تأثیر تمرین تناوبی سرعتی بر آنزیم‌های کبدی، ظرفیت آنتی‌اکسیدانی تام و مالون دی آلدئید بافت کبدی موش بزرگ آزمایشگاهی نر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه شاهد، تهران، ایران

2 مرکز تحقیقات نوروفیزیولوژی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: هدف از مطالعه حاضر بررسی تأثیر تمرین تناوبی سرعتی (SIT) بر میزان آنزیم‌های کبدی، ظرفیت آنتی اکسیدانی تام (TAC) و ماالوان-دی-آلدهید (MDA) بافت کبدی بود.
مواد و روش ها: در یک کارآزمایی تجربی، 16 سر موش بزرگ آزمایشگاهی نر (موش) تصادفی به دو گروه کنترل (8=CO, n) و تمرین(8= EX, n) تقسیم شدند. موش‌ها پس از دوران سازگاری با محیط و آشنایی با تمرین، 8 هفته، هفته‌ای 3 جلسه تحت تمرین قرار گرفتند. در پایان دوره، پس از بیهوشی و خونگیری مستقیم از قلب، سرم برای سنجش‌های آنزیم‌های کبدی استخراج و بافت کبد جهت بررسی میزان TAC و MDA بافتی برداشته شد. برای تحلیل داده‌ها از آزمون آماری t مستقل در سطح آلفای 05/0 در محیط SPSS نسخه 22 استفاده شد.
نتایج: تحلیل آماری داده­ها حاکی از بالاتر بودن معنی­دار میزان TAC بافت کبد گروه EX نسبت به گروه CO  بود (01/0=P)؛ اما تفاوت  معنی­دار بین­گروهی در رابطه با  میزان MDA بافتی کبد مشاهده نشد (24/0=P). در رابطه با میزان آنزیم­های کبدی ALT (26/0=P) و GGT (44/0=P) نیز تفاوت بین­گروهی معنی­داری مشاهده نشد؛ اما میزان آنزیم  AST (01/0=P) و (001/0=P) ALP گروه EX نسبت به گروه CO به طور معنی­داری کمتر بود.
نتیجه‌گیری: نتایج نشان داد، تمرین SIT باعث افزایش ظرفیت آنتی اکسیدانی و تغییرات مطلوب در میزان آنزیم‌های کبدی می‌شود و این نوع پروتکل تمرینی می‌تواند پیامد مفیدی بر وضعیت سلامتی وعملکردی کبد داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of sprint interval training on plasma liver enzymes, liver tissue total-antioxidant-capacity and malondialdehyde in male rats

نویسندگان [English]

  • Mohammadhasan Ahmadi 1
  • Mohammad Rahmani 1
  • Mohsen Khalili Najafabadi 2
1 Department of exercise Sciences, Faculty of Humanities, Shahed University, Tehran, Iran
2 Neurophysiology Research Center, Shahed University, Tehran, Iran
چکیده [English]

Objective: The aim of this study was to evaluate the effect of Sprint Interval Training (SIT) on the plasma liver enzymes, liver tissue total-antioxidant-capacity (TAC) and Malondialdehyde (MDA) levels in male rats.
Materials and Methods: In an experimental trial, 16 male rats were randomly divided into two groups: control (CO,n=8) and exercise (EX,n=8). The rats after the period of adaptation to the environment and familiarization with exercise were trained 3 sessions per week for 8 weeks. Finally, after anesthesia and direct blood sampling from the heart, serum was extracted for liver enzymes and liver tissue was removed to assess tissue TAC and MDA. Independent t-test was used to analyze the data at the alpha level of 0.05 in SPSS version 22.
Results: Statistical analysis of the data showed a significantly higher liver tissue TAC in the EX group compared to the CO group (P=0.01); but no significant intergroup difference was observed related to liver tissue MDA (P=0.24). There was no significant difference between the groups in the ALT (P=0.26) and GGT (P=0.44) level; but the AST (P=0.01) and ALP (P=0.001) level in EX group was significantly lower than CO group.
Conclusion: The results showed that SIT increases the antioxidant capacity and induces desirable changes in the liver enzymes levels and this training protocol can have a beneficial effect on the health status of the liver.

کلیدواژه‌ها [English]

  • Malondialdehyde (MDA)
  • Total Antioxidant Capacity (TAC)
  • Sprint Interval Training (SIT)
  • Liver enzymes

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دانشور پزشکی
دوره 30، شماره 2 - شماره پیاپی 159
خرداد و تیر 1401
صفحه 50-60

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