اثرات عصاره آبی الکلی بخش‌های هوایی اسطوخودوس بر صرع لوب گیجگاهی ناشی از پیلوکارپین در موش بزرگ آزمایشگاهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی، دانشکده پزشکی دانشگاه شاهد، تهران، ایران

2 مرکز تحقیقات نوروفیزیولوژی دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: Lavandula dentata در ایران اسطوخودوس نامیده می شود. گزارش‌هایی مبنی بر اثرات ضد صرعی اسطوخودوس وجود دارد، ولی تا به حال گزارشی درباره اثر این گیاه بر صرع وجود ندارد. این مطالعه اثر Lavandula dentata بر تشنجات ناشی از پیلوکارپین را تحقیق کرده است.
مواد و روش ها: 75 سر موش بزرگ آزمایشگاهی نر با محدوده وزنی 250-200 گرم به پنج گروه 15 تایی تقسیم شدند که شامل:1- گروه کنترل سالین 2-گروه کنترل عصاره 3-گروه صرعی 4- گروه صرعی پیش درمان شده با تک دوز عصاره اسطوخودوس mg/Kg) 200) 5- گروه صرعی پیش درمان شده با دوزهای مکرر عصاره (mg/Kg 200). القای تشنجات در هر سه گروه اخیر با تزریق داخل صفاقی پیلوکارپین هیدروکلراید (mg/Kg 380) صورت گرفت. مالون دی الدئید (MDA) و گلوتاتیون مغز اندازه گیری شد. از نظر آماری از آزمون آنووای یکطرفه و یا آزمون کروسکال والیس و تست های تعقیبی مربوطه مثل توکی استفاده شد.
نتایج: تجویز لاوندولا دنتاتا در گروه پیش درمان موجب تأخیر در بروز Status Epilepticus (SE)، کاهش طول مدت SE و کاهش میزان مرگ و میر نسبت به گروه صرعی شد. پیش درمان با عصاره میزان گلوتاتیون مغز را 24 ساعت پس از تجویز پیلوکارپین، به طور معناداری افزایش داد ولی اثر مهاری بر میزان MDA نداشت.
 نتیجه گیری: لاوندولا دنتاتا شدت تشنجات و مرگ و میر ناشی از  SEالقاشده توسط تجویز پیلوکارپین را به طور معنی داری کاهش داد. به نظر می رسد افزایش فعالیت آنتی اکسیدانی مانند افزایش گلوتاتیون مغز تا حدودی مسئول چنین نتایجی باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The effects of aerial parts hydroalcoholic extract of Lavandula dentata in the pilocarpine rat model of temporal lobe epilepsy

نویسندگان [English]

  • Shekoofe Azimi 1
  • Batool Rahmati 1
  • Mehdad Roghani 2
  • Ladan Sedighnejad 1
1 Department of Physiology, School of Medicine, Shahed University, Tehran, Iran
2 Neurophysiology Research Center, Shahed University, Tehran, Iran
چکیده [English]

Background and Objective: Lavandula dentata is called Ustukhuddus in Iran .There are some reports about the anticonvulsant effects of Ustukhuddus but, the effect of this plant on epilepsy is not reported yet. In this study the effect of Lavandula dentata on seizures induced by pilocarpine was investigated.
Materials and Methods: A total of 75 male rats weighing 200-250 grams were divided into five groups of 15, which included: 1- control saline 2- control extract 3- Epileptic group 4- Epileptic group treated with single doses of lavender extract 200 mg / Kg  5- epileptic group treated with repeated dose of lavender extract (200 mg / Kg). The induction of seizures in all three groups was done by intra-peritoneal injection of pilocarpine hydrochloride (380 mg / kg).  Brain malondialdehyde (MDA) and glutathione was measured. Statistical analysis was carried out using one way ANOVA or Kruskal–Wallis oneway analysis of variance by ranks followed by post-hoc tukey test.
Results: The administration of Lavandulla dentata in the pretreatment groups delayed the onset of status epilepticus (SE), reduced the duration of SE and decreased the mortality rate compared to the epileptic group. Pretreated extract increased significantly brain glutathione levels 24 hours after pilocarpine administration, but did not exhibit inhibitory effect on brain MDA.
Conclusion: Lavendula dentata reduced significantly seizure intensity and the mortality rate due to SE induced by pilocarpine administration. It seems that antioxidant activity such as brain glutathione enhancement was partly responsible for such results.  

کلیدواژه‌ها [English]

  • Lavender
  • Pilocarpine
  • Temporal lobe epilepsy
  • Rat
  • Oxidative stress

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دانشور پزشکی
دوره 29، شماره 1 - شماره پیاپی 152
فروردین و اردیبهشت 1400
صفحه 35-48

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