اثر متوپرولول بر فعالیت حیاتی سلولهای سرطانی خون انسان

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه ایمنی شناسی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: : متوپرولول به‌عنوان یک بتابلوکر، کاربرد فراوانی در درمان برخی بیماری‌های قلبی از جمله فشار خون و انفارکتوس دارد. اثرات سیتوتوکسیک، ضدالتهابی و ضدتوموری متوپرولول گزارش شده‌اند. هدف این مطالعه بررسی اثر متوپرولول بر فعالیت حیاتی رده‌های سلولی سرطانی خون انسان است.
مواد و روش ها: سلول‌های سرطانی خون انسان MOLT-4)  JURKAT, و (U937  در محیطRPMI  کامل کشت داده شدند و سپس به تعداد well /cell  104´3 در گروه‌های پنج چاهکی درون پلیت‌های کشت 96 چاهکی در محیط کشت RPMI حاوی FCS 10% تحت تیمار با غلظت‌های مختلف متوپرولول (μg/ml 1000- 1) در فواصل زمانی 24، 48 و 72 ساعت قرار گرفتند. نهایتاً فعالیت حیاتی سلول‌ها با روش رنگ‌آمیزی تریپان بلو بررسی شد. نتایج حاصله بین گروه تست و کنترل با آزمون آنالیز واریانس مورد مقایسه قرار گرفت.
نتایج: فعالیت حیاتی هر سه رده سلولی پس از 48 ساعت تیمار در مجاورت غلظتμg/ml   1000 از متوپرولول به‌طور معنا‌داری در مقایسه با کنترل کاهش یافت. متوپرولول پس از 72 ساعت تیمار، فعالیت حیاتی دو رده
 MOLT-4 وJURKAT را در غلظت μg/ml 100³ و رده U937 را در غلظت μg/ml 500³ به‌طور معنا‌داری در مقایسه با کنترل کاهش داد.
نتیجه‌گیری: متوپرولول فعالیت حیاتی رده‌های سلولی سرطان خون انسان را به‌صورت وابسته به غلظت و زمان کاهش می‌دهد. اثرات ضدتوموری متوپرولول ممکن است تا حدی ناشی از کاهش فعالیت حیاتی سلول‌ها توسط این دارو باشد؛ بنابراین متوپرولول می‌تواند برای درمان سرطان خون کاربرد بالقوه داشته باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of metoprolol on viability of human blood cancerous cells

نویسندگان [English]

  • Baran Hajatbeigi
  • Fatemeh Hajghasemi
Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran
چکیده [English]

Background and Objective: Metoprolol as a β-Blocker is widely used in treatment of some cardiovascular diseases such as hypertension and myocardial infarction. Moreover, cytotoxic, anti-inflammation, anti-angiogenic and anti-tumor effects of metoprolol have been reported. The intention of this study was to examine metoprolol effect on viability of human blood cancerous cells in vitro.
Materials and Methods: Human leukemic T cells (MOLT-4 and JURKAT) and monocyte (U937) were cultured in Roswell Park Memorial Institute (RPMI) complete medium. Then, the cells (3 ×10 4 cell/well) were treated with different concentrations of metoprolol (1-1000 μg/ml) for 24, 48 and 72 hours. Finally, the viability of cells was determined using trypan blue dye exclusion assay. Analysis of variance (ANOVA) was used to compare the cell viability between different groups.
Results: Metoprolol significantly decreased the viability of all cell lines at 1000 μg/ml concentration after 48 hours incubation time compared with control group. Besides, metoprolol significantly reduced the viability of MOLT-4 and Jurkat cells at ≥ 100 μg/ml concentrations and U937 ≥ 500 μg/ml after 72 hours incubation time in comparison with control group.
Conclusion: Based on results of the present study, metoprolol decreased viability of human blood cancerous cells dose and time dependently. Therefore, anti-tumoral effects of metoprolol reported by other investigators might be partly due to its reducing the viability of cancerous cells. So, metoprolol may have potential implication in therapy of blood tumors along with other cancers.

کلیدواژه‌ها [English]

  • Metoprolol
  • Viability
  • Leukemia

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دانشور پزشکی
دوره 31، شماره 4 - شماره پیاپی 167
مهر و آبان 1402
صفحه 38-46

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