تغییرات بیان ژن فاکتور رشد شبه انسولینی 1 ((IGF-I در عضله دوقلوی موش‌های نر صحرایی نژاد ویستار پس از یک دوره تمرین تناوبی خیلی شدید

نویسندگان

گروه فیزیولوژی ورزشی، دانشکده تربیت‌بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

چکیده

مقدمه و هدف: پژوهش­ها نشان می­دهند فاکتور رشد شبه انسولینی 1 (IGF-I)، عامل اصلی رشد و هیپرتروفی تار­های عضلانی است. با وجود این، تأثیر تمرین­های تناوبی خیلی شدید (HIIT) بر IGF-I به خوبی بررسی نشده است. هدف از پژوهش حاضر بررسی تغییرات بیان ژن IGF-I در عضله دوقلوی موش­های نر صحرایی نژاد ویستار پس از یک دوره تمرین تناوبی خیلی شدید بود.
 
مواد و روش­ها: در این مطالعه 16 سر موش صحرایی نر نژاد ویستار با محدوده وزنی 25 ± 225 گرم تصادفی به دو گروه کنترل (n=8) و تمرین (n=8) تقسیم شدند. پس از یک هفته آشنایی با پروتکل تمرین، برنامه تمرین HIIT به مدت 40 دقیقه، سه روز در هفته و به مدت هشت هفته اجرا شد. 48 ساعت پس از آخرین جلسه تمرینی، عضله دو­قلو استخراج و میزان بیان ژن IGF-I به روش Real time-PCR سنجیده شد. برای تجزیه و تحلیل داده­های آماری از آزمون t مستقل استفاده شد.
 
نتایج: تحلیل آماری نشان داد بیان IGF-I عضله دوقلو در گروه تجربی در مقایسه با گروه کنترل بیش از 6 برابر (638 درصد) افزایش داشت (P = 0.016). وزن عضله دوقلو در گروه تمرینی در مقایسه با گروه کنترل افزایش معناداری داشت (P = 0.012).
 
نتیجه­ گیری: تمرین­ ورزشی HIIT به افزایش بارز بیان ژن IGF-I و وزن عضله دوقلوی موش­های صحرایی منجر شد.
 

کلیدواژه‌ها


عنوان مقاله [English]

Changes of insulin-like growth factor I gene expression in gastrocnemius muscle of male Wistar rats after a period of high-intensity interval training

نویسندگان [English]

  • Hanieh Nasrollahi
  • Abbas Ali Gaeini
  • Soheil Biglari
  • Alireza Ghardashi Afousi
چکیده [English]

Background and Objective: Studies have shown that insulin-like growth factor (IGF-I) is the main factor of growth and hypertrophy of muscle fibers. However, the effect of high-intensity interval training (HIIT) on IGF-I has not been well studied. The purpose of the present study was to investigate the changes of IGF-I gene expression in gastrocnemius muscle of male Wistar rats after a period of high-intensity interval training.
 
Materials and Methods: In this research study, 16 male Wistar rats (mean weight: 225±25 g) were randomly assigned into two groups: training (n=8) and control (n=8). After a week of familiarizing with training protocol, HIIT program was done 40 minutes each session, three sessions in a week for eight weeks. Then, 48 hours after the last training session, gastrocnemius muscle was extracted and the expression of IGF-I gene was determined by Real time-PCR technique. For statistical data analysis, independent t-test was used.
 
Results: Statistical analysis showed that the value of gastrocnemius muscle IGF-I in the experimental group compared with the control group increased by more than 6 times (638%) (P=0.016). Gastrocnemius muscle weight was significantly increased in the exercise group compared with the control group (P=0.012).
 
Conclusion: HIIT exercise led to a marked increase in rats` IGF-I gene expression and gastrocnemius muscle weight.
 

کلیدواژه‌ها [English]

  • HIIT
  • Hypertrophy
  • Gene expression
  • Real time-PCR
  • IGF-I
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