ارزیابی روشهای مختلف تشخیص تولید بیوفیلم در جدایه های بالینی استافیلوکوکوس اورئوس

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه میکروب شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد قم، قم، ایران

چکیده

مقدمه و هدف: توانایی استافیلوکوکوس اورئوس برای تولید بیوفیلم، به عنوان یک عامل مهم در بیماری‌زایی، پایداری آن را در محیط و میزبان زنده افزایش می دهد. بیوفیلم‌های تشکیل شده توسط استافیلوکوکوس اورئوس، به ویژه آنهایی که با ایمپلنت دستگاههای پزشکی مرتبط هستند، می توانند به عنوان یک مانع فیزیکی در برابر آنتی بیوتیک ها و سیستم ایمنی میزبان عمل کرده، منجر به عفونت های مزمن یا پایدار شوند.  از این رو، انجام آزمایش های تشخیصی کارآمد برای شناسایی تولید بیوفیلم، می تواند به کاهش معضل بیماری کمک کند.  هدف از این مطالعه، ارزیابی روش های مختلف تشخیص تشکیل بیوفیلم در 40 جدایه بالینی استافیلوکوکوس اورئوس بود.
مواد و روش ها: در مجموع 40 جدایه بالینی غیر تکراری استافیلوکوکوس اورئوس، شناسایی شدند. تشکیل بیوفیلم با استفاده از روش های صفحه کشت بافت (TCP)، روش لوله (TM) و کنگو رد آگار (CRA) بررسی شد و برای تشخیص ژن های icaA و icaD از آزمایش  PCR استفاده گردید.
نتایج: در میان جدایه های استافیلوکوکوس اورئوس، 21 (5/52%) جدایه دارای هر دو ژن  icaA و icaD بودند و ژن icaD در تمام جدایه های بیوفیلم مثبت حضور داشت. صفحه کشت بافت کنگو رد آگار و روش لوله، به ترتیب 30% ، 5/42% و 5/67% از جدایه ها را تولید کننده بیوفیلم شناسایی کردند.
نتیجه‌گیری: طبق نتایج، روش صفحه کشت بافت به همراه گلوکز، بهترین همبستگی را با نتایج آنالیز مولکولی نشان داد و می‌تواند به عنوان یک روش قابل اعتماد برای تشخیص تشکیل بیوفیلم در جدایه های بالینی استافیلوکوکوس اورئوس بکار رود.

کلیدواژه‌ها

عنوان مقاله [English]

Assessment of various detection methods of biofilm formation in Staphylococcus aureus clinical isolates

نویسندگان [English]

  • Mozhgan Pirmoradian
  • Mohsen Zargar
  • Razieh Nazari
Department of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran
چکیده [English]

Background and Objective: As an important factor in pathogenicity, the ability of Staphylococcus aureus to produce biofilm increases its stability in the environment and living host. Biofilms formed by S.aureus, especially those related to the implant of medical devices, can act as a physical barrier against antibiotics and the host's immune system, leading to chronic or persistent infections. Hence, implementation of efficient diagnostic tests for the detection of biofilm formation can help reduce the disease burden. The purpose of this study was to assess different methods for the detection of biofilm formation in 40 S.aureus isolates.
Materials and Methods: A total of 40 non-duplicate S. aureus clinical isolates were identified. Biofilm formation was detected by Tissue Culture Plate (TCP), tube method (TM) and Congo red agar (CRA) methods and PCR assay was used to detect icaA and icaD genes.
 
Results: Among all S. aureus isolates, 21(52.5%) contained both icaA and icaD genes and icaD gene was present in all biofilm positive isolates. Tissue culture plate, Congo red agar, and tube method detected 30%, 42.5%, and 67.5% biofilm formation isolates, respectively.
Conclusion: According to the results, tissue culture plate with supplemented glucose showed the best correlation with the results of molecular assay and can be used as a reliable method to detect biofilm formation in clinical isolates of S. aureus.

کلیدواژه‌ها [English]

  • Staphylococcus aureus
  • Biofilm
  • PCR technique
  • Ica genes

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