اثر نوبیلتین بر آپوپتوز در مغز میانی ونئواستریاتوم در مدل تجربی بیماری پارکینسون القاشده با تزریق داخل نیگرال لیپوپلی ساکارید در موش صحرایی

نویسندگان

1 گروه فیزیولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

2 مرکز تحقیقات نوروفیزیولوژی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: نوبیلتین ، فلانوئید موجود در مرکبات است. خاصیت آنتی‌اکسیدانی و ضدالتهابی و ضد آپوپتوزی نوبیلتین در بسیاری از مطالعات اخیر مشاهده شده است. هدف مطالعه حاضر، بررسی اثر نوبیلتین بر شدت آپوپتوز در مدل تجربی بیماری پارکینسون ایجاد شده با تزریق داخل نیگرال لیپوپلی ساکارید در موش صحرایی بود.
 
مواد و روش‌ها: موش‌های صحرایی نر (28n =) به چهار گروه شم، شم تحت تیمار با نوبیلتین، ضایعه‌ دیده و ضایعه ‌دیده تحت درمان با نوبیلتین تقسیم ‌شدند. با تزریق 5 میکروگرم لیپوپلی‌ساکارید داخل سمت راست ناحیه جسم سیاه موش‌های صحرایی در سیستم نیگرااستریاتال ضایعه ایجاد شد. نوبیلتین به میزان 10 میلی‌گرم بر کیلوگرم از یک ساعت بعد از جراحی تا یک هفته بعد آن به طور روزانه و به شکل خوراکی تجویز شد. در پایان هفته اول پس از جراحی هموژنه بافتی تهیه شده و اندازه‌گیری شاخص شدت آپوپتوز انجام شد.
 
یافته‌ها: نتایج نشان داد که درمان با نوبیلتین به کاهش معنی‌دار میزان DNA fragmentation در گروه LPS تیمارشده با نوبیلیین در مقایسه با گروه LPS در مغز میانی منجر ‌شد (P<0.05).
 
نتیجه‌گیری: تجویز نوبیلتین به موش‌های پارکینسونی شده با لیپوپلی‌ساکارید موجب کاهش آپوپتوز می‌شود و احتمالاً می‌تواند در درمان کمکی بیماری پارکینسون تأثیر داشته باشد.
 

کلیدواژه‌ها


عنوان مقاله [English]

The effect of nobiletin on apoptosis in midbrain and neostriatum in a rat model of Parkinson’s disease induced by intranigral injection of lipopolysaccharide

نویسندگان [English]

  • Sedigheh Keshtkar 1
  • Zahra Kiasalari 2
  • Maryam Khorasani 1
  • Marzieh Fakour 1
  • Reihane Ghasemi 1
  • Mehrdad Roghani 2
چکیده [English]

Background and Objective: Nobiletin is a flavonoid in the citrus. The antioxidant, anti-inflammatory and anti-apoptotic properties of nobiletin have been observed in many recent studies. The aim of the present study was to investigate the effect of nobiletin on apoptosis in an experimental model of Parkinson's disease induced by intranigral injection of lipopolysaccharide in the rat.
 
Materials and Methods: Male rats (n = 28) were divided into four groups: sham, sham under treatment with nobiletin, lesion, and lesion treated with nobiletin. To achieve unilateral lesion of the nigrostriatal system, rats received 5 microgram of LPS into the right substantia nigra. Nobiletin was daily administered p.o. at a dose of 10 mg/kg one hour after surgery for one week. At the end of the first week, tissue homogenate was prepared and severity of apoptosis was measured.
 
Results: The results showed that treatment with nobiletin decreases the level of DNA fragmentation in the LPS group as compared to the LPS group in midbrain area (p <0.05).
 
Conclusion: Administration of nobiletin to lipopolysaccharide-induced Parkinsonian rats reduces apoptosis and may be potentially effective for ancillary therapy of Parkinson's disease.
 

کلیدواژه‌ها [English]

  • Nobiletin
  • Apoptosis
  • Parkinson's disease
  • Lipopolysaccharide
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