بررسی فراوانی اینتگرون 1 و 2 و ارتباط آنها با الگوی مقاومت دارویی در بیماران مبتلا به گاستروانتریت در سویه های سالمونلا در تهران، ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه میکروب‌شناسی، دانشکده پزشکی، دانشگاه شاهد تهران. ایران

2 دانشگاه آزاد اسلامی، واحد علوم تحقیقات، گروه زیست‌شناسی، تهران، ایران

3 مرکز تحقیقات عفونی اطفال، پژوهشکده سلامت کودکان، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران

چکیده

مقدمه و هدف: سالمونلا یکی از باکتری های مهم ایجادکننده گاستروانتریت در انسان بوده و گسترش سویه های سالمونلا با مقاومت چند دارویی یک مشکل حاد جهانی است. اینتگرون ها از مهمترین عوامل مسئول انتقال ژنهای مقاومت به دارو در بین سروتیپ های سالمونلا هستند. در مطالعه حاضر، فراوانی اینتگرون های کلاس 1 و کلاس 2 و همچنین ارتباط آنها با الگوهای مقاومت دارویی بیماران با علائم گوارشی بررسی شد.
مواد و روش ها: در این مطالعه مقطعی، 400 نمونه مدفوع انسانی با علائم گوارشی از 4 بیمارستان در شهر تهران تهیه شد. تمامی نمونه ها توسط روش استاندارد از نظر کشت میکروبی بررسی و تائید نتایج توسط آزمایش های بیوشیمیایی و PCR انجام گرفت. آنتی بیوگرام توسط روش انتشار از دیسک صورت پذیرفت. پس از استخراج DNA حضور اینتگرون های کلاس 1 و 2 با PCR بررسی گردید. آنالیز آماری جهت سنجش ارتباط بین الگوهای مقاومت دارویی و حضور اینتگرون ها توسط نرم افزار SPSS و آزمون فیشر انجام گرفت.
نتایج: نتایج نشان داد که سالمونلا در 5.5٪ (400/22) از نمونه های مدفوع با علامت گوارشی وجود دارد. میزان شیوع اینتگرون کلاس 1 و 2 برای نمونه های مدفوع انسانی به ترتیب 9/40% (22/9) و 2/27% (22/6) بود. فنوتیپ MDR در 4.5٪ (1.22) جدایه های سالمونلا از نمونه های مدفوع انسانی شناسایی شد.
نتیجه‌گیری: در این مطالعه میزان بالایی از حضور اینتگرون های 1 و 2 در جدایه های مدفوع بیماران با علائم گاستروانتریت مشاهده شد. ارتباط مشاهده شده میان حضور اینتگرون ها و الگوهای مقاومت دارویی نشان دهنده احتمال افزایش ریسک انتقال ژنهای مقاومت دارویی در سویه های سالمونلای مسئول عفونت انسانی است.

کلیدواژه‌ها

عنوان مقاله [English]

Frequency of integrons 1 and 2 and their relationship with drug resistance patterns of Salmonella isolates in patients with gastroenteritis in Tehran, Iran

نویسندگان [English]

  • Saeid Besharati 1
  • Parviz Owlia 1
  • Atena Sadeghi 1
  • Fateme Ahmadi 2
  • Masoud Alebouyeh 3
1 Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
2 Department of Microbiology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Pediatric Infections Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده [English]

Background and Objective: Salmonella is one of the most important bacteria that causes gastroenteritis in humans and the spread of Salmonella strains with multidrug resistance is an acute global problem. Integrons are one of the most important factors responsible for transfering of drug resistance genes among Salmonella serotypes. In the present study, the frequency of class 1 and class 2 integrons as well as their relationship with drug resistance patterns in patients with gastrointestinal symptoms were investigated.
Materials and Methods: In this cross-sectional study, 400 human fecal samples with gastrointestinal symptoms were collected from 4 hospitals in Tehran. All the samples were analyzed by standard method for microbial culture and the results were confirmed by biochemical and PCR tests. Antimicrobial susceptibility testing was performed by disk diffusion method. After DNA extraction, the presence of class 1 and 2 integrons was investigated by PCR. Statistical analysis for detection of correlation between the presence of integrons and resistance patterns was done using SPSS software and Fisherʼs exact test.
Results: The results showed that Salmonella was present in 5.5% (22.400) of the stool specimens. The prevalence rates of class 1 and 2 integrons for human stool specimens were 40.9% (9/22) and 27.2% (6/22), respectively. MDR phenotype was detected in 4.5% (1.22) of the Salmonella isolates from patients with gastroenteritis.
Conclusion: In this study, high levels of integrons 1 and 2 were observed in the patients' Salmonella isolates with gastroenteritis. The observed relationship between drug resistance patterns and the integrons indicated a possible increased risk of drug resistance genes in Salmonella isolates with human gastroenteritis.

کلیدواژه‌ها [English]

  • Salmonella
  • Integrons
  • Drug Resistance
  • Gastroenteritis

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