دانشور پزشکی

دانشور پزشکی

تأثیر هشت هفته تمرین تناوبی شدید و مصرف کافئین بر بیان گلیکوژن سنتاز و میزان گلیکوژن کبدی موش های بزرگ آزمایشگاهی دیابتی

نوع مقاله : مقاله پژوهشی

نویسندگان
معین نوروزی 1
عباس صادقی 2
محدثه فعال پاکدهی 2
قاسم ترابی 3
1 موسسه آموزش عالی علامه قزوینی، قزوین، ایران
2 گروه علوم ورزشی، دانشکده علوم اجتماعی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران
3 گروه فیزیولوژی ورزشی. دانشگاه آیت الله آملی، امل، ایران
10.22070/daneshmed.2021.14819.1101
چکیده
مقدمه و هدف: دیابت بیماری متابولیکی شایعی است که به اختلال در سنتز گلیکوژن کبدی منجرمی شود. پژوهش حاضر به تأثیر هشت هفته تمرین تناوبی  شدید (HIIT) و مصرف کافئین بر بیان گلیکوژن سنتاز (GYS2) و میزان گلیکوژن کبدی موش های بزرگ آزمایشگاهی دیابتی می‌پردازد.
مواد و روش ها: در یک مطالعه‌ی حیوانی بالینی- مداخله‌ای 50 سر موش بزرگ آزمایشگاهی ویستار که با استروپتوزوسین القا دیابت شدند به 5 گروه مساوی کنترل (C)، دیابتی (D)، دیابتی با مکمل (D+CAF)، دیابتی با تمرین (D+T)، دیابتی با مکمل و تمرین (D+CAF+T) تقسیم شدند. برنامه‌ی تمرین شامل هشت هفته، هفته‌ای 5 جلسه (6 تا 12 وهله 2 دقیقه‌ای با شدت 90-85 درصد سرعت ماگزیمم) بود و هفته‌ای پنج روز  mg/kg70 کافئین هیدراته تزریق شد. بعد از بیهوش کردن موش ها، بافت کبد استخراج و میزان بیان  GYS2و گلیکوژن کبدی ارزیابی شد. تحلیل داده‌ها با آزمون‌های t مستقل و تحلیل واریانس دو راهه در سطح معناداری (P<0.05) انجام شد.
نتایج: القای دیابت باعث کاهش معنی‌دار گلیکوژن کبدی و بیان GYS2 شد (۰۰1/۰>P). همچنین کافئین (۰0۱/۰>P) و HIIT (۰24/۰=P) هر دو موجب افزایش معنی‌دار GYS2 شدند که مصرف کافئین با اندازه اثر 44 درصدی تاثیر بیشتری داشت. همچنین HIIT (529/۰=P) و کافئین (761/۰=P) هیچ کدام بتنهایی  و در ترکیب با هم (12/۰=P) موجب افزایش معنی‌دار گلیکوژن کبدی نشدند.
نتیجه‌گیری: با توجه به نتایج تحقیق، احتمالاً بتوان تمرینات HIIT و مصرف کافئین را به‌عنوان مداخله‌ی مؤثر در بهبود بیان GYS2 پیشنهاد داد. هر چند اظهار نظر صریح تحقیقات بیشتری را در این زمینه می طلبد.
کلیدواژه‌ها
تمرین تناوبی شدید
کافئین
دیــابت
گلیکوژن
گلیکوژن سنتاز
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