تتعیین فراوانی، مقاومت آنتی‌بیوتیکی و سرگروه‌های سالمونلا در میان نمونه‌های گوشت مرغ در شهر تهران، ایران

نویسندگان

1 گروه میکروب‌شناسی، دانشکده پزشکی، دانشگاه شاهد تهران. ایران

2 مرکز تحقیقات میکروب‌شناسی مولکولی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

3 گروه زیست‌شناسی، واحد علوم تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

4 مرکز تحقیقات میکروب‌شناسی بالینی استاد البرزی، دانشگاه علوم پزشکی شیراز، شیراز، ایران

5 مرکز تحقیقات عفونی اطفال، پژوهشکده سلامت کودکان، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران

چکیده

مقدمه و هدف: سالمونلا یکی از عوامل اصلی بیماری منتقله از طریق غذا (Foodborne) در سطح جهانی بوده که به دلیل افزایش روزافزون مقاومت دارویی در آنها امروزه به یک مسئله مهم تبدیل شده است. هدف از این مطالعه تعیین فراوانی و تنوع گونه‌های سالمونلا از نظر مقاومت دارویی و تنوع سرگروهی آنها در نمونه‌های گوشت مرغ شهر تهران بود.
 
مواد و روش­ها: در این مطالعه مقطعی، 100 نمونه گوشت مرغ خریداری شده از واحدهای مجاز توزیع کننده محصولات گوشتی از 22 منطقه تهران تهیه شد. تمامی نمونه­ها توسط روش استاندارد از نظر کشت میکروبی بررسی و تأیید نتایج توسط آزمایش‌های بیوشیمیایی و سروگروپینگ انجام گرفت. آنتی بیوگرام توسط روش انتشار از دیسک صورت پذیرفت.
 
نتایج: نتایج نشان داد که سالمونلا در 75٪ (75/100) از نمونه ­های گوشت مرغ وجود دارند. جدایه­های گوشت مرغ عمدتاً متعلق به سرگروه C بود (88٪) (75/66)، در حالی که سایر جدایه های مربوط به سرگروه B 6/2٪ (75/2)، سرگروه  D3/5٪ (75/4) و غیر گروهA - D3/5٪ (75/4) بودند. مقاومت همزمان به 3 کلاس آنتی‌بیوتیک‌ها (3DR) شایع‌ترین نوع مقاومت چند دارویی (MDR) در بین جدایه های مرغ بود. بیشترین مقاومت آنتی‌بیوتیکی به تتراسایکلین مشاهده شد (%59).
 
نتیجه­ گیری: در این مطالعه میزان بالایی از آلودگی به سالمونلا در نمونه‌های گوشت مرغ مشاهده شد. الگوهای مقاومت دارویی شایع در این جدایه ها نشان‌دهنده ریسک احتمالی این سویه‌ها در انتقال ژنهای مقاومت دارویی طی زنجیره غذایی به اعضای میکروبیوتای دستگاه گوارش انسان باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Frequency, antibiotic resistance, and serogroups of Salmonella among chicken meat specimens in Tehran, Iran

نویسندگان [English]

  • Saeid Besharati 1
  • Parviz Owlia 2
  • Atena Sadeghi 1
  • Fatemeh Ahmadi 3
  • Fereshteh Fani 4
  • Gholamreza Puladfar 4
  • Masoud Alebouyeh 5
چکیده [English]

Background and Objective: Salmonella is one of the leading causes of foodborne illnesses worldwide which has become an important issue today due to the increasing drug resistance. This study was aimed to detect the frequency and diversity of Salmonella serogroups and drug resistance patterns among poultry meat samples distributed in Tehran, Iran.
 
Materials and Methods: In this cross-sectional study, 100 samples of poultry meat were prepared from authorized distributors of meat products from 22 districts of Tehran. All samples were analyzed by standard method and characterization of the isolates were done using biochemical, polymerase chain reaction, and serogrouping methods. Antibiogram was done using disk diffusion method.
 
Results: The results showed that Salmonella was present in 75% (75/100) of the chicken meat samples. Chicken meat isolates were predominantly belonged to serogroup C (88%, 66/75), while other isolates belonged to serogroup B (2.6%, 2/75), serogroup D (5.3%, 4/75), and non-group A–D Salmonella isolates (5.3%, 4.75). While resistance to tetracycline (59%) was the most common resistance phenotype in these isolates, concurrent resistance to 3 classes of antibiotics (3DR) was the most common type of multidrug resistance (MDR) phenotype among them.
 
Conclusion: In this study, high rate of contamination with Salmonella was observed in the chicken meat samples. Dominance of antibiotic resistance in these isolates showed their possible risk for transmission of resistance gene markers to the human gut microbiota through food chain.
 

کلیدواژه‌ها [English]

  • Salmonella
  • Serogroups
  • Chicken meat
  • Antimicrobial resistance
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