تأثیر هشت هفته تمرین تناوبی شدید و مصرف کافئین بر بیان گلیکوژن سنتاز و میزان گلیکوژن کبدی موش های بزرگ آزمایشگاهی دیابتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 موسسه آموزش عالی علامه قزوینی، قزوین، ایران

2 گروه علوم ورزشی، دانشکده علوم اجتماعی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران

3 گروه فیزیولوژی ورزشی. دانشگاه آیت الله آملی، امل، ایران

چکیده

مقدمه و هدف: دیابت بیماری متابولیکی شایعی است که به اختلال در سنتز گلیکوژن کبدی منجرمی شود. پژوهش حاضر به تأثیر هشت هفته تمرین تناوبی  شدید (HIIT) و مصرف کافئین بر بیان گلیکوژن سنتاز (GYS2) و میزان گلیکوژن کبدی موش های بزرگ آزمایشگاهی دیابتی می‌پردازد.
مواد و روش ها: در یک مطالعه‌ی حیوانی بالینی- مداخله‌ای 50 سر موش بزرگ آزمایشگاهی ویستار که با استروپتوزوسین القا دیابت شدند به 5 گروه مساوی کنترل (C)، دیابتی (D)، دیابتی با مکمل (D+CAF)، دیابتی با تمرین (D+T)، دیابتی با مکمل و تمرین (D+CAF+T) تقسیم شدند. برنامه‌ی تمرین شامل هشت هفته، هفته‌ای 5 جلسه (6 تا 12 وهله 2 دقیقه‌ای با شدت 90-85 درصد سرعت ماگزیمم) بود و هفته‌ای پنج روز  mg/kg70 کافئین هیدراته تزریق شد. بعد از بیهوش کردن موش ها، بافت کبد استخراج و میزان بیان  GYS2و گلیکوژن کبدی ارزیابی شد. تحلیل داده‌ها با آزمون‌های t مستقل و تحلیل واریانس دو راهه در سطح معناداری (P<0.05) انجام شد.
نتایج: القای دیابت باعث کاهش معنی‌دار گلیکوژن کبدی و بیان GYS2 شد (۰۰1/۰>P). همچنین کافئین (۰0۱/۰>P) و HIIT (۰24/۰=P) هر دو موجب افزایش معنی‌دار GYS2 شدند که مصرف کافئین با اندازه اثر 44 درصدی تاثیر بیشتری داشت. همچنین HIIT (529/۰=P) و کافئین (761/۰=P) هیچ کدام بتنهایی  و در ترکیب با هم (12/۰=P) موجب افزایش معنی‌دار گلیکوژن کبدی نشدند.
نتیجه‌گیری: با توجه به نتایج تحقیق، احتمالاً بتوان تمرینات HIIT و مصرف کافئین را به‌عنوان مداخله‌ی مؤثر در بهبود بیان GYS2 پیشنهاد داد. هر چند اظهار نظر صریح تحقیقات بیشتری را در این زمینه می طلبد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of eight-week high intensity interval training (HIIT) and caffeine consumption on glycogen synthase expression and hepatic glycogen levels in diabetic rats

نویسندگان [English]

  • Moin Norozi 1
  • Abbas Sadeghi 2
  • Mohadesh Faal Pakdehi 2
  • Ghasem Torabi 3
1 Allameh Qazvini Institute of Higher Education, Qazvin, Iran
2 Department of Sports Sciences, Faculty of Social Sciences, Imam Khomeini International University, Qazvin, Iran
3 Department of Sports Sciences Ayatollah Amoli University, Amol, Iran
چکیده [English]

Background and Objective: Diabetes is a common metabolic disease that leads to impaired hepatic glycogen synthesis. The present study investigates the effect of eight weeks of intense intermittent exercise (HIIT) and caffeine consumption on glycogen synthase (GYS2) expression and liver glycogen levels in diabetic rats.
Materials and Methods: In an experimental clinical-intervention study, 50 streptozotocin-induced diabetic rats were divided into 5 equal groups of control (C), diabetic (D), supplemental diabetic (D + CAF), diabetic with exercise (D + T), supplement and exercise (D + CAF + T). The training program consisted of eight weeks, 5 sessions per week (6 to 12 2-minute sessions with an intensity of 85-90% of the maximum speed) and 70 mg/kg of caffeine were injected five days a week. After anesthesia, liver tissue was extracted and the expression levels of (GYS2) and liver glycogen were assessed. Data analysis was performed by independent t-test and two-way ANOVA at a significant level of (P<0.05).
Results: Induction of diabetes significantly reduced hepatic glycogen and GYS2 expression (P<0.001). Also, caffeine consumption (P<0.01) and HIIT (P=0.024) both significantly increased GYS2, which had a greater effect of caffeine with a 44% effect size. Also, HIIT (P=0.529) and caffeine (P=0.761) neither alone, nor in combination (P=0.12) caused a significant increase in hepatic glycogen.
Conclusion: According to results, it is possible to suggest HIIT and caffeine consumption as an effective intervention to improve (GYS2) expression. However, a clear statement requires further research in this area.

کلیدواژه‌ها [English]

  • High intensity interval training (HIIT)
  • Caffeine
  • Diabetes
  • Glycogen
  • Glycogen synthase

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