تجزیه و تحلیل متابولومیکی عضله اسکلتی پس از تمرین تناوبی شدید در موش‌های تغذیه‌شده با رژیم غذایی پرچرب

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

3 مرکز تحقیقات سلولی و مولکولی غدد، پژوهشکده علوم غدد درون ریز، دانشگاه علوم پزشکی شهید بهشتی، تهران

چکیده

مقدمه و هدف: رژیم غذایی پرچرب (HFD)، باعث اختلال در مسیرهای تولید انرژی شده و احتمال بروز مقاومت به انسولین (IR) را افزایش می‌دهد. هدف از تحقیق حاضر بررسی اثر تمرین تناوبی شدید (HIIT) بر نیم‌رخ متابولیکی مرتبط با بروز IR در موش‌های نر تغذیه‌شده با HFD و رژیم غذایی استاندارد (SD) بود.
مواد و روش ها: 32 سر موش صحرایی نر (سن 8-7 هفته) به چهار گروه مساوی شامل HIIT+HFD، HIIT+SD، کنترل دریافت‌کننده HFD (HFD control) و کنترل دریافت‌کننده SD (SD control) تقسیم شدند. سپس مداخله‌هایHIIT  (12 هفته، پنج جلسه در هفته با شدت 90-85 درصد حداکثر اکسیژن مصرفی) و رژیم غذایی اعمال شد و درنهایت، عضله دوقلو به‌منظور بررسی بافتی به‌روش کروماتوگرافی گازی-طیف‌سنجی جرمی برداشته شد. آنالیز داده‌ها با استفاده از آزمون‌های آماری آنالیز واریانس یک‌طرفه، آنالیز کوواریانس یک‌طرفه و کروسکال-والیس در سطح معناداری 05/0> P  انجام شد..
نتایج: میزان اسیدسیتریک عضله دوقلو در گروه  HFD control نسبت به گروه control SD به‌طور معناداری پایین‌تر بود. علاوه‌براین، میزان اسیدسیتریک در گروه‌های HIIT+SD و HIIT+HFD نسبت به گروه HFD control به‌طور معناداری بالاتر بود. بین میزان متابولیت‌های مالات، گلوکز 6-فسفات، ریبوز، گزیلوز و ریبولوز 5-فسفات در بین گروه‌ها تفاوت معناداری مشاهده نشد. همچنین، وزن بدن در گروه‌های HIIT+SD و HIIT+HFD در مقایسه با گروه HFD control به‌طور معناداری پایین‌تر بود.
نتیجه‌گیری: HFD از طریق کاهش میزان اسیدسیتریک عضلانی به‌عنوان واسطه متابولیتی مهم چرخه TCA، در تأمین انرژی عضلات اسکلتی از مسیر هوازی محدودیت ایجاد می‌کند. انجام HIIT می‌تواند به‌واسطه افزایش میزان اسیدسیتریک و کاهش شیب افزایش وزن بدن، در کاهش اختلالات متابولیکی ناشی از HFD مؤثر واقع شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Skeletal muscle metabolomics analysis after high-intensity interval training in rats fed a high-fat diet

نویسندگان [English]

  • Mansoureh Karimi 1
  • Marziyeh Saghebjoo 1
  • Hadi Sarir 2
  • Mehdi Hedayati 3
1 Department of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran
2 Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
3 Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده [English]

Background and Objective: High-fat diet (HFD) causes disturbances in energy production pathways and increases the possibility of insulin resistance (IR). The purpose of this study was to investigate the effect of high-intensity interval training (HIIT) on metabolic profile associated with IR in male rats fed with HFD and standard diet (SD).
Materials and Methods: 32 male rats (aged 7-8 weeks) were divided into four equal groups including HIIT+HFD, HIIT+SD, HFD control, and SD control. Then, HIIT (12 weeks, five sessions per week at intensity of 85-90% of maximum oxygen consumption) and diet interventions were applied and finally, the gastrocnemius muscle was removed for tissue examination by gas chromatography-mass spectrometry. One-way analysis of variance, one-way analysis of covariance, and Kruskal-Wallis tests were used for data analysis at a significance level of P < 0.05.
Results: The level of citric acid in the gastrocnemius muscle was significantly lower in the HFD control group than in the SD control group. In addition, the level of citric acid in HIIT+SD and HIIT+HFD groups was significantly higher than in the HFD control group. There was no significant difference between the levels of malate, glucose 6-phosphate, ribose, xylose, and ribulose 5-phosphate metabolites among the groups. Also, the body weight in the HIIT+SD and HIIT+HFD groups was significantly lower than the HFD control group.
Conclusion: HFD limits the aerobic energy provision of skeletal muscles by reducing the level of muscle citric acid as an important intermediate metabolite in the TCA cycle. HIIT can be effectively reduced metabolic disorders caused by HFD by increasing the level of citric acid and reducing the slope of body weight gain.

کلیدواژه‌ها [English]

  • High-intensity interval training
  • High-fat diet
  • Citric acid
  • Pentose phosphate
  • Metabolomics

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