نویسندگان
1 دانشگاه ملایر –دانشکده علوم ،گروه زیست شناسی ،ملایر ،ایران
2 پژوهشکده نانوبیوتکنولوژی پژوهشگاه فناوری های نوین علوم زیستی جهاد دانشگاهی- ابن سینا،تهران،ایران
3 دانشگاه خوارزمی تهران، دانشکده علوم زیستی، گروه علوم جانوری، تهران، ایران
4 گروه پژوهشی مهندسی بافت پژوهشگاه فناوری های نوین علوم زیستی جهاد دانشگاهی- ابن سینا ،تهران،ایران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
Background and Objective: In recent decades, stem cell therapy has been introduced as a novel therapeutic approach for patients suffering from cardiac disorders. Recently, identification of menstrual blood-derived stem cells (MenSCs) as a unique source of stem cell with some characteristics as well as ease of access, high proliferative ability and renewability has created enormous promise for cell therapy.
Materials and Methods: In this study, differentiation ability of MenSCs into cardiomyocytes has been investigated. After MenSCs immunophenotyping, their differentiation into cardiomyocyte was investigated in the presence of 5-azacytidine and basic-fibroblast growth factor. Then, expression of the putative myogenic cells at mRNA and protein levels was determined by immunofluorescent staining and real-time quantitative PCR.
Results: Based on flow cytometric analysis, the isolated MenSCs typically expressed mesenchymal stem cell markers like CD105, CD73, CD44 and CD166 in parallel to OCT-4 as an embryonic marker. The differentiated MenSCs expressed cardiomyocyte markers at mRNA/protein level. The myogenic cells differentiated from MenSCs were positive for Connexin-43 and troponin T2 (TNNT2) protein. The mRNAs of Connexin-43, Connexin-40, Alpha actinin, Tropomyosin1 and TNNT2 were highly expressed in the differentiated myogenic cells.
Conclusion: Based on our data, MenSCs are a unique cell population with differentiation ability into cells with characteristics commonly attributed to cardiomyocytes.
کلیدواژه‌ها [English]
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