بررسی شیوع ژنهای MepA, MDeA, NorA و NorC در سویه های استافیلوکوکوس اورئوس جداسازی شده از بیمارستانهای شهر تهران

نویسندگان

1 گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه بیولوژی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

مقدمه و هدف: امروزه برخی از سویه‌های استافیلوکوک که به سویه‌های استافیلوکوکی مقاوم به متیسیلین و یا به اختصار MRSA معروف هستند، عفونت‌هایی را ایجاد می‌کنند که به روش‌های درمانی و آنتی‌بیوتیک‌های مرسوم مقاوم هستند. یکی از مهمترین دلایل این مقاومت وجود پمپهای افلاکس مقاومت دارویی در این سویه‌ها است. هدف از انجام این پروژه بررسی میزان شیوع ژنهای پمپ افلاکس NorA، NorC، MdeA و MepA در سویه‌های بالینی استافیلوکوکوس اورئوس بود.
مواد و روش ها: 250 نمونه بالینی از بیمارستانهای شهر تهران جداسازی گردید و با استفاده از روش‌های میکروب‌شناسی سویه‌های استافیلوکوک اورئوس شناسایی شدند. نتایج جداسازی میکروبی نشان داد که 50 نمونه (20%) از نمونه‌های جداسازی شده متعلق به ایزوله های استافیلوکوکوس اورئوس است. سپس با استفاده از روش‌های دیسک دفیوژن میزان مقاومت سویه‌های استافیلوکوکوس اورئوس به آنتی‌بیوتیک‌های مختلف بررسی شد. استخراج DNA از سویه‌ها صورت گرفت و واکنش PCR جهت بررسی وجود ژنهای NorA، NorC، MdeA و MepA انجام گردید.
نتایج: 68 درصد سویه‌ها مقاوم به متی‌سیلین (MRSA) و 56 درصد از سویه‌های MRSA به سیپروفلوکساسین هم مقاوم هستند. همچنین نتایج PCR نشان داد که میزان شیوع ژنهای NorA، NorC، MdeA و MepA در سویه‌های مقاوم به سیپروفلوکساسین استافیلوکوکوس اورئوس به ترتیب 60 درصد، 16 درصد، 6 درصد و 10 درصد است.
نتیجه‌گیری: با توجه به شیوع ژنهای پمپ افلاکس در سویه های مقاوم به سیپروفلوکساسین و متیسیلین، می‌باید از راهکارهایی بهتر در مورد پیشگیری و درمان عفونت‌های مرتبط با سویه‌های مقاوم به متیسیلین استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of prevalence of MepA, MdeA, NorA and NorC genes in Staphylococcus aureus strains isolated from Tehran hospitals

نویسندگان [English]

  • Maryam Shakiba 1
  • Fatemeh Ashrafi 2
چکیده [English]

Background and Objective: Today some Staphylococcus strains are known as methicillin-resistant Staphylococcus aureus or MRSA causes infections which are resistant to conventional therapies and antibiotics. One of the most important reasons for this resistance is the presence of efflux pumps in these strains. The purpose of this project was to investigate the prevalence of NorA, NorC, MdeA and MepA germs in clinical strains of Staphylococcus aureus.
Materials and Methods: 250 clinical samples were isolated from Tehran hospitals and identified by Staphylococcus aureus strains using microbiological methods. Results of microbial isolation showed that 50 samples (20%) out of them belong to Staphylococcus aureus isolates. Continuously, the resistance of Staphylococcus aureus strains to different antibiotics was investigated by using diffusion disk method. DNA extraction of strains was performed and in order to investigate presence of NorA, NorC, MdeA and MpeA genes by using PCR method.
Results: 68% of strains were methicillin resistant (MRSA) and 56% of MRSA strains were also resistant to ciprofloxacin. Also, the results of PCR assay demonstrated that the prevalence level of NorA, NorC, MdeA and MpeA genes in the staphylococcus resistance to ciprofloxacin were 60%, 16%, 6% and 10%, respectively.
Conclusion: According to the prevalence of the efflux pump genes in resistant strains to ciprofloxacin and methicillin, better approaches should be utilized to prevent and treat the infections associated with methicillin-resistant strains.

کلیدواژه‌ها [English]

  • Staphylococcus aureus
  • NorA
  • NorC
  • MdeA
  • MepA
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