بررسی درصد و تعداد سلول های CD3- CD56+CD16+NK خون محیطی در زنان باردار دارای جنین IUGR

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه ایمونولوژی، دانشکده پزشکی شاهد، تهران، ایران

2 گروه پریناتولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی تهران، تهران، ایران

3 مرکز تحقیقات تنظیم پاسخ های ایمنی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: محدودیت رشد داخل رحمی (IUGR)، یکی از مشکلات بارداری است که تغییرات نامناسب عروق در این دوران، می تواند باعث بروز آن شود. با توجه به نقش سلول های NK در IUGR و مشکلات مطالعه آن ها در بافت، بررسی آن ها در خون محیطی ضروری است. هدف ما در این مطالعه بررسی درصد و تعداد سلول های NK+16CD+56CD-3  CD خون محیطی در زنان باردار دارای جنین IUGR است.
مواد و روش ها: از 18 زن باردار با جنین IUGR  و15 زن باردار با جنین سالم دعوت شد. تعداد و درصد سلول های NK+16CD+56CD-3  CD خون محیطی در دو گروه به روش فلوسایتومتری بررسی شد.
نتایج: درصد سلول‌های  NK+16CD+56CD-3 CDدر گروه بیمار و سالم، به ترتیب 11/24 و 17/26 مشاهده گردید. تعداد این سلول ها در گروه بیمار 27/97 و در گروه سالم 7/129 مشاهده گردید. با آنالیز آماری من- ویتنی تفاوت معنی داری بین دو گروه مشاهده نگردید. با آزمون اسپیرمن، ارتباط معنی داری بین سن و سلول های NK در گروه بیمار مشاهده شد.
نتیجه‌گیری: مقایسه درصد و تعداد سلول‌های NK+16CD+56CD-3 CD در گروه بیمار با گروه کنترل، تفاوت معنی داری نداشت که می تواند به دلیل عدم آنالیز زیرگروه های سلول NK باشد. در مطالعات بعدی آنالیز زیرگروه های NK بررسی گردد. با توجه به ارتباط معنی دار سن با تعداد و درصد سلول های NK در گروه بیمار، به نظر می رسد افزایش سن به عنوان یک فاکتور منفی در بارداری، با کاهش این سلول ها همراه است.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of peripheral blood CD3-CD56+CD16+NK cells percentage and count in pregnant women with IUGR fetus compared to pregnant women with normal fetus

نویسندگان [English]

  • Somayeh Hajmahmoodi 1
  • Sedigheh Hantoushzadeh 2
  • Tooba Ghazanfari 3
  • Maryam Rajabnia Chenary 1
1 Department of Immunology, School of Medicine, Tehran, Iran
2 Department of Perinatology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;
3 Immunoregulation Research Center, Shahed University, Tehran, Iran.
چکیده [English]

Background and Objective: Intrauterine growth restriction (IUGR) is one of the pregnancy problems that can be caused by improper vascular changes. Due to the role of NK cells in IUGR and the difficulties of studying them in tissues, it is necessary to study them in peripheral blood. The aim of this study was to evaluate the percentage and number of peripheral blood CD3-CD56+CD16+NK cells in pregnant women with IUGR fetus..
Materials and Methods: 18 pregnant women with IUGR fetus and 15 pregnant women with healthy fetus were invited. The number and percentage of peripheral blood CD3-CD56+CD16+NK cells in both groups were assessed by flow cytometry.
Results: The percentage of CD3-CD56+CD16+NK cells in the patient and healthy groups was 24.11 and 26.17, respectively. The number of these cells was 97.27 in the patient and 129.7 in the healthy group. No significant difference was observed between the two groups by Mann-Whitney analysis. Spearman analysis showed a significant relationship between age and NK cells in the patient group.
Conclusion: The comparison of percentage and number of CD3-CD56+CD16+NK cells in the patient group with the control group was not significantly different which could be due to the lack of analysis of NK cell subgroups. In future studies, analysis of NK subtypes should be investigated. Considering the significant relationship between age and the number and percentage of NK cells in the patient group, it seems that increasing age as a negative factor is associated with a decrease in these cells.

کلیدواژه‌ها [English]

  • Intrauterine growth restriction (IUGR)
  • NK cells
  • Peripheral blood

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دانشور پزشکی
دوره 29، شماره 3 - شماره پیاپی 154
مرداد و شهریور 1400
صفحه 57-65

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