکیب AT و RJ ممکن است با تاثیر بر بیان BAX و Bcl2 باعث بهبود شاخص‌های آپوپتوز در سلول‌های قلبی شده و آسیب قلبی ناشی از HFD را بر مسیر آپوپتوز کنترل کند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of aerobic training and royal jelly on some factors of cardiac apoptosis in obese rats

نویسندگان [English]

  • Mostafa Kazemi
  • Seyedeh Fatemeh Hosseini
  • Ghasem Torabi Palat Kaleh
Department of Physical Education and Sport Science, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
چکیده [English]

Background and Objective: Obesity is associated with dyslipidemia, which leads to elevated triglyceride levels, apoptosis and compromised cardiac function. The aim of this study was to investigate the effect of aerobic training (AT) and royal jelly (RJ) on apoptosis indices in cardiomyocytes in high-fat diet-induced obese rats.
Materials and Methods: In this experimental study, 45 male rats were randomly divided into five groups (n=9): Normal Diet (ND), High-Fat Diet (HFD), High-Fat Diet+Training (HFDT), High-Fat Diet+Royal Jelly (HFDRJ), and High-Fat Diet+Training+Royal Jelly (HFDTRJ). The supplement groups received 100 mg of royal jelly (kg/body weight) diluted in distilled water orally during the intervention period. The aerobic exercise program included treadmill running with an intensity of 50-60% oxygen consumption (VO2max), 5 days/week for 8 weeks. Data were analyzed using ANOVA at p<0.05.
Results: HFD decreased Bcl2 (p=0.006), increased BAX (p=0.006) and BAX/Bcl2 (p=0.006). AT, RJ as well as the combination of AT with RJ increased Bcl2 and decreased BAX and BAX/Bcl2 (p≤0.05). Increase in Bcl2 and decrease in BAX and BAX/Bcl2 were significant in HFDTRJ group compared to HFDT and HFDRJ groups (p≤0.05).
Conclusion: Combining AT with RJ may improve apoptotic markers in cardiomyocytes by affecting BAX and Bcl2 expression and control HFD-induced cardiomyocytes damage on the apoptotic pathway.

کلیدواژه‌ها [English]

  • Exercise
  • Heart
  • Obesity
  • Royal Jelly
  • BAX and Bcl2

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