تأثیر یک دوره تمرین تناوبی خیلی شدید بر کلاژن – 1 و TGF-β1 و عملکرد قلبی عضله قلب موش صحرایی پس از ایسکمی- ریپرفیوژن

نویسندگان

1 گروه فیزیولوژی ورزش دانشگاه تهران

2 گروه فیزیولوژی پزشکی دانشگاه علوم پزشکی ایران

چکیده

هدف: تأثیر یک دوره HIIT بر کلاژن – 1 و TGF-β1 و عملکرد قلبی عضله قلب موش صحرایی پس از ایسکمی- ریپرفیوژن است.
 
مواد و روش‌ها: 32 سر موش با تزریق تیوپنتال سدیم بی‌هوش شدند. انتوبه کردن، اتصال به ونتیلاتور و با بستن شریان کرونری نزولی بعد از توراکتومی، ایسکمی و 30 دقیقه بعد، ریپرفیوژن انجام شد. به صورت تصادفی به 4 گروه کنترل/ایسکمی، تمرین/ایسکمی، تمرین و شم تقسیم شدند.(گروه تمرین بدون جراحی و شم بدون ایسکمی). پس از 4 هفته دوره بازیافت، برنامه تمرینی (8 هفته، هفته‌ای 3 روز) 40 دقیقه­ای از 5 دقیقه گرم کردن و سرد کردن و 30 دقیقه دویدن تناوبی با شدت 85% تا 90% VO2max بود که با دوره فعال استراحت با شدت 50% تا 60% VO2max جدا می­شد. 48 ساعت پس از آخرین جلسه تمرینی و بی‌هوشی موش­ها، بافت قلب جدا شد و بیان ژن کلاژن-1 و TGF-β1 به روش PCR-RT سنجیده شد.
 
نتایج: بیان ژن کلاژن – 1 در گروه تمرین/ایسکمی در مقایسه با گروه کنترل/ ایسکمی کاهش معناداری داشت (05/0P <). میران بیان TGF-β1 در سه گروه در مقایسه با گروه کنترل/ایسکمی کاهش معناداری داشت (05/0P <). کسر تزریقی (021/0P = ) و کسر کوتاه شدگی ( 011/0P =) در گروه تمرین/ایسکمی در مقایسه با گروه ایسکمی افزایش معناداری را نشان داد.
 
نتیجه‌گیری: اجرای HIIT باعث کاهش کلاژن-1 و TGF-β1 و افزایش بهبود عملکرد قلبی بعد از ایسکمی شد.
 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of one period of high-intensity interval training on myocardial collagen-1 and TGF-β1 and cardiac function in post ischemia-reperfusion rats

نویسندگان [English]

  • Reza Golbashi 1
  • Abbasali Gaeini 1
  • Mohammad Reza Kordi 1
  • Nahid Aboutaleb 2
  • Alireza Ghardashi Afousi 1
چکیده [English]

Objective: This study was conducted to evaluate the effect of a period of high-intensity interval training (HIIT) on myocardial collagen-1 and TGF-β1 and cardiac function in post ischemia-reperfusion rats.
Materials and Methods: Thirty two (32) rats were anaesthetized by thiopental sodium. Intubation and connecting to ventilator and LAD ligation after thoracotomy was conducted. The ischemia was by 30 minutes and reperfusion was performed after ischemia. Rats were randomly divided into four groups: control-ischemia, Ischemia-HIIT, HIIT, and sham (HIIT groups without surgery, Sham groups without training). After 4 weeks of recovery, exercise training was performed (8 weeks, 3 days per week) 40 min including 5 min warm- up and cool- down and 30 min interval running at 85-90 % VO2max and separated by interval recovery at 50-60 % VO2max. 48 hours after last training session, rats were anesthetized and heart was removed. The gene expression of collagen-I and TGF-β1 was measured with PCR-RT method.
Results: Data analyzed showed that collagen 1 expression was significantly lower in Ischemia-HIIT group compared with control-ischemia group (p<0.05). Expression of TGF-β1 was significantly lower in three groups as compared to control-ischemia group (p<0.05). In addition, EF (p=0.021) and FS (P=0.011) was significantly greater in Ischemia–HIIT group as compared to Control-ischemia.
Conclusion: HIIT is an efficiency factor for decreasing TGF-β1 and collagen-1 and increasing cardiac function.

کلیدواژه‌ها [English]

  • High intensity interval training
  • Collagen-1
  • Transforming growth factor beta1
  • Myocardial ischemia
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