اثر دو نوع تمرین (تداومی و تناوبی) و داروی آترواستاتین بر بیان ژن‌های VEGF و MMP2 بافت آئورت موش‌های سفید بزرگ آزمایشگاهی مدل دیابتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران

2 گروه علوم و فناوری های زیستی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران

چکیده

مقدمه و هدف: نشان داده شده است که حدود 80 درصد مرگ و میر در بیماران دیابتی ناشی از اختلالات قلبی عروقی است که به آن بیماری قلبی دیابتی گفته می‌شود، هدف از پژوهش حاضر بررسی اثر دو نوع تمرین (تداومی و تناوبی) و داروی آترواستاتین بر بیان ژن‌های VEGF و MMP2 بافت آئورت موش‌های سفید بزرگ آزمایشگاهی مدل دیابتی بود.
مواد و روش ها: 40 سر موش سفید بزرگ آزمایشگاهی نر، که در 8 گروه به‌صورت تصادفی تقسیم‌بندی شدند: شامل 1) کنترل، 2) دیابتی، 3) دیابتی+ تداومی، 4) دیابتی+ تناوبی، 5) آترواستاتین، 6) تداومی+ آترواستاتین، 7) تناوبی+ آترواستاتین و 8) سالین. موش‌های سفید بزرگ آزمایشگاهی دو نوع تمرین تداومی و تناوبی را 5 روز در هفته و به مدت 8 هفته اجرا کردند. آترواستاتین به‌صورت روزانه با دوز 20 میلی‌گرم به ازای هر کیلوگرم وزن بدن به‌صورت درون صفاقی به آنان تزریق شد. به‌منظور تعیین معنادار‌بودن تفاوت بین گروه‌ها از تحلیل واریانس یک‌طرفه و آزمون تعقیبی توکی استفاده شد.
نتایج: سطوح MMP2 گروه دیابت در سطح معناداری 05/0 نسبت به گروه کنترل افزایش معنا‌دار، و گروه‌های مکمل، تناوبی، تناوبی-مکمل و تداومی-مکمل نسبت به گروه دیابت کاهش معناداری یافت. سطوح VEGF گروه دیابت نسبت به گروه کنترل افزایش معنادار و گروه تناوبی-مکمل نسبت به گروه دیابت کاهش معنادار یافت.
نتیجه‌گیری: این احتمال وجود دارد که تمرین تناوبی در ترکیب با آترواستاتین بتواند از طریق کاهش ژن‌های VEGF و MMP2 بافت آئورت و بهبود آنژیوژنز که در اثر القای دیابت مختل شده بود، دارای اثر حفاظتی بر آئورت باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of two types of exercise training (continuous and intermittent) and atorvastatin on the expression of VEGF and MMP2 genes in aortic tissue of diabetic rats

نویسندگان [English]

  • Maryam Mousapour 1
  • Parvin Farzanegi 1
  • Hajar Abbaszadeh 1
  • Farzane Farokhi 2
1 Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari , Iran
2 Department of Biological Sciences and Technologies, Islamic Azad University, Sari Branch, Sari, Iran
چکیده [English]

Background and Objective: It has been shown that about 80% of deaths in diabetic patients are caused by cardiovascular disorders, which is called diabetic heart disease. The purpose of this research was to investigate the effect of two types of exercise (continuous and periodic) and the drug atorvastatin on the expression of VEGF and MMP2 genes in the aorta tissue of diabetic rats.
Materials and Methods: In this study, 40 male rats, which were randomly divided into 8 groups: 1) control, 2) diabetic, 3) diabetic+continuous, 4) diabetic+intermittent, 5) atorvastatin, 6) continuous + atorvastatin, 7) intermittent + atorvastatin and 8) saline. Rats performed two types of continuous and periodic training 5 days a week for 8 weeks. Atorvastatin was injected intraperitoneally at a daily dose of 20 mg per kilogram of body weight. To determine the significance of the difference between groups, one-way analysis of variance and Tukey's post hoc test were used.
Results: MMP2 levels in the diabetes group significantly increased at the significance level of 0.05 compared to the control group and in the supplement, intermittent, intermittent-supplement and continuous-supplement groups significantly decreased compared to the diabetes group. The levels of VEGF in the diabetes group increased significantly compared to the control and the intermittent-supplement group significantly decreased compared to the diabetes group.
Conclusion: There is a possibility that intermittent exercise in combination with atorvastatin can have a protective effect on the aorta through the reduction of VEGF and MMP2 genes in the aortic tissue and the improvement of angiogenesis that was impaired due to the induction of diabetes.

کلیدواژه‌ها [English]

  • Continuous and intermittent exercises
  • Atorvastatin
  • Angiogenesis
  • VEGF
  • MMP2

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دانشور پزشکی
دوره 31، شماره 6 - شماره پیاپی 169
بهمن و اسفند 1402
صفحه 1-13

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