اثر محافظتی گرلین بر تغییرات هیستوپاتولوژیکی بافت کلیه موش های تحت تیمار با سیکلوفسفامید

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کمیته تحقیقات دانشجویی، دانشکده پیراپزشکی، دانشگاه علوم پزشکی اردبیل، اردبیل، ایران

2 کمیته تحقیقات دانشجویی، دانشکده پزشکی، دانشگاه علوم پزشکی اردبیل، اردبیل، ایران

3 گروه فیزیولوژی ورزشی، دانشگاه ارومیه، ارومیه، ایران

4 گروه علوم تشریح، دانشکده علوم پزشکی، دانشگاه علوم پزشکی مراغه، مراغه، ایران

5 گروه علوم تشریحی و پاتولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی اردبیل، اردبیل، ایران

10.22070/daneshmed.2021.13831.1035

کلیدواژه‌ها


عنوان مقاله [English]

Protective effect of ghrelin on histopathological alterations in renal tissue of mice treated with cyclophosphamide

نویسندگان [English]

  • Haniyeh AbdolahZadeh 1
  • Fatemeh Khoshdel 2
  • Fatemeh Ashory 3
  • Parviz Vahedi 4
  • Ramin Salimnejad 5
1 Students Research Committee, School of Paramedical Sciences, Ardabil University of Medical Sciences, Ardabil, Iran.
2 Students Research Committee, School of Medical Sciences, Ardabil University of Medical Sciences, Ardabil, Iran.
3 Department of Sports Physiology, Urmia University, Urmia, Iran.
4 Department of Anatomical Sciences, MaraghehUniversity of Medical Sciences Maragheh, Maragheh, Iran.
5 5. Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
چکیده [English]

Background and Objective: Cyclophosphamide is one of the common medications in chemotherapy and suppression of the immune system in organ transplantation. The clinical use of cyclophosphamide has been reduced due to its many side effects due to reproductive, hepatic, renal, and cardiac toxicity in patients and animal models. This study aimed to evaluate the effect of ghrelin against cyclophosphamide-induced damage to renal tissue.
Materials and Methods: Thirty-two (2-month-old) male mice were randomly divided into 4 groups: 1) control 2) cyclophosphamide (3) cyclophosphamide + ghrelin 4) ghrelin. Cyclophosphamide once a week (100 mg/kg) and ghrelin (80 μg/kg) were injected intraperitoneally daily for 5 weeks. After 5 weeks, the kidneys were removed and after weighing, tissue alterations were examined by hematoxylin-eosin and Schiff periodic acid staining.
Results: Studies showed that cyclophosphamide significantly reduced body weight and kidney weight and also significantly increased tissue damage (p < 0.05). Ghrelin treatment significantly improved the above parameters (p < 0.05).
Conclusion: The results of this study showed that ghrelin can reduce the destructive effects of cyclophosphamide on renal tissue.

کلیدواژه‌ها [English]

  • Cyclophosphamide
  • Ghrelin
  • Kidney
  • mice