نویسندگان
1 گروه ژنتیک، دانشکده پزشکی، دانشگاه علوم پزشکی و خدمات درمانی شهید بهشتی
2 باشگاه پژوهشگران جوان، دانشگاه آزاد اسلامی، واحد تهران شرق
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Background and Objective: Helicobacter
pylori (H. pylori) infection is one of
the most common gastrointestinal infections worldwide. Infection with H. pylori strains may results in different pathological
manifestation and increased oxidative stress lead to a strong inflammatory
response in gastric mucosa. There is continuing interest in identifying H. pylori
virulence factors that might predict the risk for symptomatic clinical
outcomes. The prevalence of cagA and vacA genes, proteins and were
determined.
Materials and Methods: The presence of IgG antibody against CagA
and VacA proteins was determined by using Western blotting technique. The presence of cagA gene and vacA alleles was examined by PCR. H. pylori-positive patients including PUD and NUD were used for these experiments. Results: Biopsies
were considered as H. pylori-positive and negative when both the rapid
urease test and bacterial culture gave positive and negative results
respectively. H. pylori-positive, cagA-positive, and vacA alleles (s1 and m2)
were predominant in all clinical outcomes. There was no significant association
between prevalence of CagA and VacA status and clinical outcomes. Conclusion: Our results suggest
that cagA-positive strains were predominant in patients. However, we
found no association between cagA and vacA status
and clinical outcomes and this virulence
factor is not associated with the development of PUD. In addition,
serological tests such as the western blotting are helpful in detecting
subjects infected with H. pylori strains in PUD and NUD.
کلیدواژهها [English]
)