بررسی تمایز سلولهای بنیادی مغز استخوان به نورونها بر روی داربست های نانو ماگنتیک ژلاتین پلی لاکتیک کوگلیکولیک اسید سنتز شده با دو روش ریخته گری انجمادی و خشک انجمادی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه آناتومی، دانشکده پزشکی، دانشگاه علوم پزشکی البرز، کرج، ایران

چکیده

مقدمه و هدف: تمایز سلولهای بنیادین مزانشیمی مغز استخوان                                 BMMSC (bone marrow mesenchymal stem cell) به رده های مختلف سلولی بررسی شده است. سیگنال های مختلف بیوشیمیایی و توپوگرافیک می توانند در تکثیر و تمایز سلولهای بنیادین به یک رده سلولی خاص تأثیر بگذارند. هدف از این مطالعه ارزیابی توانایی BMMSC برای تمایز به سلولهای شبه نورونی است.
مواد و روش ها: از داربست ریخته گری انجمادی و خشک انجمادی  ژلاتین/ پلی لاکتیک-کو-گلیکولیک اسید (PLGA) با نانوذرات آهن (MNPs) و بدون نانوذرات آهن (MNPs-free)  به عنوان یک ساختار توپوگرافی سه بعدی استفاده شد. تمایز BMMSC  به سلولهای شبه نورون تحت اثر محیط های نوروژنیک به مدت 20 روز انجام شد. میزان تکثیر BMMSC  با تست MTT  و بیان مارکرهای خاص نورون با روش ایمونوهیستوشیمی بررسی شد.  در این مطالعه،  روش آماری آنالیز واریانس یک طرفه ANOVA، Post Hock Tukey و آزمون نان پارامتریک Mann-Whitney استفاده شد.
نتایج: داربست ریخته گری انجمادی  ژلاتین-PLGA- NPs حاوی نانوذرات آهن ساختار مناسبی برای تمایز به سلولهای شبه نورونی فراهم می کند. نتایج بقا سلولی (میانگین 7600/0 و انحراف معیار 02000/0 بعد از 72 ساعت) و بیان ژنها (میانگین 00/79 و انحراف معیار 000/1 ) نشان می دهد که میزان تکثیر سلول ها بر روی داربستهای فریز کست شده حاوی نانوذرات بیشتر از داربستهای فریزداری شده می باشد (001/0>P).
نتیجه‌گیری: داربست ریخته گری انجمادی ژلاتین-PLGA حاوی نانوذرات آهن می تواند انتخاب مناسبی برای ترمیم و بازسازی سیستم عصبی باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation of bone marrow stem cells differentiation to neurons on magnetic nanoparticles gelatin/polylactic-co-glycolic acid scaffolds by freezed casting and freezed drying methods

نویسندگان [English]

  • Alireza Shams
  • Atefeh Shamosi
Department of Anatomy, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
چکیده [English]

Background and Objective: Differentiation of bone marrow mesenchymal stem cells (BMMSC) has studied in different cell lines. Variety of biochemical and topographic signaling could influence and change cells differentiation and proliferation of a special cell line. The aim of this study was to evaluate potential differentiating of BMMSC to neuronal-like cells.
Materials and Methods: Freezed casting and freezed drying scaffolds of gelatin/polylactic-co-glycolic acid (PLGA) with iron nanoparticles (MNPs) and without iron nanoparticles (MNPs-free) was used as a three-dimensional topographic structure. BMMSC differentiation to neuronal-like cells by neurogenic culture was done in 20 days. BMMSC proliferation was studied by MTT assay. Gene expression of specific neuronal markers was studied by immunohistochemistry methods. In this study, one-way ANOVA, post hoc Tukey and Mann-Whitney nonparametric tests were used. 
Results: Gelatin PLGA- MNP freezed casting scaffold containing Fe nanoparticles provides a suitable structure for differentiation into neuron-like cells. The results of cell survival (Mean 0.7600 and standard deviation 0.02000 after 72 hours) and gene expression (Mean 79.00 and standard deviation 1.000) showed that the rate of cell proliferation on freezed casting scaffolds containing nanoparticles is higher than that of freezed drying scaffolds (P<0.001).
Conclusion: Freezed casting gelatin PLGA-MNP scaffold could be an appropriate choice for repair and regeneration of nervous system.

کلیدواژه‌ها [English]

  • Bone marrow mesenchymal stem cells
  • Differentiation
  • Freezed casting
  • Freezed drying

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