طراحی یک سازه جدید واکسن مولتی اپی توپ پپتیدی علیه SARS-CoV-2 با استفاده از ابزار ایمونوانفورماتیک

نوع مقاله : مقاله پژوهشی

نویسنده

گروه بیوتکنولوژی پزشکی، دانشکده علوم نوین پزشکی، دانشگاه علوم پزشکی فسا، فسا، فارس، ایران

چکیده

مقدمه و هدف: ویروس سندرم تنفسی حاد کرونا ویروس (SARS-CoV-2) برای اولین بار در چین ظهور پیدا کرد و به سرعت در سراسر جهان منتشر شد. به دلیل شیوع گسترده آن در سراسر جهان تلاش برای ارائه واکسن موثر و ایمن علیه این ویروس ضروری می باشد. ژنوم این ویروس دارای مولکول RNA تک رشته ای می‌باشد که کدکننده چهار پروتئین ساختاری متفاوت می‌باشد، از میان آنها، پروتئین های اسپایک (S) و نوکلئوکپسید ویروس (N) نقش بسزایی در تحریک سیستم ایمنی در جهت مقابله با ویروس را دارند. واکسن های مولتی اپی توپ پپتیدی که شامل اپی توپ های ایمنی زای سلول های T و B هستند، به دلیل اختصاصیت بالا در سال های اخیر بسیار مورد توجه واقع شده اند. این واکسن ها با استفاده از ابزار ایمونوانفورماتیک طراحی می‌شوند.
مواد و روش ها: در این پژوهش پروتئین های S و N  SARS-CoV-2 با کمک سرورهای بیوانفورماتیک به منظور شناسایی اپی توپ‌های سلول های T CD4 و B آنالیز شدند. از زیر واحد B توکسین کلرا و اپی توپ PADRE به عنوان ادجوانت استفاده شد. اجزای مذکور با لینکرهای پپتیدی به هم متصل شدند و ویژگی های سازه واکسن طراحی شده از جمله آنتی ژنیسیته، عدم آلرژنیسیته، خصوصیات فیزیکی و شیمیایی، ساختار دوم و سوم با استفاده از سرورهای بیوانفورماتیک پیشگویی شد.
نتایج: براساس نتایج آنالیزهای بیوانفورماتیک سازه مذکور دارای آنتی ژنیسیته بالا بوده و آلرژن نیز نمی‌باشد.
نتیجه‌گیری: بنابراین سازه طراحی شده به عنوان یک کاندید واکسن مناسب علیه            SARS-CoV-2 می‌تواند مورد بررسی قرار گیرد، هر چند انجام مطالعات آزمایشگاهی ضروری می‌باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Designing a novel multi-epitope peptide vaccine against SARS-Cov-2 using immunoinformatics tool

نویسنده [English]

  • Shirin Mahmoodi
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran
چکیده [English]

Background and Objective: Acute Coronavirus Syndrome Virus (SARS-CoV-2) virus first appeared in China and spread rapidly around the world. Due to its wide spread around the world, efforts are needed to provide an effective and safe vaccine against this virus. The virus genome contains a single-stranded RNA molecule that encodes four different structural proteins, among which the virus spike (S) and nucleocapsid (N) proteins play an important role in stimulating the immune system to fight the virus. Multi-epitope peptide vaccines, which include immunogenic epitopes of T and B cells, have received much attention in recent years due to their high specificity. These vaccines were designed using immunoinformatics tools.
Materials and Methods: In this study, the S and N proteins of SARS-CoV-2 were analyzed with the help of bioinformatics servers to identify CD4 and B T cell epitopes. Cholera toxin B subunit and PADRE epitope were used as adjuvants. The components were linked together by peptide linkers and the structural features of the vaccine were predicted, including antigenicity, non-allergenicity, physicochemical properties, secondary and tertiary structures using bioinformatics servers.
Results: According to the results of bioinformatics analysis, the structure has high antigenicity and is not allergenic.
Conclusion: Therefore, the designed structure as a suitable vaccine candidate against SARS-CoV-2 can be examined, although experimental studies are necessary.

کلیدواژه‌ها [English]

  • Acute coronavirus respiratory syndrome virus
  • Vaccine
  • Immunoinformatics
  • Spike
  • Nucleocapsid

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دانشور پزشکی
دوره 30، شماره 4 - شماره پیاپی 161
مهر و آبان 1401
صفحه 23-35

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